Detection of Ultratrace Chloramphenicol Residues in Milk and Chicken Muscle Samples Using a Chemiluminescent ELISA

Tao, Xiaoqi; Jiang, Haiyang; Zhu, Jinghui; Niu, Lanlan; Wu, Xiaoping; Shi, Weimin; Wang, Zhanhui; Shen, Jianzhong

doi:10.1080/00032719.2012.673335

Cited by 16 publications

(21 citation statements)

References 15 publications

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“…The coating antigen CAP‐OVA were prepared in our previous research . Preparation of Ag‐MBs: The CAP‐OVA was immobilized on the MBs according to the specific protocol provided by Bi .…”

Section: Methodsmentioning

confidence: 99%

“…The coating antigen CAP-OVA were prepared in our previous research. [20] Preparation of Ag-MBs: The CAP-OVA was immobilized on the MBs according to the specific protocol provided by Bi. [15] Briefly, a suspension of MBs (100mL), in a 1.5 ml Eppendorf tube (EP tube), were separated from the solution on a magnetic rack.…”

Section: Preparation Of Antigen-immobilized Mbs (Ag-mbs)mentioning

confidence: 99%

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An ultrasensitive chemiluminescence immunoassay of chloramphenicol based on gold nanoparticles and magnetic beads

Tao

Jiang

et al. 2013

Drug Testing and Analysis

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A competitive, direct, chemiluminescent immunoassay based on a magnetic beads (MBs) separation and gold nanoparticles (AuNPs) labelling technique to detect chloramphenicol (CAP) has been developed. Horseradish peroxidase (HRP)-labelled anti-CAP monoclonal antibody conjugated with AuNPs and antigen-immobilized MBs were prepared. After optimization parameters of immunocomplex MBs, the IC50 values of chemiluminescence magnetic nanoparticles immunoassay (CL-MBs-nano-immunoassay) were 0.017 µg L(-1) for extract method I and 0.17 µg L(-1) for extract method II. The immunoassay with two extract methods was applied to detect CAP in milk. Comparison of these two extract methods showed that extract method I was advantageous in better sensitivity, in which the sensitivity was 10 times compared to that of extract method II, while extract method II was superior in simple operation, suitable for high throughout screen. The recoveries were 86.7-98.0% (extract method I) and 80.0-103.0% (extract method II), and the coefficients of variation (CVs) were all <15%. The satisfactory recovery with both extract methods and high correlation with traditional ELISA kit in milk system confirmed that the immunomagnetic assay based on AuNPs exhibited promising potential in rapid field screening for trace CAP analysis.

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Section: Methodsmentioning

confidence: 99%

Section: Preparation Of Antigen-immobilized Mbs (Ag-mbs)mentioning

confidence: 99%

An ultrasensitive chemiluminescence immunoassay of chloramphenicol based on gold nanoparticles and magnetic beads

Tao

Jiang

et al. 2013

Drug Testing and Analysis

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“…Furthermore, the chemiluminescent technique, combined with ELISA, was used to detect antibiotic residues. Tao et al 2012developed indirect chemiluminescent enzyme-linked immunoassay (CL-ELISA) for screening CAP in milk and chicken muscle (Tao et al, 2012). Previously, immunoassays, especially the ELISA, have proved to be an efficient method for the analysis of numerous samples in a timely manner.…”

Section: Enzyme-linked Immunosorbent Assaymentioning

confidence: 99%

Current advances in immunoassays for the detection of antibiotics residues: a review

Ahmed

Ning

Peng

et al. 2020

Food and Agricultural Immunology

119

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Food contamination with antibiotic residues has become a worldwide problem due to the heavy and improper use of antibiotics. The development of fast, high-throughput, easy, and economical screening methods for the detection of antibiotic residues is an important requirement as an alternative to the traditional assays. Immunoassays are widespread promising methods that can achieve the conditions of an analytical method in the evaluation of food and environmental protection. This review offers an overview of the preparation of antibodies and the currently established immuneassays for the antibiotic residues detection, including enzymelinked immunosorbent assay, fluorescence immunoassay, radioimmunoassay, colloidal gold immunoassay, and chemiluminescence immunoassay. Furthermore, it will elaborate on the future perspective on the performance and improvement of these assays for the determination of antibiotic residues in food samples. These assays include a simple sample preparation and high-sensitive detection that offer a significant assurance for the screening of antibiotic residues.

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“…A series of buffers such as coating buffer, blocking buffer, washing solution, 0.2 M sodium phosphate buffer (PB, pH 7.2), phosphate-buffered saline (PBS, pH 7.4), assay buffer, and substrate buffer (pH 5.0) were prepared according to the literature (Tao et al 2012;Tao et al 2013). A pH 6.0 buffer was prepared from 0.1 M 2-(N-morpholino) ethanesulfonic acid buffer and 0.5 mol L À1 NaCl.…”

Section: Chemicals and Reagentsmentioning

confidence: 99%

Determination of Salbutamol, Clenbuterol, and Brombuterol in Urine by a Highly Sensitive Chemiluminescence Enzyme Immunoassay

Jiang

et al. 2014

Analytical Letters

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A salbutamol-bovine serum albumin and a salbutamol-ovalbumin coating antigen were synthesized, and six New Zealand white rabbits were treated with the immunogen to obtain polyclonal antibodies to develop a rapid, sensitive, and indirect chemiluminescent enzyme immunoassay (ciCLEIA) for the analysis of swine and bovine urine. The prepared antibodies showed high cross-reactivities with clenbuterol (139.6%) and brombuterol (225%). Under the optimized conditions, the linear dynamic range and the limit of detection (LOD) for salbutamol were from 0.007 to 0.17 lg L À1 and 0.003 lg L À1 , with a correlation coefficient of 0.9965 and a half maximum inhibition concentration of 0.028 lg L À1 . Recoveries for salbutamol, clenbuterol, and brombuterol were from 78.8% to 119.0% with intra-assay and inter-assay coefficients of variation less than 13.9% and 19.7%, respectively. The reported ciCLEIA was about 10-fold more sensitive for salbutamol and 20-fold more sensitive for clenbuterol compared to conventional methods. This study showed that ciCLEIA was a reliable, convenient, and sensitive method for the simultaneous determination of salbutamol, clenbuterol, and brombuterol in swine and bovine urine.

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Detection of Ultratrace Chloramphenicol Residues in Milk and Chicken Muscle Samples Using a Chemiluminescent ELISA

Cited by 16 publications

References 15 publications

An ultrasensitive chemiluminescence immunoassay of chloramphenicol based on gold nanoparticles and magnetic beads

An ultrasensitive chemiluminescence immunoassay of chloramphenicol based on gold nanoparticles and magnetic beads

Current advances in immunoassays for the detection of antibiotics residues: a review

Determination of Salbutamol, Clenbuterol, and Brombuterol in Urine by a Highly Sensitive Chemiluminescence Enzyme Immunoassay

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