Detection of the satA gene and transferability of virginiamycin resistance in Enterococcus faecium from food-animals

Hammerum, A

doi:10.1016/s0378-1097(98)00433-9

Cited by 27 publications

(37 citation statements)

References 16 publications

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“…We also performed PCR to determine whether the streptogramin resistance genes previously found in enterococci, vatD, vatE (both encoding resistance to streptogramin A compounds), and vgbA (encoding resistance to streptogramin B compounds), and the macrolide resistance gene ermB were present in these isolates. The horizontal transfer of these genes has previously been demonstrated in vitro (12,13,18,29,30,37) and, for vatD, in vivo (16). Gene linkage between both vatD and ermB and vatE and ermB has also been demonstrated previously (5,13,18,37).…”

mentioning

confidence: 57%

Quinupristin-Dalfopristin Resistance inEnterococcus faeciumIsolates from Humans, Farm Animals, and Grocery Store Meat in the United States

et al. 2006

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Three hundred sixty-one quinupristin-dalfopristin (Q-D)-resistant Enterococcus faecium (QDREF) isolateswere isolated from humans, turkeys, chickens, swine, dairy and beef cattle from farms, chicken carcasses, and ground pork from grocery stores in the United States from 1995 to 2003. These isolates were evaluated by pulsed-field gel electrophoresis (PFGE) to determine possible commonality between QDREF isolates from human and animal sources. PCR was performed to detect the streptogramin resistance genes vatD, vatE, and vgbA and the macrolide resistance gene ermB to determine the genetic mechanism of resistance in these isolates. QDREF from humans did not have PFGE patterns similar to those from animal sources. vatE was found in 35%, 26%, and 2% of QDREF isolates from turkeys, chickens, and humans, respectively, and was not found in QDREF isolates from other sources. ermB was commonly found in QDREF isolates from all sources. Known streptogramin resistance genes were absent in the majority of isolates, suggesting the presence of other, as-yet-undetermined, mechanisms of Q-D resistance.

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confidence: 57%

Quinupristin-Dalfopristin Resistance inEnterococcus faeciumIsolates from Humans, Farm Animals, and Grocery Store Meat in the United States

et al. 2006

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“…Farms A, B, and C used 285, 345, and 6,229 g of tetracycline/year and 96, 169, and 1,136 g of sulfadiazin and trimethoprim (5:1)/year, respectively. (12). Exponentially growing donor and recipient cultures were washed twice in 0.9% saline and mixed 1:1 at an optical density at 450 nm of 0.5.…”

Section: Farmsmentioning

confidence: 99%

Class 1 Integrons and Tetracycline Resistance Genes in Alcaligenes , Arthrobacter , and Pseudomonas spp. Isolated from Pigsties and Manured Soil

Agersø

Sandvang

2005

Appl Environ Microbiol

134

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The presence of tetracycline resistance (Tc r ) genes and class I integrons (in-1), and their ability to cotransfer were investigated in Tc r gram-negative (185 strains) and gram-positive (72 strains) bacteria from Danish farmland and pigsties. The isolates belonged to the groups or species Escherichia coli, Enterobacter spp., Arthrobacter spp., Alcaligenes spp., Pseudomonas spp., and Corynebacterium glutamicum. The 257 isolates were screened for in-1. Eighty-one of the gram-negative isolates were also screened for the Tc r genes tet(A), tet(B), and tet(C), and all (n ‫؍‬ 72) gram-positive isolates were screened for tet(33). Fourteen (7%) of the soil isolates and eleven (25%) of the pigsty isolates contained in-1. All isolates that contained tet genes also contained in-1, except one gram-negative isolate from a pigsty that contained tet(B). All gram-positive isolates with in-1 also contained tet(33). No isolates contained more than one tet gene. The in-1-positive isolates were tested for resistance to selected antimicrobial agents and showed resistance to three to nine drugs. Filter-mating experiments showed cotransfer of Tc r and class I integrons from soil isolates to Escherichia coli and/or Pseudomonas putida. We conclude that soil bacteria in close contact to manure or pigsty environment may thus have an important role in horizontal spread of resistance. Use of tetracyclines in food animal production may increase not only Tc r but also multidrug resistance (caused by the presence tet genes and in-1) in bacteria.

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“…These ®ndings presumably prompted investigations into resistance to other antibiotics of interest (or potential interest) in human medicine. Other types of antibiotic resistance reported in enterococcal isolates from animals include resistance to the macrolide± lincosamide ±streptogramin group (Dutta & Devriese, 1982), to tylosin (Aarestrup & Cartensen, 1998) and to virginiamycin (Hammerum et al 1998). An Australian study (MD Barton and R Pratt, unpublished results) found widespread resistance to: macrolides (erythromycin and tylosin); clindamycin, tiamulin and virginiamycin; aminoglycosides (streptomycin, gentamicin, apramycin); spectinomycin, tetracycline and monensin.…”

Section: Antibiotic Resistance In Enteric Bacteriamentioning

confidence: 99%

Antibiotic use in animal feed and its impact on human healt

2000

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Antibiotic resistance in bacteria that cause disease in man is an issue of major concern. Although misuse of antibiotics in human medicine is the principal cause of the problem, antibiotic-resistant bacteria originating in animals are contributory factors, with some types of resistance in some species of bacteria. Antibiotics are added to animal feeds to treat and prevent infections and to improve growth and production. Until recently, the major concerns about incorporation of antibiotics in animal feeds related to antibiotic residues in products from treated animals. Although, in 1969, the Swann (1969) report drew attention to the potential for antibiotic-resistant bacteria to spread from treated animals via the food chain, there was little response until the detection of vancomycin-resistant enterococci in animals fed a related glycopeptide, avoparcin. Subsequently, attention started to focus on the issue and other examples of transfer of resistant bacteria through the food chain, such as enterococci resistant to quinupristin–dalfopristin or to everninomicin, fluoroquinolone-resistant campylobacters and multiresistantEscherichia coli, and salmonella such asSalmonella typhimuriumDT104. Reviews and committees in many countries have highlighted the need for better control of licensing of antibiotics, and codes for prudent use of antibiotics by veterinary practitioners and farmers. The continued use of antibiotic growth promoters has been questioned and there is a need to ensure that antibiotics important in human medicine are not used therapeutically or prophylactically in animals.

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Detection of the satA gene and transferability of virginiamycin resistance in Enterococcus faecium from food-animals

Cited by 27 publications

References 16 publications

Quinupristin-Dalfopristin Resistance inEnterococcus faeciumIsolates from Humans, Farm Animals, and Grocery Store Meat in the United States

Quinupristin-Dalfopristin Resistance inEnterococcus faeciumIsolates from Humans, Farm Animals, and Grocery Store Meat in the United States

Class 1 Integrons and Tetracycline Resistance Genes in Alcaligenes , Arthrobacter , and Pseudomonas spp. Isolated from Pigsties and Manured Soil

Antibiotic use in animal feed and its impact on human healt

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