The entrepreneurial interest of students is still relatively low, and the majority of students tend to prefer seeking employment after graduation. However, the number of job opportunities available does not match the number of graduates, highlighting the need for efforts by universities to enhance students' entrepreneurial interest. This is crucial for creating job opportunities and reducing unemployment. This research aims to examine the role of business incubators in strengthening the relationship between entrepreneurial literacy and students' entrepreneurial interest, particularly in Vocational Colleges in Surakarta. The results of this study not only provide insights into the reinforcement of business incubators in the relationship between entrepreneurial literacy and students' entrepreneurial interest but also contribute to understanding this dynamic in Vocational Colleges in Surakarta. This quantitative research utilizes a survey method and was conducted at 8 Vocational Colleges in Surakarta with a sample size of 205 respondents. Data collection techniques included the use of questionnaires. The analysis techniques employed in this research include Moderating Regression Analysis (MRA), F-test, t-test, and coefficient of determination test.