Determination and pharmacokinetics of gastrodin in human plasma by HPLC coupled with photodiode array detector

Ju, Xue Hai; Shi, Ying; Liu, Na; Guo, Dong; Chen, Xi

doi:10.1016/j.jchromb.2010.05.034

Cited by 15 publications

(17 citation statements)

References 19 publications

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“…UHPLC/Q-TOF MS exhibited excellent performances for metabolic study with excellent MS accuracy and automated MS E technique. However, previous studies on the in vivo metabolism of gastrodin were mainly focused on gastrodin and p-hydroxybenzyl alcohol ( Ju et al, 2010;Lin et al, 2007;Wang et al, 2002). After analysis, a total of 14 metabolites were detected and identified in rat plasma and urine after intragastric administration of parishin compared with parishin-free samples.…”

Section: Discussionmentioning

confidence: 99%

Analysis of the metabolic profile of parishin by ultra‐performance liquid chromatography/quadrupole‐time of flight mass spectrometry

Tang

Wang

et al. 2015

Biomedical Chromatography

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Parishin is a dominant active ingredient originating from Gastrodia elata Blume, and has good neuroprotective effects against brain disorders. In the present study, the metabolic profile of parishin by in vitro and in vivo experiments was investigated using ultra-high performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry (UHPLC/Q-TOF MS) combined with an automated MS(E) technique. By comparison with reference compounds, accurate mass measurement, the characteristic fragmentation patterns of the parent drug parishin and gastrodin and relevant bio-transformation knowledge, 14 metabolites (seven hydrolyzates and seven derivatives of gastrodin) were detected and identified in rat plasma and urine after intragastric administration of parishin, including processes of hydrolyzation, oxidation, sulfation and glucuronidation. According to the proposed metabolic pathways of parishin, in vitro hydrolytic experiments and metabolic study of gastrodin in rat plasma, it can be inferred that parishin mainly functions as a prodrug and undergoes hydrolysis before being absorbed into the blood. The hydrolyzate, mainly gastrodin, was involved in further metabolism, which was responsible for pharmacological activities of parishin. In conclusion, this work provides valuable information on parishin metabolism using a rapid and reliable UHPLC/Q-TOF MS method, which could be widely used for the metabolic investigation of natural product.

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Section: Discussionmentioning

confidence: 99%

Analysis of the metabolic profile of parishin by ultra‐performance liquid chromatography/quadrupole‐time of flight mass spectrometry

Tang

Wang

et al. 2015

Biomedical Chromatography

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“…Most researchers have mainly focused on the pharmacokinetic study of GAS in rat plasma (Ju, Shi, Liu, Guo, & Cui, ; Yang, Han, Du, & Chen, ; Zhang, Sheng, & Zhang, ) or brain (Lin, Chen, Lee, Wu, & Tsai, ; Wang, Chen, & Zeng, ; Zhang et al, ) after administration of GAS monomer or free GAS in GR extract. However, along with the extensive phytochemical studies, a number of new active components have been identified, especially for GAS conjugates, such as PA, parishin B and parishin C (Chen, Liu, Xiao, Zhang, & Liu, 2016; Li & Chen, ; Wang, Xiao, Liang, & Wei, ).…”

Section: Introductionmentioning

confidence: 99%

Relative tissue distribution and excretion studies of gastrodin and parishin from powder and extract of Gastrodiae Rhizoma in rat by UHPLC‐ESI‐MS/MS

Jiang

Zheng

Gong

et al. 2017

Biomedical Chromatography

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New research has indicated that Gastrodiae Rhizome (GR) has potential anti-diabetic and anti-asthmatic effects in mouse models. On the basis of our previous study of the relative bioavailability of gastrodin (GAS) and parishin (PA) from extract and powder of GR, we performed further research on the tissue distribution and excretion of the two analytes. A reliable bioanalytical method for the quantification of GAS and PA in rat tissues and excretion is required. Chromatographic separation was carried out on a gradient mobile phase of acetonitrile-water with 0.1% formic acid. Calibration curves (1/x weighted) offered satisfactory linearity (r > 0.9835) within 100-3000 ng mL for GAS and (r > 0.9862) within 10-1000 ng mL for PA. The relative standard deviations of the intra-day and inter-day precision were all <14.98%, whilst the relative errors of the intra-day and inter-day accuracy were all within ±14.71%. The matrix effect and recovery values were satisfactory in all of the biological matrices examination. The data of relative differences in tissue distribution and excretion of GAS and PA from powder and extract of GR indicated that higher bioavailabilities for GAS and PA were obtained when a dosage of 4 g kg GR powder was used.

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“…However, the previous study about pharmacokinetics of G. elata mainly focused on the study of GAS [7,20]. GAS was rapidly absorbed and reached the peak concentration about 1 h after oral administration [21]. Then, GAS underwent abundant phase II reactions by conjugation with endogenous molecule (glucuronic acid, sulfuric acid, etc.)…”

Section: Introductionmentioning

confidence: 99%

Pharmacokinetic study of Gastrodia elata in rats

et al. 2015

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The pharmacokinetics of parishin, gastrodin, Gastrodia elata extract and Rhizoma Gastrodiae capsule was investigated by intragastric and/or intravenous administration to rats. Parishin was metabolized into nine metabolites after intravenous administration, and the area under the curve (AUC0-∞) of parishin and its metabolites (except parishin G and parishin E) increased nonlinearly from 72.5 to 220 mg/kg. When combining regression equation with the AUC0-∞ and dose of gastrodin injection, the percent conversion of parishin to gastrodin was obtained as 50 %. Based on multi-active metabolites of parishin in vivo, integrated pharmacokinetic mode was established. It is notable that each metabolite from parishin shares the similar metabolic process at three dosages of parishin and the bioavailability of parishin was approximately 14 %. The integrated pharmacokinetic mode was successfully applied to evaluate the holistic pharmacokinetics of gastrodin injection, G. elata extract and Rhizoma Gastrodiae capsule. The results showed that the holistic pharmacokinetics of gastrodin injection and G. elata extract was closed to that of gastrodin, but for parishin and Rhizoma Gastrodiae capsule, integrated pharmacokinetic parameters were more suitable to evaluate its holistic pharmacokinetics. Graphical abstract Pharmacokinetic study of Gastrodia elata in rats.

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Determination and pharmacokinetics of gastrodin in human plasma by HPLC coupled with photodiode array detector

Cited by 15 publications

References 19 publications

Analysis of the metabolic profile of parishin by ultra‐performance liquid chromatography/quadrupole‐time of flight mass spectrometry

Analysis of the metabolic profile of parishin by ultra‐performance liquid chromatography/quadrupole‐time of flight mass spectrometry

Relative tissue distribution and excretion studies of gastrodin and parishin from powder and extract of Gastrodiae Rhizoma in rat by UHPLC‐ESI‐MS/MS

Pharmacokinetic study of Gastrodia elata in rats

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