Detection of the putative cis- region involved in the induction by a Pyricularia oryzae elicitor of the promoter of a gene encoding phenylalanine ammonia-lyase in rice

Abstract

A rice PAL (phenylalanine ammonia-lyase) gene sequence ( rPAL-P5), which is highly similar to and likely the same as a previously described rice ZB8PAL gene, including the 5'-upstream and exon I coding regions of PAL, was isolated using PCR amplification. The expression of several PALs, including rPAL-P5, was strongly induced following inoculation with Pyricularia oryzae or treatment with a P. oryzae elicitor. To identify the promoter region induced by the P. oryzae elicitor, we constructed and subsequently transformed rPAL-P5 promoter deletion series into rice calli using particle bombardment. Results from both elicitor-inducible reporter gene and gel mobility shift assays demonstrated that the sequence -349 to -256 of the rPAL-P5 promoter includes a cis-element involved in the induction of P. oryzae.

Elicitor and Calatalse Activity of Conidia Suspensions of Various Strains ofMagnaporthe griseain Suspension-Cultured Cells of Rice

“…8) Hydrolytic products of cell-wall components such as chitin and -glucans are also potent elicitors. 1,9,10) The gene-for-gene relationship in suspension-cultured cells has been reported for tobacco 11) and soybean, 12) but the fungal avirulence gene by suspension-cultured cells has been recognized only for tobacco cells inoculated with conidia of an oomycetes, Phytophthora infestance.…”

Enhancement of the expression of defense genes in tomato against Ralstonia solanacearum by N-octanoyl-L-homoserine lactone

“…Early induction of PAL is more important because it is the first key regulatory enzyme in the phenyl propanoid pathway leading to the production of phytoalexins and phenolic substances (Wang et al, 2004). In this study, maximum PAL activity was 9 h after inoculation (hpi).…”

Defense responses in tomato landrace and wild genotypes to early blight pathogenAlternaria solaniinfection and accumulation of pathogenesis-related proteins

“…Since phytoalexins and phenolic substances, products of phenylpropanoid pathway, are regulatory and restrictive substances in pathogen infection, induction of PAL is pivotal being the first committed key regulatory enzyme in that pathway (Wang et al 2004). Our study revealed that PAL activity was maximum at 2-d after inoculation, when compared with its control and the other samples of different time intervals assayed.…”