Detection of Urinary 8-hydroxydeoxyguanosine (8-OHdG) Levels as a Biomarker of Oxidative DNA Damage among Home Industry Workers Exposed to Chromium

Abstract

Electroplating workers are using chromium during the working process. Clinical and laboratory evidence indicates that exposure of chromium is very toxic if it is inhaled and can lead to oxidative DNA damage. This study was aimed to investigate factors associated to the urinary 8 -OHdG levels as a biomarker of oxidative DNA damage. Sixty six subjects from electroplating home industry in Tegal, Central Java were included. Urinary chromium levels were determined using AAS. The urinary 8-OHdG level as oxidative DNA damage was measured using ELISA. The levels of chromium in all sample were higher than the normal range (median 11.77 μg/ L), the median of urinary 8-OHdG level was 23.83 ng/ml. Eventhough, age and urinary chromium level were not associated with urinary 8-OHdG's levels, there was a significant association between the period of works and the type of jobs to the urinary 8 -OHdG levels.

Health Status and Occupational Health Hazards Among Home-based Garment Workers in Semarang, Indonesia

“…They are usually given raw materials and paid per unit [2]. Workers in home-based industries face risks of accidents and illnesses caused by work attitudes and environmental factors due to a lack of knowledge and safety standards among home-based workers [5].…”

Heavy metals as environmental risk factors for cardiovascular diseases: from the perspective of the renin angiotensin aldosterone system and oxidative stress

“…Toxicity caused by hexavalent Cr was found to be associated with oxidative stress (Velma and Tchounwou, 2013). Reduced Cr reacts with H2O2 to produce •OH ( Table-3) (Setyaningsih et al, 2015), which is capable of attacking DNA bases such as guanine and producing different DNA adducts such as 8-hydroxydeoxyguanosine (8-OH-dG), a reliable biomarker of oxidative stress (Sughis et al, 2012). Previously, Shi et al (1999) also proved that Cr exposure significantly reduces cellular glutathione and NADPH levels ( Table-3).…”

The Role of Green Tea Polyphenols in the Protection from Hexavalent Chromium-Induced Genotoxic Damage

“…Antioxidants such as AscH-could react with •OH, quenching and converting it into a poorly reactive semihydroascorbate radical, which is harmless to the DNA molecule. The C8-OH-adduct radical of deoxyguanosine is formed during catalysis of •OH in the reaction of 2-deoxyguanosine with molecular oxygen, (Figure 3 R-IV); since it induces DNA strand breaks, it is considered a form of oxidative DNA damage [37,38]. Therefore, by activating repair mechanisms, this adduct can be removed through 8-hydroxydeoxyguanosine (8-OHdG, 7,8-dihydro-8-oxodeoxyguanosine), which is a marker repairer of oxidative stress in biological systems that can be measured in fluids such as blood, urine and saliva (Figure 3 R-VII).…”