Development of a modified QUick, Easy, CHeap, Effective, Rugged and Safe method for the determination of multi-class antimicrobials in vegetables by liquid chromatography tandem mass spectrometry

Hu, Fengyang; Bian, Kui; Liu, Yahong; Su, Yijuan; Zhou, Tong; Song, Xuqin; He, Lili

doi:10.1016/j.chroma.2014.09.074

Cited by 38 publications

(30 citation statements)

References 34 publications

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“…Tiamulin was extracted from serum by acetonitrile with a proportion of 2:1. Concentrations of tiamulin in serum were determined via a high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) method, which was developed by our group and reported previously (22, 23). The recovery and precision were calculated by analysis of spiked samples at three concentration levels (5 replicates of each concentration).…”

Section: Methodsmentioning

confidence: 99%

Pharmacokinetic/Pharmacodynamic Profiles of Tiamulin in an Experimental Intratracheal Infection Model of Mycoplasma gallisepticum

et al. 2016

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Mycoplasma gallisepticum is the most important pathogen in poultry among four pathogenic Mycoplasma species. Tiamulin is a pleuromutilin antibiotic that shows a great activity against M. gallisepticum and has been approved for use in veterinary medicine particularly for poultry. However, the pharmacokinetic/pharmacodynamics (PK/PD) profiles of tiamulin against M. gallisepticum are not well understood. Therefore, in the current studies, we investigated the in vivo PK/PD profiles of tiamulin using a well-established experimental intratracheal infection model of M. gallisepticum. The efficacy of tiamulin against M. gallisepticum was studied in 8-day-old chickens after intramuscular (i.m.) administration at 10 doses between 0–80 mg/kg. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to evaluate the PK parameters of tiamulin following i.m. administration at doses of 5, 40, and 80 mg/kg in Mycoplasma gallisepticum-infected neutropenic chickens. Real-time PCR (RT-PCR) was used for quantitative detection of M. gallisepticum. The MIC of tiamulin against M. gallisepticum strain S6 was 0.03 μg/mL. The PK/PD index, AUC24h/MIC, correlated well with the in vivo antibacterial efficacy. The in vivo data suggest that animal dosage regimens should supply AUC24h/MIC of tiamulin of 382.68 h for 2 log10 ccu equivalents M. gallisepticum reduction. To attain that goal, the administered dose is expected to be 45 mg/kg b.w. for treatment of M. gallisepticum infection with an MIC90 of 0.03 μg/mL.

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Section: Methodsmentioning

confidence: 99%

Pharmacokinetic/Pharmacodynamic Profiles of Tiamulin in an Experimental Intratracheal Infection Model of Mycoplasma gallisepticum

et al. 2016

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“…[1][2][3] Matrix effects (MEs) can be classied into two categories: suppress the response of the analyte and enhance the response. 10 Moreover, several approaches to decrease MEs and improve recoveries have also been described, including: (1) several modied QuEChERS sample preparation methods; [11][12][13][14][15][16][17][18][19][20] (2) the internal standard method; 21 (3) dilution of matrix extraction; 4,8 (4) the microow approach 22 and (5) postcolumn infusion. [4][5][6] Once matrix components coeluted with the analyte, competition may occur between the matrix and analyte during the electrospray process.…”

Section: Introductionmentioning

confidence: 99%

Reduction of matrix effects through a simplified QuEChERS method and using small injection volumes in a LC-MS/MS system for the determination of 28 pesticides in fruits and vegetables

Wang

Zhu³

et al. 2016

Anal. Methods

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A simplified QuEChERS method coupled with a small injection volume was developed for the simultaneous determination of 28 pesticides in 6 matrices (apples, cucumbers, tomatoes, luffa, cabbages, and eggplants) using LC-MS/MS. In the sample procedure, acetonitrile with 0.1% (v/v) formic acid was used to extract and no depurative powder was used. Three fortified levels (10, 50, and 100 mg kg À1 ) were determined and the recoveries of 168 analyte/matrix combinations were in the range of 60-120% except for cyromazin, pendimethalin, and fenpropathrin. Half of the 168 LOQs were below 0.1 mg kg À1 , and 29 LOQs were above 1 mg kg À1 . Moreover, four relationships between signal suppression and injection volume were observed ranging from 0.5 mL to 15 mL. For many analyte/matrix combinations, the matrix effects could be reduced to less than 20% if the injection volume was less than a critical value (named critical volume).The critical volume depending on the initial extent of matrix effects was explored and the conclusion was: for weak or medium MEs, usually #2 mL injection volume was needed and for several weak MEs, injection volume # 5 mL can reduce the matrix effect to a negligible level.

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“…They are also time consuming and usually have a high false positive rate. With recent advances in chromatographic and spectroscopic techniques, HPLC [10,11], LC-MS [12,13], LC-MS/MS [14][15][16][17][18][19][20], and LC coupled to high-resolution MS [21,22] have become prevalent. Generally, antimicrobial residues in animal-derived food are present in trace levels (ng/g).…”

Section: Introductionmentioning

confidence: 99%

Freeze–thaw approach: A practical sample preparation strategy for residue analysis of multi‐class veterinary drugs in chicken muscle

Zhang

et al. 2018

J of Separation Science

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Seven drugs from different classes, namely, fluoroquinolones (enrofloxacin, ciprofloxacin, sarafloxacin), sulfonamides (sulfadimidine, sulfamonomethoxine), and macrolides (tilmicosin, tylosin), were used as test compounds in chickens by oral administration, a simple extraction step after cryogenic freezing might allow the effective extraction of multi-class veterinary drug residues from minced chicken muscles by mix vortexing. On basis of the optimized freeze-thaw approach, a convenient, selective, and reproducible liquid chromatography with tandem mass spectrometry method was developed. At three spiking levels in blank chicken and medicated chicken muscles, average recoveries of the analytes were in the range of 71-106 and 63-119%, respectively. All the relative standard deviations were <20%. The limits of quantification of analytes were 0.2-5.0 ng/g. Regardless of the chicken levels, there were no significant differences (P > 0.05) in the average contents of almost any of the analytes in medicated chickens between this method and specific methods in the literature for the determination of specific analytes. Finally, the developed method was successfully extended to the monitoring of residues of 55 common veterinary drugs in food animal muscles.

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Development of a modified QUick, Easy, CHeap, Effective, Rugged and Safe method for the determination of multi-class antimicrobials in vegetables by liquid chromatography tandem mass spectrometry

Cited by 38 publications

References 34 publications

Pharmacokinetic/Pharmacodynamic Profiles of Tiamulin in an Experimental Intratracheal Infection Model of Mycoplasma gallisepticum

Pharmacokinetic/Pharmacodynamic Profiles of Tiamulin in an Experimental Intratracheal Infection Model of Mycoplasma gallisepticum

Reduction of matrix effects through a simplified QuEChERS method and using small injection volumes in a LC-MS/MS system for the determination of 28 pesticides in fruits and vegetables

Freeze–thaw approach: A practical sample preparation strategy for residue analysis of multi‐class veterinary drugs in chicken muscle

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