Detection of two orchid viruses using quartz crystal microbalance (QCM) immunosensors

Eun, Alvin Jin-Cherng; Huang, Liejun; Chew, Fook Tim; Li, Sam Fong Yau; Wong, Suei Nee

doi:10.1016/s0166-0934(01)00382-2

Cited by 105 publications

(44 citation statements)

References 28 publications

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“…Amounts as low as 1 ng of two orchid viruses could be detected acoustically by immobilizing specific virus antibodies on the sensor surface (Eun et al, 2002b). This detection limit was again comparable to an ELISA, but as before considerably faster.…”

Section: Virusesmentioning

confidence: 72%

A survey of the 2001 to 2005 quartz crystal microbalance biosensor literature: applications of acoustic physics to the analysis of biomolecular interactions

Cooper¹,

Singleton²

2007

J of Molecular Recognition

335

205

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The widespread exploitation of biosensors in the analysis of molecular recognition has its origins in the mid-1990s following the release of commercial systems based on surface plasmon resonance (SPR). More recently, platforms based on piezoelectric acoustic sensors (principally 'bulk acoustic wave' (BAW), 'thickness shear mode' (TSM) sensors or 'quartz crystal microbalances' (QCM)), have been released that are driving the publication of a large number of papers analysing binding specificities, affinities, kinetics and conformational changes associated with a molecular recognition event. This article highlights salient theoretical and practical aspects of the technologies that underpin acoustic analysis, then reviews exemplary papers in key application areas involving small molecular weight ligands, carbohydrates, proteins, nucleic acids, viruses, bacteria, cells and lipidic and polymeric interfaces. Key differentiators between optical and acoustic sensing modalities are also reviewed.

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Section: Virusesmentioning

confidence: 72%

A survey of the 2001 to 2005 quartz crystal microbalance biosensor literature: applications of acoustic physics to the analysis of biomolecular interactions

Cooper¹,

Singleton²

2007

J of Molecular Recognition

335

205

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“…If the analytical signal is too weak to detect target materials, the detection sensitivity can be increased by introducing the signalenhancing step (Kurosawa et al, 2006). QCMI shows high detection sensitivity for biological materials even viruses (Bachelder et al, 2005;Eun et al, 2002;Kleo et al, 2011;Lee and Chang, 2005;Owen et al, 2007;Su et al, 2003;Susmel et al, 2000;Uttenthaler et al, 2001). Because a detection instrument for QCM is potable and QCM coated with virus-specific antibodies to detect plant viruses has long life span, it can be used for on site detection of plant viruses (Becker and Cooper, 2011;Eun et al, 2002).…”

Section: Quartz Crystal Microbalance Immunosensors (Qcmi)mentioning

confidence: 99%

“…Since QCM has proven to be successful in detection of plant viruses, including Cymbidium mosaic virus (CyMV), TMV and Turnip yellow mosaic virus (TYMV), were detected using QCM (Dickert et al, 2004;Eun et al, 2002;Zan et al, 2012).…”

Section: Quartz Crystal Microbalance Immunosensors (Qcmi)mentioning

confidence: 99%

A Review of Detection Methods for the Plant Viruses

Jeong¹,

Ju²,

Noh³

2014

Research in Plant Disease

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The early and accurate detection of plant viruses is an essential component to control those. Because the globalization of trade by free trade agreement (FTA) and the rapid climate change promote the country-tocountry transfer of viruses and their hosts and vectors, diagnosis of viral diseases is getting more important. Because symptoms of viral diseases are not distinct with great variety and are confused with those of abiotic stresses, symptomatic diagnosis may not be appropriate. From the last three decades, enzyme-linked immunosorbent assays (ELISAs), developed based on serological principle, have been widely used. However, ELISAs to detect plant viruses decrease due to some limitations such as availability of antibody for target virus, cost to produce antibody, requirement of large volume of sample, and time to complete ELISAs. Many advanced techniques allow overcoming demerits of ELISAs. Since the polymerase chain reaction (PCR) developed as a technique to amplify target DNA, PCR evolved to many variants with greater sensitivity than ELISAs. Many systems of plant virus detection are reviewed here, which includes immunological-based detection system, PCR techniques, and hybridization-based methods such as microarray. Some of techniques have been used in practical, while some are still under developing to get the level of confidence for actual use.

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“…Therefore, QCM can be applied not only to chemical gas adsorption but also to biomolecular detection. When applied to biomolecular reactions, the QCM technique can detect pathogenic bacteria in food (Park & Kim, 1998) and plant viruses in plant tissue (Eun et al, 2002). Despite the many potential advantages of QCM, relatively few attempts have been made to extend QCM to the measurement of a living cell.…”

Section: Characters Of Qcms From Cell Monitoringmentioning

confidence: 99%