Determination of a Mixture of <i>Plantago lanceolata</i> L. and <i>Salvia officinalis</i> L. by High-Performance Liquid Chromatography with Ultraviolet Detection (HPLC-UV)

Gligor, Felicia Gabriela; Frum, Adina; Vicaş, Laura; Totan, Maria; Roman-Filip, Corina; Dobrea, Carmen Maximiliana

doi:10.1080/00032719.2019.1708373

Cited by 13 publications

(8 citation statements)

References 23 publications

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“…Gligor et al [ 32 ] found in 70% ethyl alcohol extract leaves a content of total phenolics of 45.35 mg GAE mL −1 and total flavonoids equal to 26.49 mg QE mL −1 , similar to our results for TotP at Field in July while the corresponding content of TotF was about 1.6 times higher than our results. However, it must be considered that they performed the extraction with a different solvent.…”

Section: Resultssupporting

confidence: 90%

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Antioxidant Contents in a Mediterranean Population of Plantago lanceolata L. Exploited for Quarry Reclamation Interventions

Sanna

Piluzza

Campesi

et al. 2022

Plants

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Plantago lanceolata L. (plantain) is an interesting multipurpose perennial species whose aerial parts are used in herbal medicine due to its precious phytochemicals and are palatable to animals. Moreover, peculiar traits such as drought tolerance, an extended growth season and a deep root system, make plantain a promising pioneer plant for quarry reclamation based on the use of native species. This study evaluated the effects of different environmental conditions and seasons on the accumulation of the bioactive compounds of its aerial organs. An autochthonous plantain population was grown in three locations in Sardinia (Italy). Leaves, peduncles and inflorescences were collected between October 2020 and July 2021. Phenolic contents and antioxidant capacity were determined. The analysis of the individual phenolic compounds was performed using liquid chromatography. In leaves, the content of total phenolics, antioxidant capacity and total flavonoids were significantly influenced by location and season. Total phenolic content ranged from 65 to 240 g gallic acid equivalent kg−1, whereas total flavonoids were from 16 to about 89 g catechin equivalent kg−1. Neochlorogenic, chlorogenic, cryptochlorogenic acids, verbascoside, diosmin and luteolin were identified in the methanolic extracts of leaves, peduncles and inflorescences. Verbascoside was the main antioxidant isolated from plantain extracts. Results evidenced an increasing accumulation pattern of phenolics from vegetative stage to flowering, followed by a decrement towards the seed ripening as well as site-specific differences with amounts of phenolics even 25% higher for same plantain accession.

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Section: Resultssupporting

confidence: 90%

“…Chlorogenic acid was not detected by Gligor et al [ 32 ] in plantain leaves harvested in Romania. Conversely, they found syringic, cinnamic, caffeic, ferulic acids, rutin, catechin and quercetin that were not detected in our study, but they performed the extraction with 70% ethyl alcohol and the phenological stage was not indicated.…”

Section: Resultsmentioning

confidence: 83%

Antioxidant Contents in a Mediterranean Population of Plantago lanceolata L. Exploited for Quarry Reclamation Interventions

Sanna

Piluzza

Campesi

et al. 2022

Plants

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“…The HPLC-UV method for the identification and quantification of phenolic compounds was employed by using several methods already conducted on plants and food supplements [ 37 , 46 , 47 ].…”

Section: Methodsmentioning

confidence: 99%

Geographic Variability of Berry Phytochemicals with Antioxidant and Antimicrobial Properties

et al. 2022

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The aim of this study was to determine the variability of several chemical compounds and the antioxidant and antimicrobial activities of eight types of berries harvested from two different geographical regions in the same year. The analyses were performed on bilberry, black currant, gooseberry, red currant, raspberry, sea buckthorn, strawberry and sour cherry, which were handpicked during the summer of 2019, in the same periods when they are typically harvested for consumer purposes. Total anthocyanins content (TAC), total flavonoids content (TFC), total polyphenolic compounds (TPC), determination of the Ferric-Reducing Antioxidant Power (FRAP), determination of the DPPH free radical scavenging assay (RSA), determination of nine phenolic compounds by HPLC-UV assay and antimicrobial activity were determined for undiluted hydroalcoholic extracts of all the studied berries. The results showed that the berries from Romania were richer in antioxidant compounds than the berries from Russia. The TPC content varied between 4.13–22.2 mg GAE/g d.w., TFC between 3.33–8.87 mg QE/g d.w. and TAC between 0.13–3.94 mg/g d.w. The highest variability was determined for TPC. Regarding the antioxidant activity assessed by FRAP assay, values were between 6.02–57.23 µmols TE/g d.w. and values for the RSA method between 18.44–83.81%. From the eight types of berries analyzed, bilberries and raspberries had the highest antioxidant activity considering both regions and both determination methods. Not only the type, but also the environmental and cultivation conditions in which the berries grow, can lead to variations in their chemical composition. The extracted polyphenolic compounds from the studied berries showed antibacterial properties on pathogens, such as Escherichia coli, Bacillus subtilis and Staphyloccocus aureus. The inhibitory action on Salmonella typhi and fungi Candida albicans and Aspegillus niger was absent to very low. The antimicrobial activity of the hydroalcoholic extracts was dependent on the provenance of the berries, too.

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“…The HPLC-DAD method for the qualitative and quantitative assessment of the ten phenolic compounds analyzed was obtained by using methods already applied on plants and food supplements [ 38 , 40 , 41 ]. The analysis was carried out on an Agilent technologies 1200 series HPLC system equipped with a degasser, a quaternary pump, a diode array detector, a thermostatted column compartment, and an autosampler.…”

Section: Methodsmentioning

confidence: 99%

Valorization of Grape Pomace and Berries as a New and Sustainable Dietary Supplement: Development, Characterization, and Antioxidant Activity Testing

et al. 2022

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Grape pomace and berries represent natural sources of phytochemicals that can increase the quality of life of consumers by contributing to the prevention of chronic diseases; thus, the development of a dietary supplement was necessary. The raw material (r.m.) used for the development of the dietary supplement consisted of dried and powdered bilberries (Vaccinium myrtillus L.), red currants (Ribes rubrum L.), and red fermented pomaces (Vitis vinifera L.) from Feteasca Neagra and Cabernet Sauvignon cultivars. The particle size distribution, powder flow, total phenolic content (TPC), HPLC-DAD phenolic profile assessment, and radical scavenging assay (RSA) were employed for the analysis of the raw material. After encapsulation, the average mass and uniformity of mass, the disintegration, and the uniformity of content for the obtained capsules were performed to obtain a high-quality dietary supplement. All the assays performed complied to the compendial requirements and the TPC was determined at 9.07 ± 0.25 mg gallic acid equivalents/g r.m. and RSA at 48.32 ± 0.74%. The highest quantities of phenolic compounds determined were 333.7 ± 0.50 µg/g r.m. for chlorogenic acid, followed by rutin, ferulic acid, and (+)-catechin with 198.9 ± 1.60 µg/g r.m., 179.8 ± 0.90 µg/g r.m. and 118.7 ± 0.75 µg/g r.m., respectively. The results of this study can be used for the manufacturing and assessing of pilot scale-up capsule batches and thinking of quality assurance, we recommend that the industrial batch extracts should be standardized in polyphenols, and the manufacturing process should be validated.

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Determination of a Mixture of Plantago lanceolata L. and Salvia officinalis L. by High-Performance Liquid Chromatography with Ultraviolet Detection (HPLC-UV)

Cited by 13 publications

References 23 publications

Antioxidant Contents in a Mediterranean Population of Plantago lanceolata L. Exploited for Quarry Reclamation Interventions

Antioxidant Contents in a Mediterranean Population of Plantago lanceolata L. Exploited for Quarry Reclamation Interventions

Geographic Variability of Berry Phytochemicals with Antioxidant and Antimicrobial Properties

Valorization of Grape Pomace and Berries as a New and Sustainable Dietary Supplement: Development, Characterization, and Antioxidant Activity Testing

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