Determination of 26 veterinary antibiotics residues in water matrices by lyophilization in combination with LC–MS/MS

Hu, Fengyang; He, Lili; Yang, Jianwen; Bian, Kui; Wang, Zongnan; Yang, Haicui; Liu, Yahong

doi:10.1016/j.jchromb.2014.01.008

Cited by 67 publications

(27 citation statements)

References 23 publications

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“…In the previously published method for the determination of antimicrobials in water matrices, MeOH-0.1% formic acid aqueous solution (10:90, v/v) was used to dissolve target compounds in extracts of water samples after lyophilization [33]. However, in this study, it did not give satisfactory dissolving recoveries of some antimicrobials from the evaporated residues of vegetable samples, especially SAs.…”

Section: Reconstitutionmentioning

confidence: 79%

Development of a modified QUick, Easy, CHeap, Effective, Rugged and Safe method for the determination of multi-class antimicrobials in vegetables by liquid chromatography tandem mass spectrometry

Hu¹,

Bian²,

Liu³

et al. 2014

Journal of Chromatography A

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Section: Reconstitutionmentioning

confidence: 79%

Development of a modified QUick, Easy, CHeap, Effective, Rugged and Safe method for the determination of multi-class antimicrobials in vegetables by liquid chromatography tandem mass spectrometry

Hu¹,

Bian²,

Liu³

et al. 2014

Journal of Chromatography A

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“…Tiamulin was extracted from serum by acetonitrile with a proportion of 2:1. Concentrations of tiamulin in serum were determined via a high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) method, which was developed by our group and reported previously (22, 23). The recovery and precision were calculated by analysis of spiked samples at three concentration levels (5 replicates of each concentration).…”

Section: Methodsmentioning

confidence: 99%

Pharmacokinetic/Pharmacodynamic Profiles of Tiamulin in an Experimental Intratracheal Infection Model of Mycoplasma gallisepticum

et al. 2016

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Mycoplasma gallisepticum is the most important pathogen in poultry among four pathogenic Mycoplasma species. Tiamulin is a pleuromutilin antibiotic that shows a great activity against M. gallisepticum and has been approved for use in veterinary medicine particularly for poultry. However, the pharmacokinetic/pharmacodynamics (PK/PD) profiles of tiamulin against M. gallisepticum are not well understood. Therefore, in the current studies, we investigated the in vivo PK/PD profiles of tiamulin using a well-established experimental intratracheal infection model of M. gallisepticum. The efficacy of tiamulin against M. gallisepticum was studied in 8-day-old chickens after intramuscular (i.m.) administration at 10 doses between 0–80 mg/kg. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to evaluate the PK parameters of tiamulin following i.m. administration at doses of 5, 40, and 80 mg/kg in Mycoplasma gallisepticum-infected neutropenic chickens. Real-time PCR (RT-PCR) was used for quantitative detection of M. gallisepticum. The MIC of tiamulin against M. gallisepticum strain S6 was 0.03 μg/mL. The PK/PD index, AUC24h/MIC, correlated well with the in vivo antibacterial efficacy. The in vivo data suggest that animal dosage regimens should supply AUC24h/MIC of tiamulin of 382.68 h for 2 log10 ccu equivalents M. gallisepticum reduction. To attain that goal, the administered dose is expected to be 45 mg/kg b.w. for treatment of M. gallisepticum infection with an MIC90 of 0.03 μg/mL.

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“…Previous studies found that antibiotics would hardly be completely absorbed by animals (Hu et al 2014), resulting in the presence of antibiotics as parental forms in animal waste (Zhao et al 2010). The antibiotics and resistant bacteria in animal gut can enter into aquatic and soil environment (Chen et al 2014;Kay et al 2005;Salyers et al 2004;UdikovicKolic et al 2014) through several pathways including Responsible editor: Robert Duran Biao Chen, Lijun Hao, Na Wang and Boping Ye contributed equally to this work.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of antibiotic resistance genes of wastewater and surface water in livestock farms of Jiangsu Province, China

Chen

Lü

Guo

et al. 2015

Environ Sci Pollut Res

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The overuse of antibiotics in livestock farms is general, leading to a wide distribution of antibiotic resistance genes (ARGs) in aquatic environment adjacent to livestock farms. However, researches of the distribution and types of ARGs in aquatic environment of China are still in the initial stage. In this study, wastewater and surface water samples were collected from 12 livestock farms (four pig farms, four cattle farms, and four chicken farms) in Jiangsu Province of China. The prevalence, abundance, and distribution of 22 ARGs were investigated, which were categorized into six groups, including nine tetracyclin resistance genes, three sulfonamides resistance genes, three quinolone resistance genes, two macrolide resistance genes, three aminoglycoside resistance genes, and two multidrug resistance genes, employing quantitative real-time PCR (qPCR). The results suggested that all of the 22 ARGs were detected in samples. Sul1, sul2, and tetM were the most abundant with the average concentration of 3.84×10 1 copies/16S recombinant RNA (rRNA) gene copies, 1.62 × 10 1 copies/16S rRNA gene copies, 2.33 × 10 1 copies/16S rRNA gene copies, respectively. Principle component analysis revealed that the comprehensive pollution of ARGs in northern Jiangsu was more serious. ARGs in wastewater were more abundant when compared to that in surface water. A preliminary study regarding the fate of ARGs after an aerobiotic process showed that tetA, tetC, sul1, sul2, oqxB, and qnrS were significantly increased. And, among the tetracycline resistance genes, the efflux pump genes were enriched while the ribosomal protection protein encoding genes were decreased in the aerobiotic process. The prevalance of ARGs in water environment is of concern; more surveillance is required to determine the pollution level and pattern of antibiotic resistance genes.

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Determination of 26 veterinary antibiotics residues in water matrices by lyophilization in combination with LC–MS/MS

Cited by 67 publications

References 23 publications

Development of a modified QUick, Easy, CHeap, Effective, Rugged and Safe method for the determination of multi-class antimicrobials in vegetables by liquid chromatography tandem mass spectrometry

Development of a modified QUick, Easy, CHeap, Effective, Rugged and Safe method for the determination of multi-class antimicrobials in vegetables by liquid chromatography tandem mass spectrometry

Pharmacokinetic/Pharmacodynamic Profiles of Tiamulin in an Experimental Intratracheal Infection Model of Mycoplasma gallisepticum

Prevalence of antibiotic resistance genes of wastewater and surface water in livestock farms of Jiangsu Province, China

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