Detection of viral antigen following exposure of one‐day‐old chickens to the Holland 52 strain of infectious bronchitis virus

Owen, Robert L.; Cowen, B. S.; Hattel, Arthur L.; Naqi, S. A.; Wilson, Richard A.

doi:10.1080/03079459108418805

Cited by 52 publications

(49 citation statements)

References 17 publications

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“…The performance of the IPA in detecting IBV antigen after inoculation of SPF chickens compares very favourably with IFA (Nakamura et al, 1991;Owen et al, 1991;Janse et al, 1994;Chen et al, 1996). Naqi (1990) showed that IBV could be detected by IPA in the CAM of inoculated eggs within 15 h p.i., with a detection limit of 10 6.2 EID 50 .…”

Section: Detection Of Ibv Antigenmentioning

confidence: 88%

“…Many papers have been published on the duration of IBV infections in the chicken (Hofstad & Yoder, 1966;Doherty, 1967;Colwell & Lukert, 1969;Cook, 1971;Crinion et al, 1971;Jones & Jordan, 1972;Hawkes et al, 1983;Smith et al, 1985;ElHouadfi et al, 1986;Jones & Ambali, 1987;Bumstead et al, 1989;Ambali & Jones, 1990;Otsuki et al, 1990;Nakamura et al, 1991;Owen et al, 1991;Bhattacharjee et al, 1995;Dhinakar Raj & Jones, 1997a;De Wit et al, 1998b). These studies involve a large variety of sampled organs, different virus strains, different doses, etc., and can therefore be expected to present partly conflicting data about the intervals in which the virus can be detected in the various organs.…”

Section: Time Between Start Of Infection and Samplingmentioning

confidence: 99%

“…While VI was positive for at least 2 weeks, IFA detected IBV until 7 days in trachea, from 7 to 10 days in kidney and bursa, and from 3 to 14 days in epithelial cells on the tips of the villi of the ileum and rectum. Owen et al (1991) compared VI, IFA and IPA for detection of H52 for 10 days p.i. of 1-day-old SPF chickens.…”

Section: Detection Of Ibv Antigenmentioning

confidence: 99%

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Detection of infectious bronchitis virus

Wit¹

2000

Avian Pathology

166

128

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The detection methods for infectious bronchitis virus (IBV) are reviewed. Advantages and disadvantages of available techniques of IBV detection by virus isolation, antigen or genome detection, and serology are discussed. Factors of influence on the level of success in detection of IBV after a disease outbreak are discussed, as are the possibilities and dangers of strain classification by protectotyping, serotyping, epitope-typing and genotyping.

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Section: Detection Of Ibv Antigenmentioning

confidence: 88%

Section: Time Between Start Of Infection and Samplingmentioning

confidence: 99%

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Detection of infectious bronchitis virus

Wit¹

2000

Avian Pathology

166

128

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“…La técnica se realizó de acuerdo a Owen et al (17) Protocolo para el aislamiento de IBV en huevos embrionados SPF (libres de patógenos específicos). Brevemente, los sobrenadantes de las muestras de IBV-positivas (determinada por la detección de virus) se filtraron a través de 0.45 μm; 0.1 ml se inoculó en huevos de gallina embrionados libres de patógenos específicos de 9 días de edad (ICA, Bogotá, Colombia) por la vía cavidad alantoidea.…”

Section: Dna Sequencing and Phylogenetic Analysisunclassified

Genotipificación de variantes del virus de bronquitis infecciosa aviar en el departamento del Tolima, Colombia

Cifuentes-Rincón

Lopes

2016

Rev MVZ Córdoba

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Objective. The aim of this study was identify the different genotypes of infectious bronchitis virus (IVB) present in commercial poultry farms from different localities of the Tolima Department, Colombia. Materials and methods. 105 samples of tracheal swabs of poultry of 21 farms were collected. Poultry had been vaccinated against IVB. An screen to identify positive samples and posteriorly the sequencing of the partial region of the S1 subunit and phylogenetic analysis of the isolates with the reference strains, including the vaccine currently used in the country was performed. Results. Poultry all farms had respiratory signs, but only four farms was confirmed the disease. Positive samples of the IBV (HT6, HT9, HT10 and HT11) were pathogenic for embryos 9-days-old. The HT6 sample was grouped in the same cluster that the Massachusetts strains. The HT9 and HT11 samples showed 99% similarity and were grouped genetically distant from the reference strains and other isolated. The HT10 sample showed low similarities with the isolates and reference strains, grouping alone in another cluster. Conclusions. New genotypes are circulating in the Tolima Department, where there is a risk of genetic recombination. Is believed that vaccines used were not providing cross-protection against the new genotypes.Keywords: Characterization, S1 gene, isolation, purification (Source:DeCS). RESUMENObjetivo. El objetivo de este estudio fue identificar los diferentes genotipos del virus de bronquitis infecciosa (VBI) presente en granjas avícolas comerciales de diferentes localidades del departamento de Tolima, Colombia. Materiales y métodos. Se recolectaron 105 muestras de hisopados traqueales provenientes de aves de 21 granjas. Las aves habían sido vacunadas contra el VBI. Se realizó un "screen" para identificar muestras positivas y posteriormente la secuenciación de la región parcial de la subunidad S1 y el análisis filogenético de los aislamientos con las cepas de referencia, incluidas las vacunas utilizadas actualmente en el país. Resultados. Aves de todas las granjas tenían signos respiratorios, pero sólo cuatro granjas confirmaron la enfermedad. Las muestras positivas de VBI (HT6, Genotyping of news variants of the avian infectious bronchitis virus from Tolima department, ColombiaGenotipificación de variantes del virus de bronquitis infecciosa aviar en el departamento del Tolima, Colombia

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“…Disadvantages of IFA (compared to virus isolation) are its usually lower sensitivity (Wizigmann et al, 1981;Hilbink et al, 1982;Owen et al, 1991), the occurrence of non-specific reactions (Braune et al, 1965;Yagyu et al, 1990) and the lack of serotype specificity (Lukert, 1969;Lucio & Hitchner, 1970;Yagyu et al, 1990). These disadvantages are more pronounced in field samples than in samples from experimentally infected birds held in isolated conditions.…”

Section: Introductionmentioning

confidence: 98%

Detection by immunofluorescent assay of serotype‐specific and group‐specific antigens of infectious bronchitis virus in tracheas of broilers with respiratory problems

Wit

Koch²,

Kant³

et al. 1995

Avian Pathology

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SUMMARYThe performances of seven immunofluorescent assays (IFAs) for infectious bronchitis virus (IBV) were examined on 115 trachea samples collected from 60 broiler flocks with clinical respiratory distress. Whether IBV strains could be serotyped directly on trachea sections by IFAs was examined using four different serotypespecific monoclonal antibodies (MAbs). Two group-specific IFAs using two different group-specific MAbs, were compared with a conventional IFA using a chicken hyperimmune anti-IBV serum. The use of the six MAbs in the IFA showed, in contrast to the use of the hyperimmune serum, no or only faint non-specific staining. Although the sensitivities of the two group-specific IFAs using MAbs were not higher (P> 0.05, power 80%) than the sensitivity of the IFA using hyperimmune serum, the interpretation of the staining of the first two IFAs was easier.Seventeen of the 41 isolated IBV strains could be typed by IFA using the serotypespecific MAbs. Serotyping by IFA was possible in about 70% of the tracheas that stained positive with group-specific MAbs or hyperimmune serum and from which IBV was isolated. Use of serotype-specific IFAs is a new and very fast way of diagnosing IBV infections including serotyping, providing enough time to adjust the vaccination programme for the next broiler flock.

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Detection of viral antigen following exposure of one‐day‐old chickens to the Holland 52 strain of infectious bronchitis virus

Cited by 52 publications

References 17 publications

Detection of infectious bronchitis virus

Detection of infectious bronchitis virus

Genotipificación de variantes del virus de bronquitis infecciosa aviar en el departamento del Tolima, Colombia

Detection by immunofluorescent assay of serotype‐specific and group‐specific antigens of infectious bronchitis virus in tracheas of broilers with respiratory problems

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