DETEKSI POLYMORPHISME DENGAN SUBSTITUSI NUKLEOTIDA TUNGGAL PADA Streptococcus agalactiae ISOLAT LOKAL INDONESIA

Lusiastuti, Angela Mariana; Seeger, H.; Sugiani, Desy; Mufidah, Tatik; Novita, Hessy

doi:10.15578/ma.10.2.2015.91-95

Cited by 3 publications

(3 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…β-hemolytic type bacteria have different characteristics when cultured on brain heart infusion agar media, namely, thick colonies, rather light-colored, slimy, and easy to harvest, whereas non-hemolytic type bacteria tend to be rather thin, transparent in color, sticky, and difficult to harvest. Studies on streptococcal on tilapia culture at the Cirata Reservoir in West Java, Indonesia, which were characterized using multilocus sequence typing (MLST), identified non-hemolytic S. agalactiae as the causative agent [63]. The results of this research prove that S. agalactiae in fish belongs to GBS, which is categorized as dangerous to humans.…”

Section: S Agalactiae Casementioning

confidence: 79%

See 1 more Smart Citation

Impact of temperature on the virulence of Streptococcus agalactiae in Indonesian aquaculture: A better vaccine design is required

Lusiastuti,

Suhermanto,

Hastilestari

et al. 2024

Vet World

View full text Add to dashboard Cite

Due to their poikilothermic nature, fish are very sensitive to changes in temperature. Due to climate change, the average global temperature has increased by 1.5°C in the last century, which may have caused an increase in farmed fish mortality recently. Predictions using the model estimate that a 1°C increase in temperature could cause 3%-4% and 4%-6% mortality due to infectious diseases in organisms living in warm and temperate waters, respectively. There is a need to determine whether there is a relationship between increasing environmental temperature and disease virulence. This review examines the influence and impact of increasing temperatures due to climate change on the physiology and pathogenicity of Streptococcus agalactiae, which causes streptococcosis in tilapia and causes significant economic losses. Changes in the pathogenicity of S. agalactiae, especially its virulence properties due to increasing temperature, require changes in the composition design of the fish vaccine formula to provide better protection through the production of protective antibodies. Keywords: adaptation, microbes, pathogen, temperature, virulence.

show abstract

Section: S Agalactiae Casementioning

confidence: 79%

“…S. agalactiae is the main cause of death in tilapia culture worldwide, particularly in Indonesia [63]. Tilapia streptococcal disease was originally caused by GBS, a virulent disease that also affects cattle and humans.…”

Section: S Agalactiae Casementioning

confidence: 99%

Impact of temperature on the virulence of Streptococcus agalactiae in Indonesian aquaculture: A better vaccine design is required

Lusiastuti,

Suhermanto,

Hastilestari

et al. 2024

Vet World

View full text Add to dashboard Cite

show abstract

“…The total base pair of each treatment was 167 bp, even the negative control has nucleotide base difference with all treatments which infected with WFS (8.4% in A, 13% in B, 13% in C and 15% in positive control). There was polymorphism (mutation) in CypA gene because two different DNA sequences had changed more than 1% population (Lemey, Rambaut, Drummond, & Suchard, 2009; Lusiastuti, Seeger, Sugiani, Mufidah, & Novita, 2015). In this study, the polymorphism of whiteleg shrimp CypA gene is an attempt to survive against WFS.…”

Section: Discussionmentioning

confidence: 99%

Curative impacts of squid (Loligosp.) ink extract on haemocyte, digestive enzymes andCypAgene expression of Vaname Shrimp (Litopenaeus vannamei) against white faeces syndrome (WFS)

et al. 2020

View full text Add to dashboard Cite

Right now, high economical value shrimp species is vaname shrimp (Litopenaeus vannamei). Salinity gives impact to shrimp live, immunity and health. Stress from salinity shock will reduce shrimp health and production because of pathogen attack. White faeces syndrome (WFS) is a shrimp disease which frequently occured in an intensive farm. Fighting against WFS has been done with several methods, among other things by using ink extract as metabolite product of squid (Loligo sp.) because of its antibacterial content. To solve the problem, combination of ink extract with salinity treatments to CypA gene expression THC, DHC and digestibility of vaname shrimp (L. vannamei) was investigated. The best treatment using squid ink extract was 27 ppt salinity for vaname shrimp (L. vannamei) against WFS, because it gave the highest SR (85.73%), increased protease until 378 × 10 −3 U/ml, amylase until 4.029 × 10 −3 unit/ml and lipase until 3.613 × 10 −3 unit/ml. and also non-specific immune, i.e. THC 11.89 × 10 6 cells/ml and DHC (hyaline was 39.09%, granular cell was 21.43% and semi-granular cell was 47.74%), while mutation (>1%) was happened in CypA gene.

show abstract

Der p 1 gene sequence polymorphism in house dust mite Dermatophagoides pteronyssinus

et al. 2020

View full text Add to dashboard Cite

Abstract. Adji A, Niode NJ, Memah VV, Posangi J, Wahongan GJP, Tallei TE. 2021. Der p 1 gene sequence polymorphism in house dust mite Dermatophagoides pteronyssinus. Biodiversitas 22: 72-78. Gene polymorphisms in Dermatophagoides pteronyssinus (DP) mite can affect the immune response that plays a role in atopic disease. This study aimed to determine the polymorphisms of Der p 1 gene from DP. House dust containing DP was used as a source of DNA containing the Der p 1 gene. The gene was amplified using specific primers DP Der 1 P 108F and DP Der 1 P 1509R. The amplified fragments were cloned using the Toyobo pTA2 vector and sorted then compared with the Gene Bank database. The result shows that there are 8 polymorphism sites in the Der p 1 gene positioned at 190 (GR), 463 (CT), 645 (AR), 751 (CY, CT), 787 (CG), 827 (TA; TW), and 1084 (CG). In the Der p 1 gene, there are 5 silent mutations and 3 nonsynonymous substitutions, resulting in amino acid polymorphisms as follows: Histidine H146 to Tyrosine Y146, Alanine A220 to Valine V220 and amino acid X220, and Tyrosine T232 to Serine S232. Polymorphisms in these amino acids lead to a polarity change, from polar to polar and non-polar to non-polar. These dynamics in polarity does not change the structure or function of the allergen protein. So that they can be used for vaccine design strategies.

show abstract

DETEKSI POLYMORPHISME DENGAN SUBSTITUSI NUKLEOTIDA TUNGGAL PADA Streptococcus agalactiae ISOLAT LOKAL INDONESIA

Cited by 3 publications

References 3 publications

Impact of temperature on the virulence of Streptococcus agalactiae in Indonesian aquaculture: A better vaccine design is required

Impact of temperature on the virulence of Streptococcus agalactiae in Indonesian aquaculture: A better vaccine design is required

Curative impacts of squid (Loligosp.) ink extract on haemocyte, digestive enzymes andCypAgene expression of Vaname Shrimp (Litopenaeus vannamei) against white faeces syndrome (WFS)

Der p 1 gene sequence polymorphism in house dust mite Dermatophagoides pteronyssinus

Contact Info

Product

Resources

About