Detection of virulence factors of Escherichia coli from children by multiplex polymerase chain reaction

Abstract

A total of 161 Escherichia coli (E. coli) strains isolated from children with urinary tract infection (UTI) were analysed for the genes encoding the virulence factors such as pyelonephritis (pap), s fimbriae (sfa), afimbrial adhesin I (afaI), haemolysin (hly), cytotoxic necrotising factor I (cnf I) and aerobactin (aer) by multiplex PCR. Ninety-four E. coli strains were found to carry at least one virulence factor. Therefore, 58.38% of total population was positive for one virulence gene at least. Percentage of genes within the total population for pap, sfa, afaI, hly, cnf I and aer was found as 22.98, 6.21, 9.94, 1.24, 9.94 and 39.75, respectively. Our analysis showed that sfa-pap (p < 0.001); pap-aer, afaI-aer and cnf I-pap (P < 0.05) and hly-sfa (p < 0.01) significantly co-occurred in their respective samples. In the light of these findings, we suggest an important role of pap causing UTI.

Investigation of P Fimbriae Presence in Escherichia coli Strains Isolated from Urine Samples in Human, and Their Antibacterial Resistance

“…In our isolates, 26.3% of papG was detected while 12% of papA was detected. A study from Ankara in Turkey, parallel to our study, revealed that almost 23% E. coli isolates were positive for pap genes (29). In Iran, Mohajeri (30), Farshad and Emamghorashi (31) and Rahdar et al (32) reported the frequencies of pap genes as 20.5%, 30.2%, 57%, respectively.…”

Clonal spread in Eastern Asia of ciprofloxacin-resistant Escherichia coli serogroup O25 strains, and associated virulence factors

“…Identification of virulence genes was performed by PCR amplification procedures as described previously [15,16]. Isolates were analysed according to virulence genes: diarrhoeagenic factors including Shiga toxin-producing (stx1, stx2 and eae), enteropathogenic (eae and bfp), enterotoxigenic (stII and lt), enteroinvasive (virF and ipaH), enteroaggregative (aafII) and diffuse adherent (daaE) genes; and uropathogenic factors including pyelonephritis (pap), s-fimbrial adhesin (sfa), afimbrial adhesin I (afaI), haemolysin (hly), cytotoxic necrotizing factor I (cnfI) and aerobactin (aer).…”

Phenotypic Identification and Phylogenetic Characterization of Uropathogenic Escherichia coli in Symptomatic Pregnant Women With Urinary Tract Infections in South-Western Nigeria

“…Extra-intestinal uropathogenic E. coli strains are defined as E. coli with enhanced ability to cause infections outside the intestinal tract, such as in the bloodstream, cerebrospinal fluid or urinary tract of the host (Diard et al, 2010). Virulence factors associated with ExPEC include: adhesins, toxins (hemolysin and cytotoxic necrotizing factor), siderophores (aerobactin), host defense avoidance mechanisms/polysaccharides coatings (group II capsules and biofilm formation) and uropathogenic-specific protein (usp) (Arisoy et al, 2006;Skjøt-Rasmussen et al, 2011). Antimicrobial agents of various classes are widely used for therapeutic intervention of urinary tract infections caused by UPEC, but it is also used for prophylactic therapy.…”