Detection, Quantification, and Microbial Risk Assessment of Group A Rotavirus in Rivers from Uruguay

Bortagaray, Viviana; Girardi, Viviane; Pou, Sonia Alejandra; Lizasoain, Andrés; Tort, Luis Fernando López; Spilki, Fernando Rosado; Colina, Rodney; Victoria, Matías

doi:10.1007/s12560-019-09416-x

Cited by 17 publications

(14 citation statements)

References 46 publications

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“…Seasonal variation was found to influence the prevalence of HRVA, indicating that there was a higher prevalence at lower temperatures, which agrees with results of various studies that favored its prevalence in winter months 22 , 57 , 58 . Interestingly, significant HRVA prevalence was noted at the lower temperature range at WNL, which could be attributed to uncovered surface water exposed to various limiting conditions like direct sunlight, UV light, and high temperatures 59 .…”

Section: Discussionsupporting

confidence: 89%

Novel insights of waterborne human rotavirus A in Riyadh (Saudi Arabia) involving G2 predominance and emergence of a thermotolerant sequence

Nour

Hanif

Alanazi

et al. 2021

Sci Rep

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The routine evaluation of water environments is necessary to manage enteric virus-mediated fecal contamination and the possible emergence of novel variants. Here, we detected human rotavirus A (HRVA) circulating in two wastewater treatment plants, two lakes, irrigation water and a wastewater landfill located in Riyadh. VP7-derived surface protein sequences were assessed by phylogenetic analyses and inspection of thermotolerance-mediated secondary structure and seasonal variation. HRVA was most prevalent at An-Nazim wastewater landfill (AN-WWLF; 63.89%). Phylogenetic analyzes revealed the predominance of HRVA G2 lineage for the first time in Saudi Arabia. Moreover, a single HRVA sequence (2B64I-ANLF3/2018) was recovered at 45 °C from AN-WWLF; secondary structure prediction indicated that this sequence was thermotolerant with a high hydrophobicity, an absence of Ramachandran outliers, and a higher content of proline patches on the protein surface. Varied relationships were significantly observed between sampling areas influenced by temperature ranges (p < 0.05). HRVA prevalence was influenced by seasonal variations, favoring moderate temperatures in late autumn and early winter in all locations. However, a significant temperature impact was detected in Wadi-Hanifah Lake (p = 0.01). Our study extends the knowledge of currently circulating HRVA genotypes, and indicates the probable emergence of thermotolerant strains and seasonally mediated HRVA prevalence.

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Section: Discussionsupporting

confidence: 89%

Novel insights of waterborne human rotavirus A in Riyadh (Saudi Arabia) involving G2 predominance and emergence of a thermotolerant sequence

Nour

Hanif

Alanazi

et al. 2021

Sci Rep

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“…In our study, the probability of infection for swimmers was between 0 and 45% which is higher compared with results obtained from a study conducted in the Rurh river that varied between 0.9% and 2.6% for viral gastroenteritis (rotavirus and norovirus enteritis, Giardiasis and Cryptosporidiosis) (Timm et al, 2016), but were similar to the probability of infection observed in a previous study conducted by our group in the Santa Lucia and Uruguay River in Uruguay which were 64% and 68%, respectively (Bortagaray et al, 2020). The probability of infection was calculated based on all the samples regardless of the season of the year which can add bias concerning the seasonality of enteric virus infections.…”

supporting

confidence: 84%

Comparison of the risk of infection of human rotavirus and astrovirus according to fishing and swimming activities at Las Cañas beach, Uruguay

Bortagaray

Gamazo

Castro

et al. 2022

Journal of Applied Microbiology

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Aims:To estimate the risk of human rotavirus (RV) and astrovirus (HAstV) infections for swimmers and fishers at Las Cañas beach, Uruguay. Methods and Results: Surface water samples were collected monthly for 1 year.The dose-response models used were β-Poisson and 1 F 1 hypergeometric for RV and HAstV, respectively. The probabilities of infection were calculated using a kernel density estimate to fitting the data and then sampling from this distribution (Monte Carlo simulation). The probability of RV infection for fishers was between 0 and 65% and for swimmers was between 0 and 50% (<18 years old) and between 0 and 38% (>18 years old). For HAstV, the probability of infection for fishers was between 0% and 45% and for swimmers was between 0 and 38% (<18 years old) and between 0 and 18% (>18 years old). Conclusions:This study suggests that fishers are at higher risk of infection for both viruses compared with swimmers mainly due to higher viral frequency and concentration at the site for fishing activities.

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“…Surface water resources are usually contaminated with wastewater discharge [ 1 , 2 ]. As a result, there continue to be major public health issues due to the contamination of water resources, such as recreational waters, rivers, as well as wastewater treatment plants (WWTPs), by fecal-mediated enteric viruses [ 3 , 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

Human Adenovirus Molecular Characterization in Various Water Environments and Seasonal Impacts in Riyadh, Saudi Arabia

Nour

Hanif

Zakri

et al. 2021

IJERPH

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The regular monitoring of water environments is essential for preventing waterborne virus-mediated contamination and mitigating health concerns. We aimed to detect human adenovirus (HAdV) in the Wadi Hanifah (WH) and Wadi Namar (WN) lakes, King Saud University wastewater treatment plant (KSU-WWTP), Manfouha-WWTP, irrigation water (IW), and AnNazim landfill (ANLF) in Riyadh, Saudi Arabia. HAdV hexon sequences were analyzed against 71 HAdV prototypes and investigated for seasonal influence. ANLF had the highest HAdV prevalence (83.3%). Remarkably, the F species of HAdV, especially serotype 41, predominated. Daily temperature ranges (22–45 °C and 10–33 °C) influenced the significance of the differences between the locations. The most significant relationship of ANLF and IW to WH and KSU-WWTP was found at the high-temperature range (p = 0.001). Meanwhile, WN was most correlated to ANLF at the low-temperature range (p < 0.0001). Seasonal influences on HAdV prevalence were insignificant despite HAdV’s high prevalence in autumn and winter months, favoring low temperatures (high: 22–25 °C, low: 14–17 °C) at five out of six locations. Our study provides insightful information on HAdV prevalence and the circulating strains that can address the knowledge gap in the environmental impacts of viruses and help control viral diseases in public health management.

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Detection, Quantification, and Microbial Risk Assessment of Group A Rotavirus in Rivers from Uruguay

Cited by 17 publications

References 46 publications

Novel insights of waterborne human rotavirus A in Riyadh (Saudi Arabia) involving G2 predominance and emergence of a thermotolerant sequence

Novel insights of waterborne human rotavirus A in Riyadh (Saudi Arabia) involving G2 predominance and emergence of a thermotolerant sequence

Comparison of the risk of infection of human rotavirus and astrovirus according to fishing and swimming activities at Las Cañas beach, Uruguay

Human Adenovirus Molecular Characterization in Various Water Environments and Seasonal Impacts in Riyadh, Saudi Arabia

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