Abstract:RESUMO:Este trabalho apresenta uma modificação dos procedimentos descritos nas Farmacopéias Francesa e Européia para a análise de flavonoides de Passiflora incarnata L., Passifloraceae, por espectrometria UV-Visível e propõe a sua aplicação na determinação dos flavonoides totais das folhas da espinheira-santa (Maytenus aquifolium Mart. e Maytenus ilicifolia (Schrad.) Planch., Celastraceae) e do maracujá (Passiflora edulis Sims. e Passiflora alata Curtis, Passifloraceae). Os resultados obtidos por espectrometri… Show more
“…Mast showed a high content of phenolic compounds in the form of tannins, flavonoids and flavonols in relation to other species of the genus [11,20,21,22,23], strengthening the evidence that flavonoids are the major components in several species of the genus Passiflora (table 1). To corroborate with these observations, it was previously shown that ExEt had a high amount of phenolic compounds and flavonoids using the HPLC analyzis [24].…”
Passiflora cincinnata Mast is a plant popularly used in traditional medicine in northeastern Brazil. The present study aimed to evaluate the anti-lipoperoxidative and anti-α-amylase properties, and cytotoxicity of an extract and fraction of passion fruit leaves (P. cincinnata Mast), as well as the antihyperglecemiant activity of the fraction rich in glycosylated flavonoids and showing low cytotoxicity in rats with a postprandial hyperglycaemia condition. The ethyl acetate fraction (F.ACT) of the P. cincinnata leaves presented the best anti-lipoperoxide properties with TBARs 81.49%, 95.48% and 75.62% lower than AAPH, FeSO4 and H2O2 induced controls, respectively, at 200 μg.mL -1
“…Mast showed a high content of phenolic compounds in the form of tannins, flavonoids and flavonols in relation to other species of the genus [11,20,21,22,23], strengthening the evidence that flavonoids are the major components in several species of the genus Passiflora (table 1). To corroborate with these observations, it was previously shown that ExEt had a high amount of phenolic compounds and flavonoids using the HPLC analyzis [24].…”
Passiflora cincinnata Mast is a plant popularly used in traditional medicine in northeastern Brazil. The present study aimed to evaluate the anti-lipoperoxidative and anti-α-amylase properties, and cytotoxicity of an extract and fraction of passion fruit leaves (P. cincinnata Mast), as well as the antihyperglecemiant activity of the fraction rich in glycosylated flavonoids and showing low cytotoxicity in rats with a postprandial hyperglycaemia condition. The ethyl acetate fraction (F.ACT) of the P. cincinnata leaves presented the best anti-lipoperoxide properties with TBARs 81.49%, 95.48% and 75.62% lower than AAPH, FeSO4 and H2O2 induced controls, respectively, at 200 μg.mL -1
“…Hydrated catechin (Sigma) was used for the standard curve (0.1 to 1.0 mg/mL, R=0.99), and absorbance was read at 500 nm. The test to determine the concentration of flavonoids was based on complexation of these compounds with AlCl 3 (Vetec), as described by Chabariberi et al (2009). Hydrated rutin (Sigma) was used for the standard curve (5 to 50 mg/mL, R= 0.99), and absorbance was read at 425 nm.…”
“…The remaining aqueous extracted material was lyophilized in an Edwards lyophilizer (Brazil) at 0.9 mbar (-40 ºC). The total flavonoids content of each extract (5 and 2.5 mg/mL) compared to a compensation solution were measured spectrophotometrically in triplicate at 427 nm based upon the formation of a complex with 2% AlCl 3 (Pozzi, 2007;Chabariberi et al, 2009). …”
“…Pozzi (2007) and Chabariberi et al (2009) reported on a modification of the spectrometric procedures originally described in the French Pharmacopoeia for the analysis of Passiflora incarnata L., Passifloraceae, flavonoids and proposed its application in the determination of total flavonoids from P. edulis and P. alata leaves. In these studies, the results obtained by a spectrometric procedure were compared to those obtained by high performance liquid chromatography (HPLC-UV).…”
Section: Total Flavonoid Contentmentioning
confidence: 99%
“…In these studies, the results obtained by a spectrometric procedure were compared to those obtained by high performance liquid chromatography (HPLC-UV). The authors demonstrated complete compatibility between the modified French Pharmacopoeia (spectrometric) and HPLC-UV methods (Pozzi, 2007;Chabariberi et al, 2009).…”
Abstract:The Passifloraceae family is extensively used in native Brazilian folk medicine to treat a wide variety of diseases. The problem of flavonoid extraction from Passiflora was treated by application of design of experiments (DOE), as an experiment with mixture including one categorical process variable. The components of the binary mixture were: ethanol (component A) and water (component B); the categorical process variable: extraction method (factor C) was varied at two levels: (+1) maceration and (-1) percolation. ANOVA suggested a cubic model for P. edulis extraction and a quadratic model for P. alata. These results indicate that the proportion of components A and B in the mixture is the main factor involved in significantly increasing flavonoid extraction. In regard to the extraction methods, no important differences were observed, which indicates that these two traditional extraction methods could be effectively used to extract flavonoids from both medicinal plants. The evaluation of antioxidant activity of the extract by ORAC method showed that P. edulis displays twice as much antioxidant activity as P. alata. Considering that maceration is a simple, rapid and environmentally friendly extraction method, in this study, the optimized conditions for flavonoid extraction from these Passiflora species is maceration with 75% ethanol for P. edulis and 50% ethanol for P. alata.
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