Determination and quantification of asiaticoside in endophytic fungus from Centella asiatica (L.) Urban

Abstract

Centella asiatica (L.) Urban is a highly considered medicinal plant owing to its secondary metabolites asiaticoside, madecassoside, asiatic acid, and madecassic acid. The asiaticoside, one of the most important constituents of the plant, is a triterpenoid saponin having memory enhancement property. Given its medicinal properties, we isolated and characterized endophytic fungi from this plant with the aim to screen these microorganisms for asiaticoside production. In total, we isolated 13 endophytic fungi from the leaves of the plant, out of which one of the isolates produced asiaticoside. This asiaticoside producing isolate was identified as Colletotrichum gloeosporioides by internal transcribed spacer-based rDNA sequencing. The presence of asiaticoside in ethyl acetate extract of C. gloeosporioides was confirmed by LC-MS. The production of asiaticoside measured in relation to incubation time and subculture generation revealed presence of 62.29 ± 3.36 µg/100 mL of asiaticoside by C. gloeosporioides on the 15th day in first subculture generation followed by a decrease in subsequent generations. A similar trend was also shown by yield and growth curve of C. gloeosporioides. The asiaticoside production and yield were found to be positively correlated. This paper reported the production of asiaticoside by an endophytic fungus C. gloeosporioides for the first time. The present findings definitely provide an impetus to the production of asiaticoside by utilizing the endophytic source. Chemical compound studied in this article: Asiaticoside (PubChemCID: 108062).

Association between dipsacus saponin VI level and diversity of endophytic fungi in roots of Dipsacus asperoides

“…Our results highlight the diversity of endophytic fungi in medicinal plants and their ability to synthesize bioactive secondary metabolites (Gupta et al 2018). They may also guide new approaches to synthesize dipsacus saponin VI from D. asperoides , permitting sustainable development of this important traditional Chinese medicine resource.…”

In vivo Therapeutic Effects and Mechanisms of Hydroxyasiaticoside Combined With Praziquantel in the Treatment of Schistosomiasis Induced Hepatic Fibrosis

“…Schistosomiasis is a fatal disease that threatens human health, and more than 150 million people are infected with schistosomiasis globally (Bylka et al, 2014;Zhou et al, 2016;Fitri et al, 2018a). Previous studies showed that liver fibrosis symptoms due to S. japonicum can persist for a long time after complete disinfestation (Fitri et al, 2018b;Song et al, 2018;He et al, 2019). Patients with advanced schistosomiasis-induced liver fibrosis gradually develop cirrhosis due to egg deposition, and often die of gastrointestinal bleeding and liver failure caused by portal hypertension (Barnett, 2018;Chuah et al, 2019;Lewis and Tucker, 2019).…”

Insight Into Microbial Applications for the Biodegradation of Pyrethroid Insecticides

“…Inaccurate results of these tests usually resulted in wrong isolation and characterization. Development of molecular biology tools have facilitated the isolation and identification of pyrethroid degrading microbes (16S rRNA for bacteria and ITS sequencing for fungi) (Gangola et al, 2018; Gupta et al, 2018). Degradation of pyrethroids is commonly analyzed by chromatographic techniques such as high performance liquid chromatography (HPLC), gas chromatography (GC), and mass spectroscopy (MS) (Castellarnau et al, 2016).…”