Elicitor and Calatalse Activity of Conidia Suspensions of Various Strains of<i>Magnaporthe grisea</i>in Suspension-Cultured Cells of Rice

Tanabe, Shigeru; Hayashi, Nagao; Yamane, Hisakazu; Shibuya, Naoto; Minami, Eiichi

doi:10.1271/bbb.70684

Cited by 13 publications

(8 citation statements)

References 13 publications

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“…On the other hand, pr1 is indicative of biotic stress [32,33]. The gene expression pattern of cat1 , pal , and pr1 , by effect of elicitors’ application at different times in C. annuum L. var.…”

Section: Resultsmentioning

confidence: 99%

Oxidative and Molecular Responses in Capsicum annuum L. after Hydrogen Peroxide, Salicylic Acid and Chitosan Foliar Applications

Mejía-Teniente

Durán-Flores

Chapa-Oliver

et al. 2013

IJMS

100

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Hydrogen peroxide (H2O2) is an important ROS molecule (Reactive oxygen species) that serves as a signal of oxidative stress and activation of signaling cascades as a result of the early response of the plant to biotic stress. This response can also be generated with the application of elicitors, stable molecules that induce the activation of transduction cascades and hormonal pathways, which trigger induced resistance to environmental stress. In this work, we evaluated the endogenous H2O2 production caused by salicylic acid (SA), chitosan (QN), and H2O2 elicitors in Capsicum annuum L. Hydrogen peroxide production after elicitation, catalase (CAT) and phenylalanine ammonia lyase (PAL) activities, as well as gene expression analysis of cat1, pal, and pathogenesis-related protein 1 (pr1) were determined. Our results displayed that 6.7 and 10 mM SA concentrations, and, 14 and 18 mM H2O2 concentrations, induced an endogenous H2O2 and gene expression. QN treatments induced the same responses in lesser proportion than the other two elicitors. Endogenous H2O2 production monitored during several days, showed results that could be an indicator for determining application opportunity uses in agriculture for maintaining plant alert systems against a stress.

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Section: Resultsmentioning

confidence: 99%

Oxidative and Molecular Responses in Capsicum annuum L. after Hydrogen Peroxide, Salicylic Acid and Chitosan Foliar Applications

Mejía-Teniente

Durán-Flores

Chapa-Oliver

et al. 2013

IJMS

100

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“…Since in KatGs K m significantly increases below pH optimum with decreasing pH [58] MagKatG2 is the first KatG that has a K m value in the low millimolar region around pH 5. Recent data suggest that extracellular KatG acts primarily as catalase [9] and the observed acidic pH optimum reflects the milieu in which the extracellular fungal enzyme armory has to degrade the reactive oxygen species derived from the oxidative burst induced by the plant tissue [59]. Moreover, in the same pH region defense-related genes of the plant have been shown to be expressed [59].…”

Section: Discussionmentioning

confidence: 99%

Eukaryotic extracellular catalase–peroxidase from Magnaporthe grisea – Biophysical/chemical characterization of the first representative from a novel phytopathogenic KatG group

et al. 2012

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All phytopathogenic fungi have two catalase–peroxidase paralogues located either intracellularly (KatG1) or extracellularly (KatG2). Here, for the first time a secreted bifunctional, homodimeric catalase–peroxidase (KatG2 from the rice blast fungus Magnaporthe grisea) has been produced heterologously with almost 100% heme occupancy and comprehensively investigated by using a broad set of methods including UV–Vis, ECD and resonance Raman spectroscopy (RR), thin-layer spectroelectrochemistry, mass spectrometry, steady-state & presteady-state spectroscopy. RR spectroscopy reveals that MagKatG2 shows a unique mixed-spin state, non-planar heme b, and a proximal histidine with pronounced imidazolate character. At pH 7.0 and 25 °C, the standard reduction potential E°′ of the Fe(III)/Fe(II) couple for the high-spin native protein was found to fall in the range typical for the KatG family. Binding of cyanide was relatively slow at pH 7.0 and 25 °C and with a Kd value significantly higher than for the intracellular counterpart. Demonstrated by mass spectrometry MagKatG2 has the typical Trp118-Tyr251-Met277 adduct that is essential for its predominantly catalase activity at the unique acidic pH optimum. In addition, MagKatG2 acts as a versatile peroxidase using both one- and two-electron donors. Based on these data, structure–function relationships of extracellular eukaryotic KatGs are discussed with respect to intracellular KatGs and possible role(s) in host–pathogen interaction.

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“…POD participates in a variety of plant defence mechanisms, and is involved in plant resistance against certain diseases (Silva et al, 2008;Dutsadee and Nunta, 2008). SOD, POD and CAT, which play important roles in the metabolism of reactive oxygen species, could be induced by environmental stresses, including fungal elicitors (Pachten and Barz, 1999;Tanabe et al, 2008). Our results showed that POD activity was up-regulated to eliminate the H 2 O 2 produced by SOD in chronological order (Figure 1).…”

Section: Discussionmentioning

confidence: 70%

Time and dosage effects of an endophytic fungal elicitor on the volatile oil production and physiology of Atractylodes lancea suspension cells

Ren¹,

Yan²,

Fang³

et al. 2012

J. Med. Plants Res.

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The effects of an endophytic fungal elicitor from Cunninghamella sp. (AL4) on the volatile oil production and defence responses of host plant Atractylodes lancea were investigated. The aim was to improve the capability of endophytic fungi resources in promoting medicinal plant secondary metabolites. The results showed that the endophytic fungal elicitor significantly stimulated volatile oil accumulation. The best inducing concentration of the elicitor for volatile oil production was 30 mgL -1 . The highest amount of volatile oil was harvested 9 days (d) after elicitor treatment. The activity of 3-hydroxy-3-methylglutaryl CoA reductase increased by 2.79-folds compared with the control of 7 d after treatment. The elicitor also enhanced the activities of phenylalanine ammonia-lyase, peroxidase superoxide dismutase, polyphenol oxidase, and catalase. These enhanced activities led to improved plant defence-related secondary metabolite synthesis ability. In addition, the elicitor-treated cells were consistently healthy, and had slightly increased biomass. The results demonstrated for the first time the optimal induction time and concentration of the endophytic fungal elicitor, as well as the changes in physiological indices after endophyte-host interaction. The information provided in the present study may be applied in large-scale medicinal plant suspension cultures.

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Elicitor and Calatalse Activity of Conidia Suspensions of Various Strains ofMagnaporthe griseain Suspension-Cultured Cells of Rice

Cited by 13 publications

References 13 publications

Oxidative and Molecular Responses in Capsicum annuum L. after Hydrogen Peroxide, Salicylic Acid and Chitosan Foliar Applications

Oxidative and Molecular Responses in Capsicum annuum L. after Hydrogen Peroxide, Salicylic Acid and Chitosan Foliar Applications

Eukaryotic extracellular catalase–peroxidase from Magnaporthe grisea – Biophysical/chemical characterization of the first representative from a novel phytopathogenic KatG group

Time and dosage effects of an endophytic fungal elicitor on the volatile oil production and physiology of Atractylodes lancea suspension cells

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