Detection, seroprevalence and antimicrobial resistance of Yersinia enterocolitica and Yersinia pseudotuberculosis in pig tonsils in Northern Italy

Bonardi, S.; Bruini, Ilaria; D’Incau, Mario; Damme, Inge Van; Carniel, Élisabeth; Brémont, Sylvie; Cavallini, Pierugo; Tagliabue, Silvia; Brindani, F.

doi:10.1016/j.ijfoodmicro.2016.07.033

Cited by 52 publications

(44 citation statements)

References 61 publications

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“…Y. enterocolitica is a psychotropic waterborne and foodborne enteropathogen that has the capability to grow at refrigeration temperatures [3]. Contaminated meat, chicken, milk, and cheese with Y. enterocolitica cause a significant health risk for consumers, especially in young children and infants [4,5].…”

Section: Introductionmentioning

confidence: 99%

Yersinia enterocolitica: Prevalence, virulence, and antimicrobial resistance from retail and processed meat in Egypt

2019

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Aim: The objectives of this study were to investigate the prevalence of Yersinia enterocolitica in retail chicken meat, ground and processed beef meat, determine their virulence-associated genes, antimicrobial susceptibility pattern, molecular detection of extended-spectrum β-lactamases, and their capability of biofilm formation in vitro. Materials and Methods: A total of 210 samples (120 retail chicken meat, 30 ground beef, 30 beef burger, and 30 sausage samples) were collected from different retail chicken outlets and markets located at Mansoura city between December 2016 and April 2017. Meat samples were examined bacteriologically for the existence of Y. enterocolitica; bacterial colonies that displayed positive biochemical properties were subjected to polymerase chain reaction targeting 16 rRNA gene. Y. enterocolitica isolates were tested for their susceptibility to six antimicrobial agents using disk diffusion method. Uniplex PCR was used for screening Y. enterocolitica isolates for the presence of two virulence chromosome-associated genes (ail and yst), and β-lactamases (blaTEM and blaSHV). The capability of Y. enterocolitica to form biofilms was detected by tube method. Results: Thirty Y. enterocolitica isolates (14.29%) were recovered including 19 (15.83%) isolates from chicken meat, 3 (10%) from ground beef, 5 (16.67%) from beef burger, and 3 (10%) from sausage samples. Regarding ail gene, it was detected in 6.67% (2/30), while yst gene detected in 20% (6/30) Y. enterocolitica isolates. About 80%, 70%, 63.33%, and 50% of Y. enterocolitica isolates were sensitive to ciprofloxacin, gentamicin, cefotaxime, and streptomycin, respectively, while 83.33% of Y. enterocolitica isolates were resistant to both ampicillin and cephalothin. Interestingly, 21 (70%) isolates had the capability of biofilms formation in vitro. Among the multidrug-resistant (MDR) strains, a significant difference (p<0.05) was found between MDR and biofilm formation. However, biofilm formation was correlated with the resistance of the isolates to β-lactam antimicrobials and the presence of β-lactam-resistant genes. Conclusion: The presence of Y. enterocolitica in chicken meat, ground and processed beef meat represents a significant health risk for meat consumers, which reflects the contamination of slaughterhouses and processing operations, therefore, strict hygienic measures should be applied to minimize carcasses contamination.

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Section: Introductionmentioning

confidence: 99%

Yersinia enterocolitica: Prevalence, virulence, and antimicrobial resistance from retail and processed meat in Egypt

2019

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“…Due to its rarity, the optimal treatment of this disease has not yet been established. Y. pseudotuberculosis is generally susceptible to a wide range of antibiotics ( 20 ), and third-generation cephalosporins or fluoroquinolones are generally given ( 21 ). Based on the management of Yersinia enterocolitica septicemia, it is recommended that treatment be administered for a period of 3 weeks ( 21 ).…”

Section: Discussionmentioning

confidence: 99%

Far East Scarlet-like Fever Masquerading as Adult-onset Kawasaki Disease

et al. 2018

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A previously healthy 31-year-old man was referred to us with refractory septic shock accompanied by bilateral conjunctival congestion and erythema of his right lower limb. Nine days after admission, he had bilateral desquamation of the fingertips, and his presentation satisfied the criteria for Kawasaki disease. A serological examination was positive for Yersinia pseudotuberculosis, and he was diagnosed with Far East scarlet-like fever (FESLF). Interestingly, his 11-month-old baby boy had similar symptoms around the same time, indicating the intrafamilial transmission of the pathogen. We should consider FESLF when we encounter a familial occurrence of systemic manifestations of Kawasaki disease.

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“…enterocolitica were belonged to 1B biotype which was lower than the present study. Other studies were also isolated this biotype from animal and animal food origin [22,23]. Some researchers reported the other pathogenic biotypes in milk [20].…”

Section: Main Textmentioning

confidence: 98%

Evaluating the rate of contamination of raw milk with pathogenic biotypes of Yersinia enterocolitica in the North-East of Iran

Movafagh

Zeinali

Jamshidi

2019

Preprint

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Abstract Objective: This study aimed to evaluate the contamination rate of raw milk to Y. enterocolitica and biotyping of isolated organisms. Results: The results showed 33% of raw milk samples were contaminated with Yersinia enterocolitica. 1A biotype was the predominant one (26%) but two pathogenic biotypes including 1B (6%) and 5 (1%) were also detected. Isolation of potentially pathogenic Y. enterocolitica from raw milk and consequently its presence in dairy products which are made from raw milk is a public health hazard.

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Detection, seroprevalence and antimicrobial resistance of Yersinia enterocolitica and Yersinia pseudotuberculosis in pig tonsils in Northern Italy

Cited by 52 publications

References 61 publications

Yersinia enterocolitica: Prevalence, virulence, and antimicrobial resistance from retail and processed meat in Egypt

Yersinia enterocolitica: Prevalence, virulence, and antimicrobial resistance from retail and processed meat in Egypt

Far East Scarlet-like Fever Masquerading as Adult-onset Kawasaki Disease

Evaluating the rate of contamination of raw milk with pathogenic biotypes of Yersinia enterocolitica in the North-East of Iran

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