A high‐performance liquid chromatographic analysis and preliminary pharmacokinetic characterization of 3′‐hydroxypterostilbene in rats

Abstract

A high-performance liquid chromatographic method was developed for the analysis of 3'-hydroxypterostilbene. This method involves the use of a Luna C(18) column with ultraviolet detection at 325 nm. The mobile phase consisted of acetonitrile, water and formic acid (50:50:0.01, v/v/v) with a flow rate of 0.8 mL/min. The calibration curves were linear over the range 0.5-100.0 microg/mL. The mean extraction efficiency was between 97.40 and 111.16%. The precision of the assay was 0.196-14.39% (RSD%), and within 15% at the limit of quantitation (0.5 microg/mL). The bias of the assay was <16% and within 15% at the limit of quantitation. This assay was successfully applied to pre-clinical pharmacokinetic samples from rat urine and serum.

Pharmacologic Activities¬ of 3’-Hydroxypterostilbene: Cytotoxic, Anti-Oxidant, Anti-Adipogenic, Anti-Inflammatory, Histone Deacetylase and Sirtuin 1 Inhibitory Activity

“…The partition coefficient (XLogP) was experimentally investigated using the shake flask method at ambient temperature (23 ± 2°C) with spectrophotometric analysis (4)(5) and by a HPLC analytical assay (6).…”

“…Internal standard (4-methylumbelliferone, 0.10 mL) was added to the dried samples and dried to completion. Samples were resuspended in mobile phase (0.40 mL), injected (0.05 mL) into the system, and the developed analytical method applied (6). The peak area ratios were determined for the octanol and aqueous phase taking into consideration the total volume of solvents for all standards and samples (8); concentrations of unknown samples were determined using linear regression from the standard curve and the XLogP was calculated.…”