Detection of Trichinella infection in food animals

Nöckler, Karsten; Pozio, Edoardo; Voigt, W. P.; Heidrich, Jana

doi:10.1016/s0304-4017(00)00350-2

Cited by 76 publications

(59 citation statements)

References 29 publications

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“…A number of studies compare the anti-T. spiralis IgG antibody level (seroconversion) with regard to the infective dose or intensity of T. spiralis infection in pig muscles, most frequently in the diaphragm or only in the predilection muscles, whereas in the present study the T. spiralis infection intensity was presented as the mean from 16 different muscles of each pigs. The time of seroconversion is also dependent on a Trichinella species that triggers infection, infected animal species and breed as well as its individual features (Smith 1987, Smith 1988, Smith and Snowdon 1989, Nöckler et al 2000, Nöckler et al 2005. Van der Leek et al (1992) showed that in three groups of pigs, 3 animals per group, inoculated with 5000, 500 and 50 larvae of T. spiralis, specific anti-T. pigs were experimentally infected with 10 000 T. spiralis larvae.…”

Section: Discussionmentioning

confidence: 99%

“…The time needed to develop a specific IgG antibody response in pigs infected with Trichinella is correlated to the infection dose and the intensity of Trichinella infection in the muscles Kapel 2007, Gottstein et al 2009). In addition, the Trichinella species triggering the infection, the species and breed of the host and its individual features also play a role (Smith 1987, Smith 1988, Smith and Snowdon 1989, Nöckler et al 2000, Nöckler et al 2005. All the steps of the ELISA, including production of Trichinella E-S antigens, can be carried out in a laboratory, yet it requires experienced and qualified staff, high-tech laboratory equipment, maintenance of Trichinella strains for diagnostic purposes and sufficiently long time for laboratory analyses.…”

Section: Introductionmentioning

confidence: 99%

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Detection of Experimental Swine Trichinellosis Using Commercial Elisa Test

Gondek¹,

Bień²,

Nowakowski³

2017

Polish Journal of Veterinary Sciences

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The aim of the study carried out on ten young (10-week old) pigs of the native Polish Large White breed experimentally infected with a low dose of 300 invasive muscle larvae (ML) of Trichinella spiralis was intravital detection of trichinellosis using the E-S ELISA test, determination of a variation level of IgG antibodies against excretory-secretory (E-S) antigens of T. spiralis muscle larvae and finally, describing the intensity of T. spiralis larvae infection in selected muscles. The pig sera were collected at 7 and 9 days prior to the experimental infection with T. spiralis and at 9, 14, 20, 23, 25, 27, 30, 33, 37, 41, 46 days post-infection (d.p.i.). The anti-T. spiralis IgG antibodies were detected by a commercial E-S ELISA test (PrioCHECK Trichinella Ab). Average intensity of the T. spiralis infection in the examined muscles of pigs ranged from 1.52 up to 43.09 larvae/g. The studies revealed that the E-S antigen in the ELISA test did not show cross-reaction with the sera of pigs infected with Oesophagostomum spp. The ELISA assay did not recognize trichinellosis in pigs until 27 days after the T. spiralis infection. The anti-T. spiralis IgG antibodies were first detected on day 30 post-infection. A statistically significant increase of IgG antibodies against T. spiralis ML E-S antigens was first observed between days 27-30 (p<0.01) post-infection, and a further significant rise in the antibody level occurred between days 27 and 33 (p<0.01); 30 and 33 (p<0.01); 33 and 37 (p<0.05) following infection.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Detection of Experimental Swine Trichinellosis Using Commercial Elisa Test

Gondek¹,

Bień²,

Nowakowski³

2017

Polish Journal of Veterinary Sciences

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“…Although serological methods for the detection of Trichinella infection are not considered suitable for meat inspection, they are important for the surveillance of the infection and epidemiological investigations in animal populations, particularly in the areas where prevalence is high [13]. Serological methods are also useful for the presumptive or retrospective diagnosis and surveillance of human infection [14][15][16]. However, conventional serodiagnostic methods, such as the immunofl uorescence antibody assay (IFA) and the enzyme-linked immunosorbent assay (ELISA), can yield results that vary due to antigen quality and may differ in the level of sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Characteristic band pattern in western blots for specific detection of anti-Trichinella spiralis antibodies in different host species

Ilić¹,

Gruden-Movsesijan²,

Živojinovic³

et al. 2014

Acta Veterinaria

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Western blot (Wb) is considered to be the gold standard test for Trichinella infection serology, since this method allows specific Trichinella antigens to be distinguished from cross-reactive antigens. This is not the case with widely used antibody assay techniques - indirect immunofluorescence and ELISA - which are sensitive, but subject to crossreactions that make the interpretation of weakly positive results difficult. Application of Trichinella spiralis muscle larvae excretory-secretory (ES) antigens for the specific antibody detection in ELISA resulted in improved specificity compared to that of crude worm extract that was previously in use, but since production of ES has not yet been standardized, differences among laboratories occur. For this reason, the Wb profile of serum samples from different T. spiralis infected host species: human, horse, swine and dog, was investigated in the Serbian National Reference Laboratory for Trichinellosis (NRLT). The common feature of the obtained Wb profiles was the appearance of a triad of bands with molecular masses (Mw) of 45, 49, and 53 kDa. The very same triad was recognized by a monoclonal antibody (mAb) 7C2C5 specific for an immunodominant epitope unique to the muscle larvae stage of all species in the genus Trichinella. Inhibition studies confirmed that mAb and anti-Trichinella antibodies from sera competed for the same parasite epitope. Based on the obtained results, the NRLT introduced the recognition of the above mention triad as the basis for specific anti-Trichinella antibodies detection in the sera of infected hosts.

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“…It remains a serious public threat in both developed and developing countries (Murrel and Pozio, 2000;Liu and Boireau, 2002). There are eight species and three morphologically indistinguishable additional genotypes in the genus (Murrell et al 2000;Pozio et al, 2000). Trichinella spiralis (genotype-T1) is the best known species, with high infectivity to swine and rats, with cosmopolitan distribution because of passive introduction with domestic pigs and synanthropic rats.…”

Section: Introductionmentioning

confidence: 99%

Seroprevalence of Trichinella Spp. in Pigs and Knowledge, Attitude and Practices of Pig Farmers of Eastern and Midwestern Regions of Nepal

Kathayat

Shrestha

Dhakal

et al. 2015

Int J Appl Sci Biotechnol

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A cross-sectional study was conducted on four major pig raising districts of eastern and mid-western region of Nepal from February to May 2014 to find out the seroprevalence of Trichinella spp. A total of 184 serum samples of pigs were collected and antibodies against trichinella were detected using ID screen trichinella indirect multi- species ELISA kit. The study revealed prevalence rate of 3.8% and difference in prevalence rate according to age, sex, breed, rearing system, ecozone, region & district were statistically insignificant (P>0.05) as analysed by Chi-square test using PHStat version 2.5 and Fisher’s exact test. This study confirms that antibodies of Trichinella spp. are circulating in pigs of Nepal. Further, the knowledge, attitude and practices survey of meat borne helminthic zoonoses was conducted among 50 pig raisers and pork consumers by face to face interview using a semi-structure questionnaire. This survey concludes that although there were significant portion of the respondents aware of meat borne helminthic zoonoses but there were still a noticeable proportion of respondents who didn’t have a proper knowledge that upsurge public health risks. Moreover, present-day situation of their pig raising practices & pork consumption system possess them to a menace of public health zoonoses. be done for enhancing the potential of isolates. Int J Appl Sci Biotechnol, Vol 3(3): 402-407

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Detection of Trichinella infection in food animals

Cited by 76 publications

References 29 publications

Detection of Experimental Swine Trichinellosis Using Commercial Elisa Test

Detection of Experimental Swine Trichinellosis Using Commercial Elisa Test

Characteristic band pattern in western blots for specific detection of anti-Trichinella spiralis antibodies in different host species

Seroprevalence of Trichinella Spp. in Pigs and Knowledge, Attitude and Practices of Pig Farmers of Eastern and Midwestern Regions of Nepal

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