Determination of 5-fluorouracil in human plasma by a simple and sensitive reversed-phase HPLC method

Coe, R.A.J.; Earl, Robert A.; Johnson, Thomas; Lee, J.W.

doi:10.1016/0731-7085(96)01832-8

Cited by 20 publications

(18 citation statements)

References 16 publications

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“…Several analytical methods for the detection and quantification of silymarin flavonolignans in human plasma using HPLC, liquid chromatography mass spectrometry (LC/MS), and liquid chromatography-electrochemical detection have been reported [2][3][4]8]. These techniques, except HPLC, require several sample processing steps or derivatization [11]. The accuracy and precision of plasma analysis of drugs is based upon their extraction efficiency from plasma matrix.…”

Section: Introductionmentioning

confidence: 99%

Effect of &#946-Glucuronidase on Extraction Efficiency of Silymarin from Human Plasma Samples Using Validated HPLC-UV Analysis

Usman¹,

Ahmad²,

Dayo³

et al. 2012

Trop. J. Pharm Res

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Section: Introductionmentioning

confidence: 99%

Effect of &#946-Glucuronidase on Extraction Efficiency of Silymarin from Human Plasma Samples Using Validated HPLC-UV Analysis

Usman¹,

Ahmad²,

Dayo³

et al. 2012

Trop. J. Pharm Res

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“…The results obtained with the optimized LLE can be considered satisfactory since the method was precise and accurate. The results were compared with those in the literature, such as the study of Coe et al (1996) that obtained, for 5-FU, an average recovery of 46% and Turci et al (2002), that reported a recovery of 85% for IF and CF in urine, but using a total volume of 30 mL of solvent extractor (Coe et al, 1996;Turci et al, 2002).…”

Section: Confidence Parametersmentioning

confidence: 99%

“…The response of the analytes was variable, as shown in Figure 2, and a satisfactory percentage of recovery for the four analytes was obtained with ethyl acetate. This solvent was the same used in the method of extraction by Turci et al and Coe et al for the analytes ifosfamide and cyclophosphamide, and 5-fluorouracil, respectively (Coe et al, 1996;Turci et al, 2002). The volume of the solvent of 10 mL (Figure 2) was considered optimum since a balance between the efficiency of the extraction and the environmental implications were considered in the present study.…”

Section: Optimized Sample Treatmentmentioning

confidence: 99%

“…Furthermore, the proteins present in the samples are denatured, eliminating the contamination of the chromatographic column (Queiroz, Collins, Jardim, 2001). Coe et al (1996) reported an LLE procedure for the analysis of fluoruracil in plasma samples, using the pH adjustment to 6.0 by adding acetate buffer, followed by a saturated solution of sodium sulfate and 7.0 mL of ethyl acetate, with around 46% of recovery (Coe et al, 1996). For the ifosfamide and cyclophosphamide isomers, Turci et al (2002) proposed an LLE for urine samples in which 5 mL of sample was adjusted to pH 7, then extracted three times with 10 mL of ethyl acetate (Turci et al, 2002).…”

Section: Optimized Sample Treatmentmentioning

confidence: 99%

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Liquid-liquid extraction combined with high performance liquid chromatography-diode array-ultra-violet for simultaneous determination of antineoplastic drugs in plasma

Sanson

Silva

Martins

et al. 2011

Braz. J. Pharm. Sci.

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A liquid-liquid extraction (LLE) combined with high-performance liquid chromatography-diode array detection method for simultaneous analysis of four chemically and structurally different antineoplastic drugs (cyclophosphamide, doxorubicin, 5-fluorouracil and ifosfamide) was developed. The assay was performed by isocratic elution, with a C18 column (5 µm, 250 x 4.6 mm) and mobile phase constituted by water pH 4.0-acetonitrile-methanol (68:19:13, v/v/v), which allowed satisfactory separation of the compounds of interest. LLE, with ethyl acetate, was used for sample clean-up with recoveries ranging from 60 to 98%. The linear ranges were from 0.5 to 100 µg mL -1 , for doxorubicin and 1 to 100 µg mL -1 , for the other compounds. The relative standard deviations ranged from 5.5 to 17.7%. This method is a fast and simple alternative that can be used, simultaneously, for the determination of the four drugs in plasma, with a range enabling quantification of the drugs in pharmacokinetics, bioequivalence and therapeutic drug-monitoring studies.Uniterms: Antineoplastic drugs/simultaneous determination in plasma. Liquid-liquid extraction. Highperformance liquid chromatography/diode array detector.Um método de extração líquido-líquido (ELL) combinado com cromatografia líquida de alta eficiência-detector de arranjo de diodos foi desenvolvido para análise simultânea de quatro fármacos antineoplásicos quimicamente e estruturalmente diferentes (ciclofosfamida, doxorrubicina, fluoruracila e ifosfamida). O estudo foi realizado sob condições isocráticas, com coluna C18 (5µm, 250 x 4.6 mm) e fase móvel constituída por água pH 4.0-acetonitrila-metanol (68:19:13, v/v/v), que permitiu separação satisfatória dos analitos de interesse. A ELL, com acetato de etila, foi utilizada para limpeza da amostra, com recuperação variando de 60 a 98%. As faixas foram lineares de 0,5 a 100 µg mL -1 para doxorrubicina e 1 a 100 µg mL -1 para os outros compostos. O desvio padrão relativo variou de 5,5 a 17,7%. Este método é uma alternativa rápida e simples que pode ser usado, simultaneamente, para a determinação dos quatro fármacos em plasma, com uma faixa que permite quantificá-los em estudos de farmacocinética, bioequivalência e monitorização terapêutica.Unitermos: Fármacos antineoplásicos/determinação simultânea em plasma. Extração líquido-líquido. Cromatografia líquida de alta eficiência/detector de arranjo de diodos.

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“…A simple and fast LLE double extraction with ethyl acetate demonstrated satisfactory extraction efficiency (Table IV), for 5-FU and TAX, which are analytes (mainly TAX) with lipid solubility, according to Table I. This solvent was previously utilised by Coe et al (1996) for the detection of 5-FU in plasma samples and by Satanic et al (2000) for the detection of TAX in environmental samples. Although the recovery for TAX at level 2 of µg mL -1 was 71.3%, the analyses were precise, and at the high and low levels, the extraction efficiency was > 89.0%.…”

Section: Validationmentioning

confidence: 99%

Simultaneous detection of three antineoplastic drugs on gloves by liquid chromatography with diode array detector

Alcântara

Vitor

Vieira

et al. 2010

Braz. J. Pharm. Sci.

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The aim of this study was to develop a method for simultaneous detection of antineoplastic drugs on gloves since, in occupational exposure, the main contamination route is through dermal contact, which may occur via prolonged contact with contaminated surfaces or materials. The assay was performed by liquid chromatography using the following conditions for the detection of 5-fluorouracil (5-FU), methotrexate (MTX) and paclitaxel (TAX): diode array detection and UV quantification at 195 nm for TAX, at 265 nm for 5-FU and at 302 nm for MTX; ODS column (250 x 4 mm, 5 µm) with a similar guard column; mobile phase consisted of water (pH 4)-methanol-acetonitrile (35:15:50, v/v/v) with a flow rate of 1 mL min -1 . The method presented a linear range from 0.25 to 20 µg mL -1 with r 2 higher than 0.99. Repeatability was ≤15% and satisfactory extraction efficiency was obtained when liquid-liquid extraction with ethyl acetate was used for 5-FU and TAX. Satisfactory solid phase extraction was also achieved with C18 cartridges and elution with methanol for MTX. The diode array detector allowed drug quantification at a concentration ≥ 0.25 µg mL -1 in samples, although detection was possible in samples that presented values of around 0.1 µg mL -1 . The results obtained suggest that the method developed can be applied for the simultaneous determination of the drugs studied and can be considered useful in exposure assessment for health care workers.Uniterms: Antineoplastic drugs/detection. Gloves/toxicologial analysis. High Performance Liquid Chromatography/toxicologial analysis. Occupational exposure.O objetivo deste estudo foi desenvolver um método para a detecção simultânea de antineoplásicos em luvas, uma vez que, em exposições ocupacionais, a principal via de introdução é a dérmica, por meio de contato prolongado com superfícies e/ou materiais contaminados com tais fármacos. A tecnica de detecção utilizada foi a cromatografia líquida de alta eficiência, com detector de aranjo de diodos, nas seguintes condições: para a detecção de 5-fluorouracila (5-FU), metotrexato (MTX) e paclitaxel (TAX): detecção e quantificação de TAX a 195 nm, de 5-FU a 265 nm e de MTX a 302 nm; coluna ODS (250 x 4 mm, 5 mm), com pré-coluna similar; fase móvel constituída de água (pH 4)-metanol-acetonitrila (35:15:50, v/v/v), na vazão de 1 mL min -1. O método apresentou uma faixa linear de 0,25-20 mg mL -1 , com r 2 > 0,99. O desvio-padrão relativo, para a avaliação da repetibilidade foi ≤ 15%; a recuperação foi satisfatória, empregando extração líquido-líquido, com acetato de etila, para a 5-FU e TAX e extração em fase sólida, com cartuchos de C18 e eluição com metanol, para MTX. O detector de arranjo de diodos permitiu a quantificação dos fármacos, quando presentes nas amostras em concentração ≥ 0,25 mg mL -1 , embora a detecção foi possível nas amostras que apresentaram valores em torno de 0,1 mg mL -1 . Os resultados obtidos sugerem que o método desenvolvido pode ser aplicado para a determinação simultânea dos fármacos estudados, constituindo ferr...

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Determination of 5-fluorouracil in human plasma by a simple and sensitive reversed-phase HPLC method

Cited by 20 publications

References 16 publications

Effect of &#946-Glucuronidase on Extraction Efficiency of Silymarin from Human Plasma Samples Using Validated HPLC-UV Analysis

Effect of &#946-Glucuronidase on Extraction Efficiency of Silymarin from Human Plasma Samples Using Validated HPLC-UV Analysis

Liquid-liquid extraction combined with high performance liquid chromatography-diode array-ultra-violet for simultaneous determination of antineoplastic drugs in plasma

Simultaneous detection of three antineoplastic drugs on gloves by liquid chromatography with diode array detector

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