Simultaneous quantitative and qualitative analysis of bioactive phenols in Dendrobium aurantiacum var. denneanum by high‐performance liquid chromatography coupled with mass spectrometry and diode array detection

Abstract

A novel high-performance liquid chromatographic method with mass spectrometry and diode array detection method for the simultaneous qualitative and quantitative analysis of bioactive phenols was developed. In total, nine chemically diverse phenols including five bibenzyls, three phenanthrenes and a coumarin were unambiguously identified in Dendrobium aurantiacum var. denneanum by comparison with the available references or reported data according to their retention behaviors, UV spectra and fragmentations of ESI-MS. The contents of the four main phenolic compounds, moscatilin, gigantol, moscatin and coumarin, in D. aurantiacum var. denneanum from the wild and various cultivated populations were determined by HPLC-UV. The sample preparation involved a rapid and simple procedure based on solid-phase extraction using a C(18) reversed-phase cartridge. The quantitative analysis was performed on a Beckman Coulter ODS column (5 microm, 250 x 4.6 mm) using a linear gradient elution system of acetonitrile-0.5% formic acid. The method was validated for linearity, limits of detection (LOD) and quantification (LOQ), precision and accuracy. Good results were obtained with respect to the overall intra- and inter-day variations (RSD less than 3.22%) and the percentage recoveries (ranging from 90.50 to 99.22%). Notable differences in the contents of phenols were observed among different cultivated populations. The samples colleted in April and May (spring), or October and November (autumn) accumulated much higher contents of phenols than those collected in other seasons.

Moscatilin induces apoptosis of pancreatic cancer cells via reactive oxygen species and the JNK/SAPK pathway

“…denneanum . Its structure was determined using 1H-nuclear magnetic resonance (NMR) and 13C-NMR spectral analyses, and its purity was >98%, as determined by high-performance liquid chromatography analysis as described (20). Moscatilin was dissolved in absolute dimethyl sulfoxide (DMSO), and was further diluted with culture medium on the experimental day.…”

Anti-inflammatory phenanthrene derivatives from stems of Dendrobium denneanum

“…Twelve compounds, including bibenzyls, phenanthrenes, benzoquinones, coumarins, cinnamic acid derivatives, b-sitosterol, and daucosterol, were previously isolated from the stems of D. denneanum (Yang et al, 2006b(Yang et al, , 2007bLiu et al, 2009). Due to the successful cultivation of D. denneanum in recent years, this plant has been a substitute for the other endangered Dendrobium species in clinical use.…”

Fast determination of five components of coumarin, alkaloids and bibenzyls in Dendrobium spp. using pressurized liquid extraction and ultra‐performance liquid chromatography

“…Thus, the quality control of Shihu always is to focus on coumarins, alkaloids and bibenzyls [6][7][8][9]. However, these methods suffered from low resolution, low sensitivity [6,7] and long analytical time, as well as more mobile phase consumption [8,9]. Actually, there was no report for simultaneous determination of these three kinds of compounds in Dendrobium spp.…”

“…Coumarins, alkaloids and bibenzyls, which possess many pharmacological activities such as antitumor [2,3], antimutagenesis [4] and antiasthma [5] actions, are usually considered as the important active compounds in Dendrobium. Thus, the quality control of Shihu always is to focus on coumarins, alkaloids and bibenzyls [6][7][8][9]. However, these methods suffered from low resolution, low sensitivity [6,7] and long analytical time, as well as more mobile phase consumption [8,9].…”