Detection of undeclared bovine milk in different food matrices by a multi-technique approach

Febo, Tiziana Di; Schirone, Maria; Domenico, Marco Di; Visciano, Pierina; Krasteva, Ivanka; Sonsini, Luigina; Cerquaglia, O.; Carpino, S.; D'Onofrio, Federica; Tittarelli, Manuela; Luciani, Mirella

doi:10.1016/j.idairyj.2020.104845

Cited by 6 publications

(4 citation statements)

References 13 publications

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“…Applications of PCR were used to investigate the adulteration of goat milk produced by smallholders with bovine milk as an adulterant 23 . Several studies have confirmed the potential of DNA-based methods for detecting the fraudulent admixture of milk from different species in milk and milk products 24 , 25 . Nevertheless, each sample must undergo complex and time-consuming preparation procedures, such as DNA isolation and quantification, and the design of species-specific primers 26 .…”

Section: Introductionmentioning

confidence: 94%

Identification of milk from different animal and plant sources by desorption electrospray ionisation high-resolution mass spectrometry (DESI-MS)

et al. 2022

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This study used desorption electrospray ionisation mass spectrometry (DESI-MS) to analyse and detect and classify biomarkers in five different animal and plant sources of milk for the first time. A range of differences in terms of features was observed in the spectra of cow milk, goat milk, camel milk, soya milk, and oat milk. Chemometric modelling was then used to classify the mass spectra data, enabling unique or significant markers for each milk source to be identified. The classification of different milk sources was achieved with a cross-validation percentage rate of 100% through linear discriminate analysis (LDA) with high sensitivity to adulteration (0.1–5% v/v). The DESI-MS results from the milk samples analysed show the methodology to have high classification accuracy, and in the absence of complex sample clean-up which is often associated with authenticity testing, to be a rapid and efficient approach for milk fraud control.

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Section: Introductionmentioning

confidence: 94%

Identification of milk from different animal and plant sources by desorption electrospray ionisation high-resolution mass spectrometry (DESI-MS)

et al. 2022

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“…The procedure of DNA authentication assumes the possibility of detecting partial substitution of the declared type of milk by molecular genetic analysis of unique gene loci (Agrimonti and Marmiroli, 2018;Kalogianni, 2018;Agrimonti et al, 2019). The most common method for species identification in milk and dairy products is PCR followed by electrophoresis and hybridization-fluorescence detection of amplification products (Choopan et al, 2017;Di Domenico et al, 2017;Cosenza et al, 2019;Tsakali et al, 2019;Di Febo et al, 2020;Tsirigoti et al, 2020). Additional authentication approaches based on the length polymorphism of restriction fragments of amplified DNA (PCR-RFLP) have also found their application along with methods of hybridization, sequence, and biosensor analysis of the corresponding nucleotide sequences of mitochondrial and nuclear genes (Abdel-Rahman and Ahmed, 2007;Tillmar et al, 2013;Ripp et al, 2014;Abdelfatah et al, 2015;Beltramo et al, 2017;Kounelli and Kalogianni, Species identification of ruminant milk by genotyping of the κ-casein gene R. R. Vafin, 1 A. G. Galstyan, 2 S. V. Tyulkin, 1 Kh.…”

Section: Introductionmentioning

confidence: 99%

Species identification of ruminant milk by genotyping of the κ-casein gene

Vafin

Genrikhovich

Tyulkin

et al. 2022

Journal of Dairy Science

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“…The cited EU reference analytical methodology No. 273/2008 has several limitations: it is not a high-throughput method, provides only semiquantitative results [16,[33][34][35], and the analysis is time-consuming. Moreover, the method cannot discriminate goat-sheep mixtures [36] and the overlapping of species-specific bands generates complex protein profiles, thus, impairing the interpretation of results [37].…”

Section: Introductionmentioning

confidence: 99%

“…Immunological methods (mainly ELISA) are quite used in the determination of cow's milk in declared sheep, goat, or buffalo products [33,[44][45][46][47][48][49]. The main difficulty lies in the production of antibodies exclusively specific for milk proteins from one species, even if some commercial kits are available.…”

Section: Introductionmentioning

confidence: 99%

Authenticity assessment of dairy products by capillary electrophoresis

et al. 2021

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Authenticity assessment of dairy products by capillary electrophoresisMilk and derivatives are a very important part in the diet of the world population. Products from goat, buffalo, and sheep species have a greater economic value than the cow ones, therefore, authenticity frauds by improperly adding cow's milk occur frequently: dairy products are among the seven more attractive foods for adulteration. Milk from each of the above-cited animal species has its own definite profile of whey proteins (variants of α-lactalbumin and β-lactoglobulin) and its definite profile of caseins (variants of α S1 -, α S2 -, β-, and κ-casein). Such proteins can be usefully exploited as markers of authenticity by using capillary electrophoresis which is the technique of choice for the analysis of proteins. Due to the multiple adjustable parameters that are unknown to other analytical techniques, capillary electrophoresis is able to detect frauds in milk mixtures and cheese with little use of solvents, fast analysis time, and ease of operation. This makes it attractive and competitive for routine checks that are very important to fight the adulteration market. Advantages and limitations are discussed.

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Detection of undeclared bovine milk in different food matrices by a multi-technique approach

Cited by 6 publications

References 13 publications

Identification of milk from different animal and plant sources by desorption electrospray ionisation high-resolution mass spectrometry (DESI-MS)

Identification of milk from different animal and plant sources by desorption electrospray ionisation high-resolution mass spectrometry (DESI-MS)

Species identification of ruminant milk by genotyping of the κ-casein gene

Authenticity assessment of dairy products by capillary electrophoresis

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