Determination and assay validation of pinosylvin in rat serum: application to drug metabolism and pharmacokinetics

Roupe, Kathryn A.; Halls, Steven C.; Davies, Neal M.

doi:10.1016/j.jpba.2004.12.015

Cited by 22 publications

(17 citation statements)

References 12 publications

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“…The disposition pattern of this 3′-hydroxylated stilbene is unique but the disposition shares some similarities with other stilbenes we have previously characterized in our laboratory in the rat model (Remsberg et al, 2007Roupe et al, 2004Roupe et al, , 2005aRoupe et al, ,b, 2006aWang et al, 2008).…”

Section: Disposition and Quantification Of 3'-hydroxypterostilbene Insupporting

confidence: 62%

A high‐performance liquid chromatographic analysis and preliminary pharmacokinetic characterization of 3′‐hydroxypterostilbene in rats

Takemoto

Davies

2009

Biomedical Chromatography

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A high-performance liquid chromatographic method was developed for the analysis of 3'-hydroxypterostilbene. This method involves the use of a Luna C(18) column with ultraviolet detection at 325 nm. The mobile phase consisted of acetonitrile, water and formic acid (50:50:0.01, v/v/v) with a flow rate of 0.8 mL/min. The calibration curves were linear over the range 0.5-100.0 microg/mL. The mean extraction efficiency was between 97.40 and 111.16%. The precision of the assay was 0.196-14.39% (RSD%), and within 15% at the limit of quantitation (0.5 microg/mL). The bias of the assay was <16% and within 15% at the limit of quantitation. This assay was successfully applied to pre-clinical pharmacokinetic samples from rat urine and serum.

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Section: Disposition and Quantification Of 3'-hydroxypterostilbene Insupporting

confidence: 62%

A high‐performance liquid chromatographic analysis and preliminary pharmacokinetic characterization of 3′‐hydroxypterostilbene in rats

Takemoto

Davies

2009

Biomedical Chromatography

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“…Following oral administration of oxyresveratrol dihydrate the pharmacokinetic parameters were as follows: mean half life (t 1/2 ) in urine was ~5.30 h, mean rate of elimination (K E ) was ~0.59 1/h, and the mean fraction excreted in urine unchanged (Fe) was ~0.027%. The disposition pattern of this stilbene is unique but the disposition shares some similarities with other stilbenes we have previously characterized in our laboratory in the rat model (Remsberg et al, 2007Roupe et al, 2004Roupe et al, , 2005aRoupe et al, , b, 2006aWang et al, 2008).…”

Section: Disposition and Quantifi Cation Of Oxyresveratrol In Rat Urinesupporting

confidence: 60%

High‐performance liquid chromatographic analysis: applications to nutraceutical content and urinary disposition of oxyresveratrol in rats

Bertram¹,

Takemoto²,

Remsberg³

et al. 2009

Biomedical Chromatography

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A high-performance liquid chromatographic (HPLC) method was developed for the analysis of the stilbene, oxyresveratrol. This method involves the use of a Luna C(18) column with ultraviolet detection at 320 nm. The mobile phase consisted of acetonitrile, water and formic acid (30 : 70 : 0.04 v/v) with a flow rate of 0.6 mL/min. The calibration curves were linear over the range of 0.5-100.0 microg/mL. The mean extraction efficiency was between 98.9 and 109%. The precision of the assay was 0.069-18.4% (RSD%), and within 20% at the limit of quantitation (0.5 microg/mL). The bias of the assay was <15% and within 15% at the limit of quantitation. This assay was successfully applied to pre-clinical pharmacokinetic samples from rat urine and to nutraceutical product analysis.

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“…Interestingly, we found a number of novel as well as previously documented metabolites derived from PKE in the serum and urine in significant concentrations. These included pinostilbene (a novel identified metabolite of MePS), RV (a known metabolite of PS) [39], and enterolactone, 7-hydroxyenterolactone, and enterodiol (known metabolites of HMR and MR, respectively) [40]. PS, MePS, NTG and MR were present in the serum in high μM concentrations, i.e.…”

Section: Discussionmentioning

confidence: 99%

Novel Lignan and Stilbenoid Mixture Shows Anticarcinogenic Efficacy in Preclinical PC-3M-luc2 Prostate Cancer Model

et al. 2014

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Prostate cancer is the most common cancer of men in the Western world, and novel approaches for prostate cancer risk reduction are needed. Plant-derived phenolic compounds attenuate prostate cancer growth in preclinical models by several mechanisms, which is in line with epidemiological findings suggesting that consumption of plant-based diets is associated with low risk of prostate cancer. The objective of this study was to assess the effects of a novel lignan-stilbenoid mixture in PC-3M-luc2 human prostate cancer cells in vitro and in orthotopic xenografts. Lignan and stilbenoid –rich extract was obtained from Scots pine (Pinus sylvestris) knots. Pine knot extract as well as stilbenoids (methyl pinosylvin and pinosylvin), and lignans (matairesinol and nortrachelogenin) present in pine knot extract showed antiproliferative and proapoptotic efficacy at ≥40 μM concentration in vitro. Furthermore, pine knot extract derived stilbenoids enhanced tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) induced apoptosis already at ≥10 μM concentrations. In orthotopic PC-3M-luc2 xenograft bearing immunocompromized mice, three-week peroral exposure to pine knot extract (52 mg of lignans and stilbenoids per kg of body weight) was well tolerated and showed anti-tumorigenic efficacy, demonstrated by multivariate analysis combining essential markers of tumor growth (i.e. tumor volume, vascularization, and cell proliferation). Methyl pinosylvin, pinosylvin, matairesinol, nortrachelogenin, as well as resveratrol, a metabolite of pinosylvin, were detected in serum at total concentration of 7−73 μM, confirming the bioavailability of pine knot extract derived lignans and stilbenoids. In summary, our data indicates that pine knot extract is a novel and cost-effective source of resveratrol, methyl pinosylvin and other bioactive lignans and stilbenoids. Pine knot extract shows anticarcinogenic efficacy in preclinical prostate cancer model, and our in vitro data suggests that compounds derived from the extract may have potential as novel chemosensitizers to TRAIL. These findings promote further research on health-related applications of wood biochemicals.

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Determination and assay validation of pinosylvin in rat serum: application to drug metabolism and pharmacokinetics

Cited by 22 publications

References 12 publications

A high‐performance liquid chromatographic analysis and preliminary pharmacokinetic characterization of 3′‐hydroxypterostilbene in rats

A high‐performance liquid chromatographic analysis and preliminary pharmacokinetic characterization of 3′‐hydroxypterostilbene in rats

High‐performance liquid chromatographic analysis: applications to nutraceutical content and urinary disposition of oxyresveratrol in rats

Novel Lignan and Stilbenoid Mixture Shows Anticarcinogenic Efficacy in Preclinical PC-3M-luc2 Prostate Cancer Model

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