Detection, Subgroup Specificity, and Genotype Diversity of Rotavirus Strains in Children with Acute Diarrhea in Paraguay

Coluchi, Norma; Munford, Veridiana; Manzur, Julio; Vázquez, Cynthia; Escobar, Mabel; Weber, Ernesto; Marmol, P. del; Rácz, Maria Lucia

doi:10.1128/jcm.40.5.1709-1714.2002

Cited by 71 publications

(90 citation statements)

References 28 publications

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“…During the present study, all the rotavirus isolates from infants/children showed a typical migration of dsRNA segments in 4:2:3:2 pattern, which is indicative of Group A rotaviruses (Fig.1). Similar pattern of group A rotavirus have been reported from Paraguay 8 , Botswana 9 and other places among humans. Rotaviruses are further divided into two main electropherotypes as "long electropherotype" and "short electropherotype" based on the movement of their genomic segment 10 and 11 10 .…”

Section: Resultssupporting

confidence: 55%

Detection of human rotavirus in hospitalized diarrheic children in central India

et al. 2007

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During the present study, group A human rotaviruses were detected among diarrheic children using polyacrylamide gel electrophoresis (PAGE) technique, with a typical RNA migration pattern of 4:2:3:2, suggestive of group A rotavirus. During the study, a total of 46 fecal samples collected from hospitalized children with acute diarrhea as well as children inhabiting nearby animal farms with history of presence of animal rotaviruses on the farms were processed for detection of human rotavirus. Out of 33 diarrheic children, 12 showed presence of rotavirus infection (36.36%), however, none of the children from animal farm areas showed presence of rotavirus. Female children were more susceptible to rotavirus infection (46.15%) than males (30%). Majority of the cases of rotavirus gastroenteritis belonged up to one year of the age, with an incidence of 40.91%. RNA profi le of rotaviruses suggested circulation of 5 different electropherotypes in this geographical locale of the country, indicating existence of genomic diversity among human rotaviruses. Majority of the isolates were of long pattern (66.67%), whereas short pattern was detected only in one third of the viruses. This preliminary study emphasizes for further detailed studies on the molecular characterization of rotaviruses circulating in this part of country and their relationship with other human rotavirus strains and animal strains in the country.

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Section: Resultssupporting

confidence: 55%

Detection of human rotavirus in hospitalized diarrheic children in central India

et al. 2007

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“…However, G6 strains have been detected in human cases in Italy [Gerna et al, 1992] and Australia [Cooney et al, 2001] and G8 strains have been detected in Indonesia [Matsuno et al, 1985], Finland [Gerna et al, 1990], Italy [Gerna et al, 1990], England [Steele et al, 1999], and Australia [Palombo et al, 2000]. Serotype G10 strains have been detected in human infections in Brazil [Santos et al, 1998], Paraguay [Coluchi et al, 2002], and India [Iturriza-Gómara et al, 2004]. In Africa, the prevalence of G8 strains may be as high as G3 and G4 strains in the rest of the world [Santos and Hoshino, 2005].…”

Section: Introductionmentioning

confidence: 99%

The detection and molecular characterization of human G12 genotypes in South Africa

Page

Beer

Seheri

et al. 2008

Journal of Medical Virology

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The last decade has seen an increase in the detection of rotavirus strains other than G1–G4 emerging or even predominating in some settings. The performance of the current rotavirus vaccines against unusual or rare circulating rotavirus serotypes cannot be predicted and continuous monitoring of wild type rotaviruses will remain a priority. Routine molecular rotavirus surveillance conducted in the Gauteng Province, South Africa during 2004, resulted in the detection of strains that could not typed using standard G specific genotyping primers. Sequencing of the first round amplicons revealed 19 serotype G12P[6] strains and one G12P[8] strain. Phylogenetic analyses of the G12 strains indicated that these strains are probably a recent introduction into South Africa and emerged from a strain related to the Indian isolate ISO‐5. The association of the South African G12s with the P[6] genotype may suggest a mechanism for unusual strains to become more ecologically suited to local population transmission dynamics. This is the first report of serotype G12 strains on the African continent and continued surveillance will be required to track the emergence of G12 strains in Africa. J. Med. Virol. 81:106–113, 2009. © 2008 Wiley‐Liss, Inc.

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“…In some other cases the genetic relatedness determined through nucleotide sequencing has suggested an animal rotavirus as the likely origin of human isolates [17,38,84]. There have been many reports of human rotaviruses with unusual combinations of subgroup, G-P-type, suggesting considerable diversity among rotavirus strains worldwide [25,29,35,36,82]. Moreover, there are also reports of unusual strains such G5, G8 and G10 occurring locally in certain parts of world [25,29,30,36].…”

Section: Animal Strains and The Zoonotic Potential Of Human Rotavirusmentioning

confidence: 99%

“…There have been many reports of human rotaviruses with unusual combinations of subgroup, G-P-type, suggesting considerable diversity among rotavirus strains worldwide [25,29,35,36,82]. Moreover, there are also reports of unusual strains such G5, G8 and G10 occurring locally in certain parts of world [25,29,30,36]. These findings indicate that there is a huge diversity of rotaviruses circulating in humans and, more importantly in the context of public health, animal strains have also been found to be the cause of infections in humans.…”

Section: Animal Strains and The Zoonotic Potential Of Human Rotavirusmentioning

confidence: 99%

Rotavirus Infection: A Perspective on Epidemiology, Genomic Diversity and Vaccine Strategies

Mukherjee

Chawla‐Sarkar

2011

Indian J. Virol.

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Detection, Subgroup Specificity, and Genotype Diversity of Rotavirus Strains in Children with Acute Diarrhea in Paraguay

Cited by 71 publications

References 28 publications

Detection of human rotavirus in hospitalized diarrheic children in central India

Detection of human rotavirus in hospitalized diarrheic children in central India

The detection and molecular characterization of human G12 genotypes in South Africa

Rotavirus Infection: A Perspective on Epidemiology, Genomic Diversity and Vaccine Strategies

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