Determination of 14 aminoglycosides by LC-MS/MS using molecularly imprinted polymer solid phase extraction for clean-up

Abstract

An LC-MS/MS method for screening 14 aminoglycosides in foodstuffs of animal origin is presented. Its scope includes raw materials and processed ingredients but also finished products composed of milk, meat, fish, egg or fat. Aminoglycosides are extracted in an acidic aqueous solution, which is first recovered after centrifugation, then diluted with a basic buffer and finally purified by molecularly imprinted polymer-solid phase extraction (MIP-SPE). Analytes are detected within 8 min by ion-pair reversed phase LC-MS/MS. Due to the large range of foodstuffs involved, the variability of matrix effects led to significant MS signal variations. This was circumvented by systematically extracting each sample twice, i.e. 'unspiked' and 'spiked' at the screening target concentration of 50 µg kg. The method was validated according to the European Community Reference Laboratories Residues Guidelines giving false-negative and false-positive rates ≤3% for all compounds. Ruggedness of the method was further demonstrated in quality control operations by a second laboratory. The 14 aminoglycosides in water-based standard solutions were stable for up to 6 months when stored at either -80°C, -20°C or at 4°C storage temperatures.

MIPs for commercial application in low-cost sensors and assays – An overview of the current status quo

“…Early commercialization of MIPs was aimed at application in purification and separation, pioneered by companies such as MIP Technologies, POLYINTELL (AFFINIMIPSPE products), and Supelco (previously Sigma Aldrich, now Merck). Acros (SupelMIP) for instance, offers MIPs cartridges for purchase for the solid phase extraction (SPE) of a number of environmental contaminants [ 244 ]. Aspira Biosystems sells MIPs, made by epitope imprinting that are able to selectively capture microorganisms [ 245 ].…”

Recent Advances in Electrochemical Sensors Based on Molecularly Imprinted Polymers and Nanomaterials

“…MIPs are excellent materials with high selectivity and are widely used for sample preparation in bio analytical methods, as well as in sensors , chromatography , solid phase extraction , catalysis , and drug delivery and others.…”

Simultaneous determination of ten aminoglycoside antibiotics in aquatic feeds by high‐performance liquid chromatography quadrupole‐orbitrap mass spectrometry with pass‐through cleanup

“…To avoid the complicated procedures, mass spectrometry (MS) is an alternative for AGs analysis. Liquid chromatography‐MS (LC‐MS) and liquid chromatography tandem MS (LC‐MS/MS) are widely used methods for single or multi‐AGs residues detection with high selectivity and sensitivity . Recently, high‐resolution MS (HRMS) is another promising approach for multiresidues detection, which is becoming popular due to its superior selectivity in complex sample matrix .…”

“…Liquid chromatography-MS (LC-MS) and liquid chromatography tandemMS(LC-MS/MS)arewidelyusedmethodsforsingleor multi-AGsresiduesdetectionwithhighselectivityandsensitivity. [12][13][14] Recently, high-resolution MS (HRMS) is another promising approach for multiresidues detection, which is becoming popular due to its superior selectivity in complex sample matrix. 15,16 HPLC quadrupole-orbitrap MS (HPLC-Q/Orbitrap MS) is a high-resolution mass spectrometer containingorbitrapmassanalyzer.Thisinstrumentperformsbetter sensitivity and specificity as its high-accurate mass measurement in qualitative and quantitative analysis.…”