Deteksi Keberadaan Citrus Vein Phloem Degeneration (Cvpd) Dengan Teknik PCR (Polymerase Chain Reaction) Di Sulawesi Tenggara

Abstract

Detection of ocurrence Citrus Vein Phloem Degeneration (CVPD) with PCR (Polymerase Chain Reaction) technique in Southeast Sulawesi. CVPD disease incidence in Southeast Sulawesi based on symptoms have never been reported. PCR (polymerase chain reaction) technique was utilized to detect CVPD with specific primer forward OI and reverse OI2, specific for amplication of Liberobacter asiaticum DNA. The result of the reseach indicated that 69% of orange trees in Ladongi, Kolaka District and 65% of orange trees in Lalembuu 1, Konawe Selatan district showed the symptom. PCR technique successfully amplified DNA of bacterium L. asiaticum with the size of 1100 bp.

Relationship Between whitefly (Bemisia tabaci) Population and Pepper Yellow Leaf Curl Disease on Chili Plant Yield in The Field

“…Kejadian penyakit kuning keriting pada pertanaman cabai dapat mencapai 100% di Sulawesi Tenggara (Taufik, 2021) dan kehilangan hasil dapat mencapai 20-100% (Sulandari, 2006;Setiawati et al, 2008). Hal ini disebabkan karena kerusakan yang ditimbulkannya pada tanaman cabai mulai dari ringanmosaik ringan, berat-daun malformasi dan kerdil sehingga tanaman tidak menghasilkan buah cabai (Rusli et al, 2012;Sudiono et al, 2005;Taufik, 2021).…”