Phylogenetic analysis of the sequenced segments of the env gene locus of the BLV provirus and the PCR RFLP genotyping strategy proposed in our works that is consistent with phylogenetic classification of this pathogenic agent allowed the taxonomic identification of BLV isolates identified in cattle in Tatarstan (Russian Federation) as representatives of BLV genotypes 4, 7, and 8. Of the 100 isolates identified, 64 were attributed to BLV genotype 4; 28 BLV representatives belong to the cluster of genotype 7; and the remaining 8 samples of the provirus belong to new genotype 8 of the pathogenic agent. The strategy of BLV PCR RFLP genotyping proposed in our work on the basis of five restriction endonucleases (PvuII, SspI, HphI, HaeIII, and BstYI) allowed for correct genotyping identification of the viral pathogen.
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