Abstract:Recebido em 7/4/05; aceito em 4/11/05; publicado na web em 31/3/06 DETERMINATION OF NITRITE IN WATER USING A FLOWER EXTRACT. Aspects of visible spectrophotometry can be presented to students using simple experiments in which the color of the crude extract of Macroptilium lathyroides (L.) Urb. is bleached in the presence of nitrite ions in acidic medium. The dependence of the absorption intensity with time, the reaction completeness and the Beer law can be demonstrated. Quantitative results for mineral water sa… Show more
“…Macroptilium lathyroides, popularly known as phasey bean, (Ramos, 2006) or wild bean, originates from the tropical part of South America and was introduced in tropical and subtropical India, Australia, Africa and Southeast North America (Ferreira, 2002). It is characterized as a legume with great nitrogen fixing capacity, and can be used as green fertilizer (Lorenzi, 2000); In several regions of Brazil it is used as forage for grazing (Vasconcelos et al, 2011).…”
The monitoring of phytochemicals with potentially toxic properties can be assessed by observing the physiological and cellular alterations of the test organism exposed. This present study aimed to evaluate the cytotoxic and genotoxic potential of aqueous extracts of leaves and roots of Macroptilium lathyroides weed on the cell cycle of lettuce. Bioassays were conducted in a germinator (25 oC) with aqueous extract at concentrations of 0, 5, 10, 20 and 40% p v-1, obtained from fresh leaves and roots. For biological, lettuce root meristems were used for the preparation of slides using the technique of squashing. All blades were observed with an optical microscope at a magnitude of 400x. A total of 5,000 cells were analyzed for each treatment, and the number of cells in each phase of mitosis was recorded. Possible presence of chromosomal abnormalities was verified, such as chromatid breaks, anafasic bridges, loss of whole chromosomes or micronuclei formation. These analyzes were conducted only on a qualitative level. Results showed that aqueous extracts of M. lathyroides mitotic caused reduced index with increased concentration. Genotoxic activity was also observed for both extracts tested, since composition resulted in cell cycle changes and chromosomal abnormalities.
“…Macroptilium lathyroides, popularly known as phasey bean, (Ramos, 2006) or wild bean, originates from the tropical part of South America and was introduced in tropical and subtropical India, Australia, Africa and Southeast North America (Ferreira, 2002). It is characterized as a legume with great nitrogen fixing capacity, and can be used as green fertilizer (Lorenzi, 2000); In several regions of Brazil it is used as forage for grazing (Vasconcelos et al, 2011).…”
The monitoring of phytochemicals with potentially toxic properties can be assessed by observing the physiological and cellular alterations of the test organism exposed. This present study aimed to evaluate the cytotoxic and genotoxic potential of aqueous extracts of leaves and roots of Macroptilium lathyroides weed on the cell cycle of lettuce. Bioassays were conducted in a germinator (25 oC) with aqueous extract at concentrations of 0, 5, 10, 20 and 40% p v-1, obtained from fresh leaves and roots. For biological, lettuce root meristems were used for the preparation of slides using the technique of squashing. All blades were observed with an optical microscope at a magnitude of 400x. A total of 5,000 cells were analyzed for each treatment, and the number of cells in each phase of mitosis was recorded. Possible presence of chromosomal abnormalities was verified, such as chromatid breaks, anafasic bridges, loss of whole chromosomes or micronuclei formation. These analyzes were conducted only on a qualitative level. Results showed that aqueous extracts of M. lathyroides mitotic caused reduced index with increased concentration. Genotoxic activity was also observed for both extracts tested, since composition resulted in cell cycle changes and chromosomal abnormalities.
“…Macroptilium lathyroides é uma planta daninha popularmente conhecida como feijão-de-porco ou feijão selvagem (RAMOS, 2006). Caracteriza-se por ser uma espécie leguminosa com grande capacidade de fixação de nitrogênio, podendo ser utilizada até como adubo verde (LORENZI, 2000;VASCONCELOS et al, 2011).…”
Resumo: A alelopatia caracteriza-se por efeitos na germinação ou no desenvolvimento das plantas, causados por substâncias químicas que são produzidas e liberadas por outras plantas. O presente trabalho teve como objetivo verificar o potencial alelopático do extrato aquoso de material vegetal fresco das folhas e raízes de Macroptilium lathyroides na germinação e no desenvolvimento inicial de plântulas de alface. Foram avaliadas concentrações na proporção peso/ volume (p.v-¹) 0, 5, 10, 20 e 40% dos extratos das folhas e raízes. O experimento foi organizado em esquema fatorial 2 x 5-o extrato de 2 partes da planta e 5 concentrações-, com 4 repetições de 25 sementes. As sementes foram mantidas em caixas gerbox sobre papéis umedecidos com os extratos das folhas e raízes. Diariamente foram realizadas avaliações de germinação e índice de velocidade de germinação (IVG) e, no fim de dez dias, de comprimento da parte aérea, comprimento radicular e massa seca de plântula. Os resultados demonstraram que os extratos de folhas e raízes dessa espécie possuem potencial alelopático, provocando interferência na germinação e no crescimento de alface, sendo que a ação inibitória exercida pelos extratos das folhas foi mais drástica sobre as variáveis analisadas. Palavras-chave: aleloquímicos; Lactuca sativa; inibição; plantas daninhas. Allelopathic potential of aqueous extract of fresh leaves and roots of Macroptilium lathyroides on germination and early growth of lettuce Abstract: Allelopathy is characterized by effects on plant development, caused by chemical substances produced and released by other plants. This study aimed at verifying the allelopathic potential of aqueous extract of fresh plant material from leaves and roots of Macroptilium lathyroides on germination and early development of lettuce. Concentrations were evaluated in weight / volume 0, 5, 10, 20 and in 40% of the extracts of leaves and roots. The experiment was organized in a 2x5 factorial scheme, with two parts of the plant and concentrations with four replicates of 25 seeds. The seeds were kept in gerboxes germibox on moistened paper with extracts of leaves and roots daily. Reviews of germination, IVG and, by the end of ten days, shoot length, root length and plantlet dry weight were performed. The results showed that the extracts of leaves and roots of this species present allelopathic effects, interfering on lettuce's germination and growth, and the inhibitory action exerted by the leaf extracts were analyzed on more drastic variables.
“…A síndrome do bebê-azul ocorre porque o nitrito oxida os íons ferrosos da hemoglobina a íons férricos, gerando a meta-hemoglobina, que é menos eficiente na absorção e transferência de oxigênio para as células e favorece a formação potencial de nitrosaminas e nitrosamidas carcinogênicas, dois efeitos adversos à saú-de, potencializadores do aparecimento de células cancerígenas (Alaburda & Nishihara, 1998;Ramos et al, 2006).…”
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.