Determination of 2′-deoxy-5-iodouridine and its metabolite 5-iodouracil by high-performance liquid chromatography with ultraviolet absorbance detection in human serum

HPLC in biopharmaceutical investigations of drugs representing pyrimidine derivatives (A review)

“…The calibration plot was linear within 6.25 -400 mg/ml for trifluridine and 2.5 -400 mg/ml for 5-trifluoromethyluracil. Belotto et al [109] used reversed-phase HPLC with 0.01 M potassium phosphate buffer -acetonitrile (19 : 1) as the mobile phase to determine idoxuridine and its metabolite (5¢-iodouracil) in human blood serum and achieved a detection level of 80 ng/ml for both compounds (with a linearity range from 100 to 2000 ng/ml). Nguyen and Notary [111] succeeded in separating 11 compounds (including methyluracil, methyluridines, uracil arabinoside, cytosine arabinoside, uracils, cytidines, and cytosines) using an Ultrasphere C-18 column (205´4.6 mm, 5 mm) eluted by 0.5 mM sodium dihydrogen phosphate and 0.5 mM sodium hydrogen phosphate with methanol (92 : 8 for methyl derivatives and 95 : 5 for the other pyrimidines).…”