Detection Staphylococcus aureus Producing Enterotoxin A on the Skewers Meatballs Product in Yogyakarta City Indonesia

Abstract

Meatballs are snacks that are sold by street vendors in the school area and some public places in Yogyakarta city. Based on the materials used and the traditional producing process, it is possible that the hearty snacks can be contaminated by enterotoxin-producing Staphylococcus bacteria. This study aims to detect the presence of enterotoxin-producing Staphylococcus on skewers meatballs. Skewed meatball samples were taken from ten different locations in Yogyakarta city. The samples were grown on the Staphylococcus sp test standard medium, Baird Parker Agar (BPA). The colony candidates were then selected into Mannitol Salt Agar (MSA), carbohydrate fermentation medium, gram staining and then confirmed using the API Staph. Molecular characterization by detecting nuc genes and sea genes as encode enterotoxin A producers. The result of this research showed that the total bacteria ranged from 1.7x104 - 2.0x109 CFU/g. Identification results using API Staph were found as S. aureus, S. cohni, S. xylosus, S. lentus, S. warneri, and S. sciuri while nuc and sea genes were only found in S. aureus.

Detection of mecA Gene and Identification of Potential Methicillin-resistant Staphylococcus Aureus in Hospital Wastewater Samples

“…Baird-parker agar (BPA) medium was chosen for the isolation of Staphylococcus spices in this study because the medium is the recommended selective medium used specially for the identification of Staphylococcus aureus.The results of the growth of colonies of aureus and non-aureusStaphylococcus from the samples of Polgahawela clinical wastewater studied are shown in Figure1. Colonies of S. aureus have a type of the selective medium of BPA[10]. The BPA medium is a differential selective medium for the isolation and detection of Staphylococcus sp and S. aureus bacteria groups.…”