Clonal spread in Eastern Asia of ciprofloxacin-resistant Escherichia coli serogroup O25 strains, and associated virulence factors

Uchida, Yukiko; Mochimaru, Tomomi; Morokuma, Yuiko; Kiyosuke, Makiko; Fujise, Masako; Eto, Fujiko; Eriguchi, Yoshihiro; Nagasaki, Yoji; Shimono, Nobuyuki; Kang, Dongchon

doi:10.1016/j.ijantimicag.2009.12.012

Cited by 26 publications

(19 citation statements)

References 28 publications

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“…We focused on ESBL-producing bacteria in broilers to improve public health, because of the high incidence of E. coli contamination on the raw chicken meats [14]. Several β-lactamase genes, namely, bla CTX-M-2 , bla CTX-M-14 , bla , bla CTX-M- 15 and bla CTX-M-44 , detected in broilers in this study have already been observed as clinical iso- lates in Japan [12,24,30,31,38,40], and ESBL-producing E. coli strains have also been previously isolated from broilers in Japan [20]. Kojima et al reported the distribution of ESBL-producing E. coli in food-producing animals at a low prevalence level in 2005.…”

Section: Discussionmentioning

confidence: 94%

Prevalence of Extended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae in Food-Producing Animals

Hiroi¹,

Yamazaki²,

Harada³

et al. 2012

The Journal of Veterinary Medical Science

105

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ABSTRACT. To evaluate the diversity of extended-spectrum -lactamases (ESBL) genes among food-producing animals, 48 isolates of ESBL-producing Escherichia coli isolates were obtained from rectal samples of broilers, layers, beef cattle and pigs, at the slaughterhouse level. ESBL-carrying E. coli were isolated from 60.0% of individual broiler rectal samples, 5.9% of layers, 12.5% of beef cattle and 3% of pigs. One ESBL-producing Klebsiella pneumoniae was isolated from a broiler. The ESBL-positive E. coli isolates from broilers harbored various ESBL genes: bla , bla CTX-M-2 , bla CTX-M-14 , bla CTX-M-15 and bla CTX-M-44 . The plasmid DNAs were analyzed by restriction patterns. Homogeneous band patterns were yielded in those of K. pneumoniae and E. coli isolates harboring the bla CTX-M-2 gene from different farms. No genetic relation between the 2 CTX-M-14 ESBL-producing strains was found by pulsed-field gel electrophoresis, although 2 plasmids in these strains, obtained from different broiler farms, were similar to each other. This study provides evidence that the proliferation of CTX-M-producing E. coli is due to the growth of indigenous CTX-M-producing strains and the possible emergence of strains that acquired CTX-M genes by horizontal transfer in different broiler farms. CTX-M-producing coliforms in broilers should be controlled due to the critical importance of cephalosporins and the zoonotic potential of ESBL-producing bacteria.

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Section: Discussionmentioning

confidence: 94%

Prevalence of Extended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae in Food-Producing Animals

Hiroi¹,

Yamazaki²,

Harada³

et al. 2012

The Journal of Veterinary Medical Science

105

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“…The initial descriptions of ST131 emphasized its association with CTX-M-15, but subsequent studies have shown that it is more commonly ESBL negative and FQ resistant (36)(37)(38). Here, ESBLs were produced by 16% of ST131 isolates compared with 3% of non-ST131 isolates, indicating a substantial clonal spread of ESBL-producing E. coli in women of reproductive age in this region of Australia (39).…”

Section: Discussionmentioning

confidence: 97%

Escherichia coli Sequence Type 131 as a Prominent Cause of Antibiotic Resistance among Urinary Escherichia coli Isolates from Reproductive-Age Women

et al. 2013

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dThe recent emergence of multidrug-resistant Escherichia coli sequence type 131 (ST131) has coincided with an increase in general antibiotic resistance of E. coli, suggesting that ST131 has a contributing role in resistance. However, there is little information about the contribution of ST131 to different clinical syndromes or the basis for its impressive emergence and epidemic spread. To investigate this, we studied 953 E. coli isolates from women of reproductive age in the central west region of New South Wales, Australia, including 623 urinary isolates from patients with cystitis (cystitis isolates) (n ‫؍‬ 322) or pyelonephritis (pyelonephritis isolates) (n ‫؍‬ 301) and 330 fecal isolates from healthy controls. The characteristics studied included ST131 clonal group status, resistance to different antibiotics, presence of virulence factor (VF) genes, and biofilm production. As expected, fecal isolates differed significantly from urinary (cystitis and pyelonephritis) isolates in most of the studied characteristics. Antibiotic resistance was significantly more common in ST131 than in non-ST131 isolates. Both antibiotic resistance and ST131 were more common in pyelonephritis than cystitis isolates and least so among fecal isolates. Within each source group, individual VF genes were more prevalent and VF scores were higher for ST131 than for non-ST131 isolates. For ST131 only, the prevalences of most individual VF genes and VF scores were the lowest in the fecal isolates, higher in the cystitis isolates, and highest in the pyelonephritis isolates. Biofilm production was strongly associated with ST131 status and antibiotic resistance. These results clarify the distribution of the ST131 clonal group and its epidemiological associations in our region and suggest that it exhibits both enhanced virulence and increased antibiotic resistance compared with those of other urinary tract infection (UTI) and fecal E. coli isolates from women of reproductive age. U rinary tract infections (UTIs) are among the most common bacterial infections in humans (1). They are usually caused by specialized strains of Escherichia coli termed uropathogenic E. coli (UPEC), which possess distinct virulence factors (VFs) that can allow them to invade and injure a host. The effective management of UPEC is needed to reduce the associated health care costs and social burden.Among urinary E. coli isolates, the prevalence of antibiotic resistance, especially to commonly used agents, such as fluoroquinolones (FQs), has increased substantially in recent years (2, 3, 4). However, although several studies have examined antibiotic resistance in different populations, most have not examined the related clinical and epidemiological associations. A better understanding of the microbiological basis for the emergence of antibiotic resistance among UPEC isolates is needed to guide the efforts that are aimed at interrupting this process.The recent emergence of multidrug-resistant E. coli sequence type 131 (ST131) has contributed to the rising prevalence of re...

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“…Other non-CTX-M ESBL Ambler class A enzymes have also been described in E. coli ST131 isolates. These enzymes include derivatives of the SHV family (mostly SHV-2 and SHV-12) (32,84,87,95,96) and, based on anecdotal evidence, derivatives of the TEM family (TEM-24 and -52) (15,88). The class A carbapenemase KPC-2 has been found in E. coli ST131 isolates from the United States (seven isolates), France (one isolate), Ireland (one isolate), and China, where they recently caused outbreaks (97)(98)(99)(100).…”

Section: Antibiotic Resistancementioning

confidence: 99%

Escherichia coli ST131, an Intriguing Clonal Group

2014

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SUMMARY In 2008, a previously unknown Escherichia coli clonal group, sequence type 131 (ST131), was identified on three continents. Today, ST131 is the predominant E. coli lineage among extraintestinal pathogenic E. coli (ExPEC) isolates worldwide. Retrospective studies have suggested that it may originally have risen to prominence as early as 2003. Unlike other classical group B2 ExPEC isolates, ST131 isolates are commonly reported to produce extended-spectrum β-lactamases, such as CTX-M-15, and almost all are resistant to fluoroquinolones. Moreover, ST131 E. coli isolates are considered to be truly pathogenic, due to the spectrum of infections they cause in both community and hospital settings and the large number of virulence-associated genes they contain. ST131 isolates therefore seem to contradict the widely held view that high levels of antimicrobial resistance are necessarily associated with a fitness cost leading to a decrease in pathogenesis. Six years after the first description of E. coli ST131, this review outlines the principal traits of ST131 clonal group isolates, based on the growing body of published data, and highlights what is currently known and what we need to find out to provide public health authorities with better information to help combat ST131.

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Clonal spread in Eastern Asia of ciprofloxacin-resistant Escherichia coli serogroup O25 strains, and associated virulence factors

Cited by 26 publications

References 28 publications

Prevalence of Extended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae in Food-Producing Animals

Prevalence of Extended-Spectrum β-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae in Food-Producing Animals

Escherichia coli Sequence Type 131 as a Prominent Cause of Antibiotic Resistance among Urinary Escherichia coli Isolates from Reproductive-Age Women

Escherichia coli ST131, an Intriguing Clonal Group

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