Abstract:Recebido em 23/01/2019; aceito em 06/05/2019; publicado na web em 27/06/2019 DETERMINATION OF DESIGNER DRUGS IN SALIVA BY PAPER SPRAY MASS SPECTROMETRY. Determination of two drugs of abuse in saliva has been performed by Paper Spray Ionization Mass Spectrometry (PSI-MS) using a Molecularly Imprinted Polymer (MIP) coated paper as substrate. The method has been optimized for quantification of 3,4-methylenedioxy-N-methylamphetamine (MDMA) and methamphetamine, 3,4-methylenedioxyamphetamine (MDA) from synthetic sal… Show more
“…Thus, it is necessary to develop analytical methods that prioritize the resolution of these problems. With Ambient Mass Spectrometry (AMS) 8 , and the introduction of techniques such as: direct Analysis in Real Time (DART) and Desorption Electrospray Ionization (DESI), there is a new way to generate ions in MS 7 , 9 . AMS is characterized by ion generation under ambient conditions and direct compound analysis in its native environment with minimal or no sample preparation 10 .…”
Fibromyalgia is a rheumatological disorder that causes chronic pain and other symptomatic conditions such as depression and anxiety. Despite its relevance, the disease still presents a complex diagnosis where the doctor needs to have a correct clinical interpretation of the symptoms. In this context, it is valid to study tools that assist in the screening of this disease, using chemical work techniques such as mass spectroscopy. In this study, an analytical method is proposed to detect individuals with fibromyalgia (n = 20, 10 control samples and 10 samples with fibromyalgia) from blood plasma samples analyzed by mass spectrometry with paper spray ionization and subsequent multivariate classification of the spectral data (unsupervised and supervised), in addition to the treatment of selected variables with possible associations with metabolomics. Exploratory analysis with principal component analysis (PCA) and supervised analysis with successive projections algorithm with linear discriminant analysis (SPA-LDA) showed satisfactory results with 100% accuracy for sample prediction in both groups. This demonstrates that this combination of techniques can be used as a simple, reliable and fast tool in the development of clinical diagnosis of Fibromyalgia.
“…Thus, it is necessary to develop analytical methods that prioritize the resolution of these problems. With Ambient Mass Spectrometry (AMS) 8 , and the introduction of techniques such as: direct Analysis in Real Time (DART) and Desorption Electrospray Ionization (DESI), there is a new way to generate ions in MS 7 , 9 . AMS is characterized by ion generation under ambient conditions and direct compound analysis in its native environment with minimal or no sample preparation 10 .…”
Fibromyalgia is a rheumatological disorder that causes chronic pain and other symptomatic conditions such as depression and anxiety. Despite its relevance, the disease still presents a complex diagnosis where the doctor needs to have a correct clinical interpretation of the symptoms. In this context, it is valid to study tools that assist in the screening of this disease, using chemical work techniques such as mass spectroscopy. In this study, an analytical method is proposed to detect individuals with fibromyalgia (n = 20, 10 control samples and 10 samples with fibromyalgia) from blood plasma samples analyzed by mass spectrometry with paper spray ionization and subsequent multivariate classification of the spectral data (unsupervised and supervised), in addition to the treatment of selected variables with possible associations with metabolomics. Exploratory analysis with principal component analysis (PCA) and supervised analysis with successive projections algorithm with linear discriminant analysis (SPA-LDA) showed satisfactory results with 100% accuracy for sample prediction in both groups. This demonstrates that this combination of techniques can be used as a simple, reliable and fast tool in the development of clinical diagnosis of Fibromyalgia.
“…A técnica de ionização por paper spray vem sendo aplicada com sucesso em análises rápidas em diversas matrizes biológicas como urina, sangue total, saliva, tecidos, dried blood spots e dried urine spots (MANICKE et al, 2011;WANG et al, 2011;ZHANG et al, 2012;YANG et al, 2012;SU et al, 2013;KLAMPFL & HIMMELSBACH, 2015;VEGA et al, 2016;MAURER, 2017c;YANNELL et al, 2017;KEATING et al, 2018;MACIEL et al, 2019). O PS-MS já foi utilizado em análises de fármacos terapêuticos e seus respectivos produtos de biotransformação em DBS, na qual os analitos foram extraídos da matriz pelo solvente de pulverização (ZHANG et al, 2012;OUYANG, 2018).…”
LINHARES, A. L. F. A. Detection of anabolic androgenic steroids and its biotransformation products in dried urine spots by paper spray-mass spectrometry ionization (PS-MS). 2020. 142f. Dissertação (Mestrado em Farmácia -Fisiopatologia e Toxicologia) -
“…When a high voltage is applied to the paper, a spray with charged droplets is generated at the paper tip and goes toward the mass spectrometer inlet for MS analysis . The analytical performance of PSI-MS has been evaluated in a wide range of applications, including the detection of drugs in fingerprint samples, quantifications of pesticides in fruits and vegetables, , characterization of energetic polymers, investigations of inks and questioned documents, analysis of drugs of abuse, , and coupling to portable mass spectrometers for on-site analysis . In addition, PSI-MS has been widely employed in clinical diagnosis, − because of its ability to allow biofluid analysis using a small amount of sample with minimal or even no sample preparation (for example, by applying few microliters of plasma, serum, or whole blood directly onto a triangular paper), which can significantly reduce the invasiveness to the patients and waiting time for clinical reports.…”
Squamous intraepithelial lesion is an abnormal growth of epithelial cells on the surface of the cervix that may lead to cervical cancer. Analytical protocols for the determination of squamous intraepithelial lesions are highly demanded since cervical cancer is the fourth most diagnosed cancer among women in the world. Here, paper spray ionization mass spectrometry (PSI-MS) is used to distinguish between healthy (negative for intraepithelial lesion or malignancy) and diseased (high-grade squamous intraepithelial lesion) blood plasmas. A total of 86 blood samples of different women (healthy = 49 samples; diseased = 37 samples) were collected, and the plasmas were prepared. Then, 10 µL of each plasma sample was deposited onto triangular papers for PSI-MS analysis. No additional step of sample preparation was necessary. The interval-successive projection algorithm linear discriminant analysis (iSPA-LDA) was applied to the PSI mass spectra, showing six ions (mostly phospholipids) that were predictive of healthy and diseased plasmas. Values of 77% of accuracy, 86% of sensitivity, 80% of PPV, and 75% of NPV were achieved. This study provides evidence that PSI-MS may potentially be used as a fast and simple analytical technique for early diagnosis of cervical cancer.
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