Detection of virulence genes in Malaysian Shigellaspecies by multiplex PCR assay

Thong, Kwai Lin; Hoe, Susan; Puthucheary, S. D.; Yasin, Rohani Md

doi:10.1186/1471-2334-5-8

Cited by 68 publications

(67 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…PFGE seemed to have higher discriminatory power than the plasmid profile analysis, however, there was no correlation between the PFGE patterns and plasmid profiles (Table 4). All isolates were confirmed by PCR to be pathogenic by the presence of the ipaH gene [11,15,21,22].…”

Section: Discussionmentioning

confidence: 99%

Molecular characteristics of Shigella spp. isolated from patients with diarrhoea in a new industrialized area of Thailand

et al. 2006

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Molecular characteristics of Shigella spp. isolated from patients with diarrhoea in a new industrialized area of Thailand

et al. 2006

View full text Add to dashboard Cite

show abstract

“…Las condiciones de amplificación utilizadas son descritas por Lin Thong et al (2005) y Vargas et al (1999). Como control negativo se utilizó agua de grado molecular en lugar de ADN y como control positivo para todos los genes en estudio se empleó la cepa CB-QCA 3349 Shigella flexneri pues este aislado posee cuatro de los cinco genes en estudio: ipaH, ial, set1A y set1B.…”

Section: Identificación Serológica De Las Especies Del Génerounclassified

“…El gen ipaH es considerado uno de los genes más estables y presentes en todas las especies de Shigella en razón que se encuentra tanto en el cromosoma bacteriano como en múltiples copias en ADN plasmidial (Lin Thong et al, 2005) además este gen se encuentra identificado en otras bacterias patógenas como en E. coli enteropatógena, por lo que se lo considera un gen con altas características virulentas (Aranda et al, 2004). En los análisis realizados el gen ipaH fue encontrado en el 91.1 % de los aislados, postulándose como el de mayor presencia dentro de los genes analizados en este estudio.…”

Section: Detección Molecular De Los Genes De Virulencia Enunclassified

See 1 more Smart Citation

Sensibilidad antimicrobiana y detección de genes de virulencia en aislados clínicos de Shigella spp

Villacrés¹,

Alcocer²,

Zurita³

et al. 2017

REMCB

View full text Add to dashboard Cite

RESUMEN.-El género Shigella comprende las especies S. flexneri, S. sonnei, S. boydii y S. dysenteriae, bacterias responsables de la shigelosis. Éste género se caracteriza por la presencia de multirresistencia a antibióticos y una variedad de factores de virulencia que permiten la infección al hospedero. El objetivo de este estudio fue establecer la sensibilidad antimicrobiana y detectar genes de virulencia "Invasion plasmid antigen H", ipaH; "Invasion-associated locus", ial; "Shigella toxin" Stx; "Shigella enterotoxin 1A", set1A; y "Shigella enterotoxin 1B", set1B en aislados clínicos de Shigella spp. Se analizaron 79 aislados obtenidos de Zurita & Zurita Laboratorios y del hospital Vozandes. Mediante serotipificación, se registraron tres especies: S. flexneri (64.6 %), S. sonnei (29.1 %), y S. boydii (6.3 %). La sensibilidad antimicrobiana siguió el método de difusión con disco según las recomendaciones del Clinical and Laboratory Standars Institute (CLSI). La resistencia obtenida fue a tetraciclina (96.20 %), ampicilina (94.9 %), trimetoprima/sulfametoxazol (86.1 %) y cloranfenicol (84.8 %). No se registraron aislados de Shigella con resistencia a ciprofloxacino, azitromicina ni a ceftriaxona. Se determinó la presencia de genes de virulencia por la reacción en cadena de la polimerasa (PCR). Se determinó una alta prevalencia de los genes ipaH (91.1 %) e ial (82.3 %), los genes codificantes de enterotoxina 1 (set1A y set1B) se encontraron en un 35.4 %. Los resultados obtenidos demuestran la existencia de multirresistencia a antibióticos y cepas virulentas.PALABRAS CLAVES: enterotoxinas, genes de virulencia, resistencia antimicrobiana, serogrupos, Shigella spp.ABSTRACT.-The genus Shigella includes S. flexneri, S. sonnei, S. boydii and S. dysenteriae, which are producers of shigelosis. The genus are characterized due to multidrug resistance to antibiotics and a variety of virulence factors that allow the infection to host. The objective of this study was to establish the antibiotic susceptibility and detection of virulence genes in clinical isolates of Shigella spp.: invasion plasmid antigen H (ipaH), invasion-associated locus (ial), Shigella toxin (Stx), Shigella enterotoxin 1A (set1A), and Shigella enterotoxin 1B (set1B). We analyzed 79 strains from "Zurita & Zurita Laboratorios" and "Hospital Vozandes". Three species were identified: S. flexneri (64.6 %), S. sonnei (29.1 %), and S. boydii (6.3 %) by antiserum agglutination. The antimicrobial susceptibility was performed by following the disk diffusion method and recommendations of the Clinical and Laboratory Standards Institute (CLSI). High resistance was observed to tetracycline (96.2 %), ampicillin (94.9 %), trimethoprim/sulfamethoxazole (86.1 %), and chloramphenicol (84.8 %). No resistance was identified to ciprofloxacin, ceftriaxone and azitromicin. The analysis of the presence of virulence genes was performed using the Polymerase Chain Reaction (PCR). A high prevalence of genes ipaH (91.1 %) and ial (82.3 %) was determined, the encoding genes of enterotoxi...

show abstract

“…They are responsible for shigellosis or bacillary dysentery, a disease that causes high fever, neurological disturbances, and mucus-pyohemorrhagic dysentery (3). These bacteria are responsible for morbidity and mortality in high risk populations such as children under five years of age, senior citizens, toddlers in day-care centers, patients in custodial institutions, homosexual men, and those affected by war and famine (4). Shigellosis is characterized by destruction of the colonic epithelium due to the inflammatory response induced upon bacterial invasion of the mucosa (5).…”

Section: Introductionmentioning

confidence: 99%

Epidemiological characterization of resistance and PCR typing of Shigella flexneri and Shigella sonnei strains isolated from bacillary dysentery cases in Southeast Brazil

Penatti¹,

Hollanda²,

Nakazato³

et al. 2007

Braz J Med Biol Res

View full text Add to dashboard Cite

Shigella spp are Gram-negative, anaerobic facultative, non-motile, and non-sporulated bacilli of the Enterobacteriaceae family responsible for "Shigellosis" or bacillary dysentery, an important cause of worldwide morbidity and mortality. However, despite this, there are very few epidemiological studies about this bacterium in Brazil. We studied the antibiotic resistance profiles and the clonal structure of 60 Shigella strains (30 S. flexneri and 30 S. sonnei) isolated from shigellosis cases in different cities within the metropolitan area of Campinas, State of São Paulo, Brazil. We used the following well-characterized molecular techniques: enterobacterial repetitive intergenic consensus, repetitive extragenic palindromic, and double-repetitive element-polymerase chain reaction to characterize the bacteria. Also, the antibiotic resistance of the strains was determined by the diffusion disk method. Many strains of S. flexneri and S. sonnei were found to be multiresistant. S. flexneri strains were resistant to ampicillin in 83.3% of cases, chloramphenicol in 70.0%, streptomycin in 86.7%, sulfamethoxazole in 80.0%, and tetracycline in 80.0%, while a smaller number of strains were resistant to cephalothin (3.3%) and sulfazotrim (10.0%). S. sonnei strains were mainly resistant to sulfamethoxazole (100.0%) and tetracycline (96.7%) and, to a lesser extent, to ampicillin (6.7%) and streptomycin (26.7%). Polymerase chain reaction-based typing supported the existence of specific clones responsible for the shigellosis cases in the different cities and there was evidence of transmission between cities. This clonal structure would probably be the result of selection for virulence and resistance phenotypes. These data indicate that the human sanitary conditions of the cities investigated should be improved.

show abstract

Detection of virulence genes in Malaysian Shigellaspecies by multiplex PCR assay

Cited by 68 publications

References 22 publications

Molecular characteristics of Shigella spp. isolated from patients with diarrhoea in a new industrialized area of Thailand

Molecular characteristics of Shigella spp. isolated from patients with diarrhoea in a new industrialized area of Thailand

Sensibilidad antimicrobiana y detección de genes de virulencia en aislados clínicos de Shigella spp

Epidemiological characterization of resistance and PCR typing of Shigella flexneri and Shigella sonnei strains isolated from bacillary dysentery cases in Southeast Brazil

Contact Info

Product

Resources

About