Determination of 2,3-Butylene Glycol in Fermentations

Abstract

production of 2,3-butylene glycol is a property common to many microorganisms in the dissimilation

Diversion of the Normal Heterolactic Dissimilation by Addition of Hydrogen Acceptors

“…Sugar was determined at the time of inoculation and at the conclusion of the fermentation by the Munson and Walker (1906) method after deproteinating with phosphotungstic acid and removing the acetaldehyde by distillation. Acetylmethylcarbinol and 2,3-butyleneglycol were determined by the methods of Van Niel (1927) and Brockmann and Werkman (1933), respectively, as modified by Stahly and Werkman (1936). Corrections for the effect of acetylmethylcarbinol on the determinations of glucose, ethyl alcohol and 2,3-butyleneglycol were made.…”

A New Type of Glucose Fermentation by Clostridium thermoaceticum

“…Non-volatile acids, calculated as lactic acid, were determined by extraction of the residue from the volatile acid distillation with ether in a Kutscher-Steudel apparatus and titration of the extract with barium hydroxide. Glucose was determined by the method of Somogyi (1937); formic acid by the method of the American Association of Official Agricultural Chemists (1935); ethyl and butyl alcohols by the method of Johnson (1932); carbon by a modification of the method of Friedemann and Kendall (1929); acetylmethylcarbinol by the method of Langlykke and Peterson (1937); 2,3-butylene glycol by a modification of the method of Brockman and Werkman (1933); isopropyl alcohol by the method of Allgeier and Tatum as quoted by Langlykke, Peterson and McCoy (1935); and acetone by a modification of Goodwin's (1920) method. Carbon dioxide was absorbed in potassium hydroxide.…”

Reductive Processes of Clostridium butylicum and the Mechanism of Formation of Isopropyl Alcohol

“…For the determination of 2,3-butylene glycol an aliquot of the culture was neutralized with sodium hydroxide, taken up with plaster of Paris, and extracted with ether for 48 hours. Water was added to the extract, the ether was distilled off and the aqueous solution was oxidized to acetaldehyde by acid periodate (Brockmann and Werkman (1933)). The acetaldehyde was absorbed and determined as in the lactic acid method of Friedemann and Graeser (1933).…”