Determination of abamectin and doramectin in sheep faeces using HPLC with ﬂuorescence detection

Kolar, Lucija; Kuz̆ner, Jernej; Eržen, Nevenka Kožuh

doi:10.1002/bmc.306

Cited by 32 publications

(20 citation statements)

References 20 publications

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“…Samples were analyzed in four parallel determinations using a previously reported analytical procedure (Kozˇuh Kolar et al, 2004). The method consisted of the extraction of abamectin or doramectin from the samples followed by clean up of the extracts and then determination by HPLC with fluorescence detection.…”

Section: Reagents and Equipmentmentioning

confidence: 99%

Degradation of Abamectin and Doramectin on Sheep Grazed Pasture

et al. 2005

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Avermectins are widely used veterinary medicines. They bind strongly to faeces in their non-metabolized form and their half-life in faeces depends on field conditions. There are conflicting data regarding the behaviour of avermectins in the environment. Therefore, we investigated the degradation of abamectin and doramectin on sheep grazed pasture under field conditions in soil, soil-faeces and faeces samples from day 6 to day 70 (abamectin) or to day 50 (doramectin) after sheep treatment. Field conditions were recorded periodically during the experiment. Degradation of abamectin in sheep faeces and in soil-faeces was observed until day 60, with small amounts present in faeces until 70 days post treatment. Because the concentration of abamectin residues in soil was very low on day 6 after treatment, further significant degradation could not be measured. The concentration of doramectin in all analysed matrices decreased rapidly until day 50. It can be concluded that high concentrations of both avermectins were present during the first 20 days after treatment and that field conditions have an important role in degradation of avermectins on grazed pasture of treated animals. Clear identification of the consequences of avermectin exposure and the period of the greatest environmental risk will require further investigations.

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Section: Reagents and Equipmentmentioning

confidence: 99%

Degradation of Abamectin and Doramectin on Sheep Grazed Pasture

et al. 2005

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“…Solid-phase extraction SPE has been proved to be a simple and reproducible sample preparation technique and has been successfully applied to the treatment of biological specimens such as blood plasma, urine or feces (Butter et al, 2005;Zang et al, 2005;Kolar et al, 2004). Recently, SPE has been also widely utilized in the filed of minor extraction and L. Yang et al ORIGINAL RESEARCH purification of target components from single or complex herbal mixtures (Lambert et al, 2005;Gokmen et al, 2005), due to its higher selectivity and efficiency of removal of chlorophyll, starch, and other interfering substances.…”

Section: Resultsmentioning

confidence: 99%

Simultaneous quantitative and qualitative analysis of bioactive phenols in Dendrobium aurantiacum var. denneanum by high‐performance liquid chromatography coupled with mass spectrometry and diode array detection

Yang

Wang

Zhang

et al. 2007

Biomedical Chromatography

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A novel high-performance liquid chromatographic method with mass spectrometry and diode array detection method for the simultaneous qualitative and quantitative analysis of bioactive phenols was developed. In total, nine chemically diverse phenols including five bibenzyls, three phenanthrenes and a coumarin were unambiguously identified in Dendrobium aurantiacum var. denneanum by comparison with the available references or reported data according to their retention behaviors, UV spectra and fragmentations of ESI-MS. The contents of the four main phenolic compounds, moscatilin, gigantol, moscatin and coumarin, in D. aurantiacum var. denneanum from the wild and various cultivated populations were determined by HPLC-UV. The sample preparation involved a rapid and simple procedure based on solid-phase extraction using a C(18) reversed-phase cartridge. The quantitative analysis was performed on a Beckman Coulter ODS column (5 microm, 250 x 4.6 mm) using a linear gradient elution system of acetonitrile-0.5% formic acid. The method was validated for linearity, limits of detection (LOD) and quantification (LOQ), precision and accuracy. Good results were obtained with respect to the overall intra- and inter-day variations (RSD less than 3.22%) and the percentage recoveries (ranging from 90.50 to 99.22%). Notable differences in the contents of phenols were observed among different cultivated populations. The samples colleted in April and May (spring), or October and November (autumn) accumulated much higher contents of phenols than those collected in other seasons.

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“…All LC50, LOEC, and NOEC values are based on nominal concentrations in the Lufa 2.2 test soil. Due to the routine procedure performed in our group (Kolar et al 2004(Kolar et al , 2008, we might expect that actual concentrations do not differ by more than 10% from the nominal concentrations.…”

Section: Toxicity Of Abamectin 919mentioning

confidence: 98%

“…Analyses of samples for bioaccumulation were performed using a modification of the procedure developed by Kolar et al (2004) for the analysis of faeces. All samples were analyzed in four parallel determinations.…”

Section: Bioaccumulationmentioning

confidence: 99%

Toxicity of abamectin to the terrestrial isopod Porcellio scaber (Isopoda, Crustacea)

Kolar

Jemec

Gestel³

et al. 2010

Ecotoxicology

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To determine effects of the antiparasitic veterinary drug abamectin on the isopod Porcellio scaber, animals were exposed for 21 days to Lufa 2.2 soil spiked at concentrations of 3-300 mg/kg dry soil. After exposure, abamectin residues in the isopods were analysed using a novel analytical method. Toxicity was evaluated on different levels of biological organisation: biochemical, cellular and the individual organism. Measurements included glutathione S-transferase (GST) activity and stability of cell membranes in the digestive gland, animal mass gain or loss, food consumption, behaviour and mortality. LC50 for the effect of abamectin on survival of P. scaber was 71 mg/kg dry soil. The most obvious sublethal effects were reduced food consumption and decreased body mass (NOEC 3 mg/kg dry soil). Additionally, loss of digging activity and reduced GST activity (NOEC 30 mg/kg dry soil) and cell membrane destabilization (NOEC 10 mg/kg dry soil) were recorded. Abamectin only slightly accumulated in the isopods, with bioaccumulation factors always being \0.1. Based on these results and current information on environmental levels of abamectin, it is not likely that isopods will be affected by abamectin, but further studies with exposure through faeces are recommended.

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Determination of abamectin and doramectin in sheep faeces using HPLC with ﬂuorescence detection

Cited by 32 publications

References 20 publications

Degradation of Abamectin and Doramectin on Sheep Grazed Pasture

Degradation of Abamectin and Doramectin on Sheep Grazed Pasture

Simultaneous quantitative and qualitative analysis of bioactive phenols in Dendrobium aurantiacum var. denneanum by high‐performance liquid chromatography coupled with mass spectrometry and diode array detection

Toxicity of abamectin to the terrestrial isopod Porcellio scaber (Isopoda, Crustacea)

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