Determinants of Genital Human Papillomavirus Detection in a US Population

Peyton, Cheri L.; Gravitt, Patti E.; Hunt, William C.; Hundley, Rosalina; Zhao, Meifen; Apple, Raymond; Wheeler, Cosette M.

doi:10.1086/320696

Cited by 223 publications

(167 citation statements)

References 42 publications

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“…LA utilizes the PGMY09/11 L1 consensus primer system and includes co-amplification of a human cellular target, β-globin (Gravitt et al 2000), as an internal control. Detection and HPV genotyping are achieved using a reverse line-blot method (Gravitt et al 1998;Peyton et al 2001), which includes probes to identify 37 anogenital carcinogenic and non-carcinogenic HPV genotypes (6,11,16,18,26,31,33,35,39,40,42,45,(51)(52)(53)(54)(55)(56)58,59,61,62,64,(66)(67)(68)(69)(70)(71)(72)(73)81,82 subtype [IS39], 82 subtype [W13b], 83, 84, and 89). The only deviation from the LA product insert protocol was to implement an automated sample preparation for extraction of up to 96 specimens at a time on the QIAGEN MDx platform (using the MinElute Media MDx Kit and manufacturer's instructions) rather than processing 24 specimens per batch with the manual vacuum method (Castle et al 2006).…”

Section: Hpv Testingmentioning

confidence: 99%

Human papillomavirus genotyping after denaturation of specimens for Hybrid Capture 2 testing: Feasibility study for the HPV persistence and progression cohort

LaMere

Kornegay

Fetterman

et al. 2007

Journal of Virological Methods

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Human papillomavirus (HPV) genotyping could be clinically useful, depending on the results of large, prospective studies like the HPV Persistence and Progression cohort. The cohort is based on genotyping and follow-up of Hybrid Capture-positive women at Kaiser Permanente, Northern California. HPV DNA testing by Hybrid Capture 2 requires denaturation with alkali, possibly damaging the DNA for optimal PCR-based genotyping. A feasibility study was conducted on paired aliquots of anonymized specimens from 100 women with low-grade intraepithelial lesion cytology. Test aliquots were left in denaturant for 10 or 18 hours at 4°C and then neutralized; comparison aliquots were not denatured but diluted to match the timing, temperature, concentration and salt conditions of the treated specimens. The masked aliquots were tested using a commercialized PCRbased assay that detects of 37 HPV genotypes. There was no overall effect of treatment on test Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. positivity or number of types. HPV16 was marginally more likely to be detected in untreated versus treated aliquots (P = 0.09) but HPV45 was marginally more likely to be detected in treated than untreated aliquots (P = 0.07), suggesting that these differences represented chance (intra-test variability). It can be concluded that residual Hybrid Capture-positive specimens can be accurately genotyped by PCR after Hybrid Capture 2 processing. NIH Public Access

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Section: Hpv Testingmentioning

confidence: 99%

Human papillomavirus genotyping after denaturation of specimens for Hybrid Capture 2 testing: Feasibility study for the HPV persistence and progression cohort

LaMere

Kornegay

Fetterman

et al. 2007

Journal of Virological Methods

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“…We performed HPV typing on DNA extracted from cells collected in specimen transport medium (Digene, Gaithersburg, MD, USA) using a PCR-based assay employing L1 consensus primers and PGMY09/11 amplification followed by reverse line blot hybridization as described elsewhere (Gravitt et al, 2000;Peyton et al, 2001). Although this PCR method is not necessarily more sensitive analytically than HC2 (Castle et al, 2004), we used the combination of PCR and HC2 results to determine the number of individual HPV types associated with HC2 positive specimens.…”

Section: Hpv Testingmentioning

confidence: 99%

Performance of cytology and human papillomavirus testing in relation to the menstrual cycle

2006

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Cervical smears prepared around the time of menses have been linked to unsatisfactory specimens and false negative results; however, it is unclear whether liquid-based cytology is similarly affected and data relating date of last menstrual period (LMP) to human papillomavirus (HPV) DNA testing are conflicting. Accordingly, we evaluated liquid-based cytology and HPV test results using Hybrid Capture 2 and PCR by LMP (days 0 -10; 11 -21; 22 -28). We studied 5060 participants in ALTS, the Atypical Squamous Cells of Undetermined Significance (ASCUS) Low Grade Squamous Intraepithelial Lesion (LSIL) Triage Study. On average, women had 3.4 examinations (median 4, range 1 -5) during a 2-year period of observation permitting an examination of intra-individual variation in cytology and HPV by LMP. Although uncommon, unsatisfactory cytology specimens were most likely on days 0 -10. For satisfactory specimens, the frequency with which cytologic categories were reported varied by time since LMP, although differences were modest and did not affect the chance of abnormal cytology or its severity among women diagnosed with CIN2 þ . The frequency of positive HC2 tests did not vary with date of LMP. Among HPV infected women, independent of eventual diagnosis and the number of viral genotypes present, mid-cycle specimens yielded the highest frequency of LSIL cytologic interpretations and the highest HPV load; however, the magnitude of these effects were small. Intraindividual correlations of cytology or HPV by LMP were generally weak. We conclude that mid-cycle specimens yield slightly higher HPV DNA loads and slightly increased LSIL interpretations, but the clinical impact is marginal. Standardizing collection times would slightly improve interpretation of trends in HPV load. Finally, these data are consistent with the view that the biological properties of the HPV-infected cervix vary with the date of the LMP. Historically, clinicians have recognized that cytologic samples collected on days of active menstruation are typically bloody and often yield smears that are hypocellular, obscured, and lack endocervical cells (Vooijs et al, 1987). Furthermore, data demonstrating that unsatisfactory cytology specimens are associated with a higher than expected frequency of cervical intraepithelial neoplasia (CIN) and carcinoma (Ransdell et al, 1997;Nygard et al, 2004) in later follow-up, suggest that that these specimens may be linked to false negative results. However, efforts to coordinate return visits to re-screen women with unsatisfactory cytology often fail (McGarahan and Smith-McCune, 2005), and presumably, deferring screening for women who present near the time of menses would present similar problems. Given this dilemma, it is important to determine whether the advantages of liquid-based cytology methods, such as increased cellular recovery and reduction of obscuring by blood (Bernstein et al, 2001), eliminate the association between the performance of cytology and LMP that has been demonstrated for smears.Similarly, the implement...

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“…Several studies reported an association between HPV positivity and lifetime number of sexual partners [16,19,21]. On the other hand, the influence of age at sexual debut and condom use on the risk of being HPV positive is questionable [20,22,23].…”

Section: Introductionmentioning

confidence: 99%

Sociodemographic characteristics, sexual behaviour and knowledge about cervical cancer prevention as risk factors for high-risk human papillomavirus infection in Arkhangelsk, North-West Russia

Roik

Sharashova

Kharkova

et al. 2018

International Journal of Circumpolar Health

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While sociodemographic predictors of cervical cancer (CC) are well understood, predictors of high-risk (HR) human papillomavirus (HPV) infection have not been fully elucidated. This study explored the HR-HPV infection positivity in relation to sociodemographic, sexual behavior characteristics and knowledge about HPV and CC prevention among women who visited the Arkhangelsk clinical maternity hospital named after Samoylova, Russia. This cross-sectional study was conducted in the city of Arkhangelsk, Northwest Russia. Women who consulted a gynecologist for any reason between 1 January 2015 and 30 April 2015 were residents of Arkhangelsk, 25–65 years of age were included. The Mann–Whitney and Pearson’s χ2 tests were used. To determine the HR-HPV status, we used the Amplisens HPV-DNA test. We used a questionnaire to collect the information on sociodemographic factors. Logistic regression was applied. The prevalence of HR-HPV infection was 16.7% (n = 50). HR-HPV infection was more prevalent in younger women, cohabiting, nulliparae, smokers, having had over three sexual partners and early age of sexual debut. The odds of having a positive HR-HPV status increased by 25% with an annual decrease in the age of sexual debut. Moreover women with one child or more were less likely to have positive HR-HPV status.

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Determinants of Genital Human Papillomavirus Detection in a US Population

Cited by 223 publications

References 42 publications

Human papillomavirus genotyping after denaturation of specimens for Hybrid Capture 2 testing: Feasibility study for the HPV persistence and progression cohort

Human papillomavirus genotyping after denaturation of specimens for Hybrid Capture 2 testing: Feasibility study for the HPV persistence and progression cohort

Performance of cytology and human papillomavirus testing in relation to the menstrual cycle

Sociodemographic characteristics, sexual behaviour and knowledge about cervical cancer prevention as risk factors for high-risk human papillomavirus infection in Arkhangelsk, North-West Russia

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