Determination of 1-deoxynojirimycin in Morus alba L. leaves by derivatization with 9-fluorenylmethyl chloroformate followed by reversed-phase high-performance liquid chromatography

Kim, Jin-Won; Kim, Soo-Un; Lee, Heui Sam; Kim, Iksoo; Ahn, Mi Young; Ryu, Kang Sun

doi:10.1016/s0021-9673(03)00728-3

Cited by 150 publications

(96 citation statements)

References 24 publications

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“…The wavelengths to detect chlorogenic acid and flavonols were 280 nm and 350 nm, respectively. We quantified DNJ according to the method of Kim et al (2003). Standard flavonols, kaempferol 3-(6-rhamnosylglucoside), quercetin 3-(6-malonylglucoside), and kaempferol 3-(6-malonylglucoside) were purified from mulberry leaves as described by Katsube et al (2006).…”

Section: Quantification Of Chlorogenic Acid Flavonols and Dnjmentioning

confidence: 99%

Seasonal Changes in Functional Component Contents in Mulberry (<i>Morus alba</i> L.) Leaves

Sugiyama

Katšube²,

Kôyama³

et al. 2017

Hortic J

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The aim of this study was to determine the best time to harvest mulberry (Morus alba L.) leaves for optimal yields of functional components. Mulberry leaves were analyzed to determine the seasonal changes in the concentrations of functional components, including flavonols, chlorogenic acid, and 1-deoxynojirimycine (DNJ). During the experimental period (May 26-October 16), the ranges of flavonols, chlorogenic acid, and DNJ contents were 1134-2230 mg/100 g dry weight (DW), 616-1014 mg/100 g DW, and 53-199 mg/100 g DW, respectively. There were high flavonols contents in mulberry leaves from late May to early July, from early to mid-August, and after late September. There were high chlorogenic acid contents from late May to early July, and after late September. From late September, the flavonols contents increased over time, and showed the highest level on October 16, the last day of the experimental period. The increased flavonols contents after late September were due to the synergistic effects of solar radiation and lower temperatures. The DNJ content showed a bell-shaped curve, peaking in mid-August, and the DNJ content was strongly correlated with total temperature. The flavonols and chlorogenic acid contents in mulberry leaves were higher after late September when the temperatures decreased and there was still high solar radiation, while the DNJ content peaked in August when temperatures were highest. The results indicated that August is the best time to harvest mulberry leaves to obtain optimal yields of beneficial functional components. If mulberry leaves are harvested twice per year, then the first harvest should be in July to August when temperatures and solar radiation hours are high, and the second harvest should be completed by the end of September.

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Section: Quantification Of Chlorogenic Acid Flavonols and Dnjmentioning

confidence: 99%

Seasonal Changes in Functional Component Contents in Mulberry (<i>Morus alba</i> L.) Leaves

Sugiyama

Katšube²,

Kôyama³

et al. 2017

Hortic J

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“…Preparation and extraction of mulberry tea The preparation of mulberry tea was based on the common production process of green tea, and a methods using an oven was gave (keelrein, Shanghai, China) to improve, the method has the advantages of filter. Between each step, it is necessary to vortexed for 15 s to ensure the solution was evenly blended (Kim et al, 2003). …”

Section: Methodsmentioning

confidence: 99%

Optimization of Microwave-Assisted Technology for Extracting 1-deoxynojirimycin from Mulberry Tea by Response Surface Methodology

Liu

Wang

Liu

et al. 2014

FSTR

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In order to improve the utilization ratio of mulberry tea 1-deoxynojirimycin (DNJ), mulberry tea was prepared based on the common production process of green tea, and corresponding experimental design and statistical analysis were set using Design-Expert software. The results suggested microwave power 602.28 W and processing time 11.41 min were found to be optimal for microwave-assisted extraction DNJ. The confirmatory experiment showed the coincidence rate of detection was 99.58%. It provided a reference for efficient use of mulberry tea DNJ for people who would drink mulberry tea to control blood glucose levels or to prevent diet-induced obesity.

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“…ELSD is the universal detector that responds to nonvolatile compounds and directly detects analytes lacking chromophores. Kim et al (2003) reported the procedures for deriving DNJ using 9-fluorenylmethoxycarbonyl chloride, which targets secondary amino groups in DNJ, followed by reverse-phase HPLC using a fluorescence detector. Furthermore, several other methods, such as HILIC-MS/MS (Nakagawa et al, 2010) and anion-exchange chromatography with pulsed amperometric detection methods (Yoshihashi et al, 2010), have been developed for measuring DNJ levels.…”

Section: Methods For Determining Dnj In Mulberrymentioning

confidence: 99%

Development of Mulberry Leaf Extract for Suppressing Postprandial Blood Glucose Elevation

Kimura¹

2011

Hypoglycemia - Causes and Occurrences

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Determination of 1-deoxynojirimycin in Morus alba L. leaves by derivatization with 9-fluorenylmethyl chloroformate followed by reversed-phase high-performance liquid chromatography

Cited by 150 publications

References 24 publications

Seasonal Changes in Functional Component Contents in Mulberry (<i>Morus alba</i> L.) Leaves

Seasonal Changes in Functional Component Contents in Mulberry (<i>Morus alba</i> L.) Leaves

Optimization of Microwave-Assisted Technology for Extracting 1-deoxynojirimycin from Mulberry Tea by Response Surface Methodology

Development of Mulberry Leaf Extract for Suppressing Postprandial Blood Glucose Elevation

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