Detection of the First QnrS Gene Positivity in Aquatic Aeromonas spp. Isolates in Turkey

Onuk, Ertan Emek; Çaycı, Yeliz Tanrıverdi; Çoban, Ahmet Yılmaz; Çiftci, Alper; Didinen, Behire Işıl; Altun, Soner; ÜNLÜ, Mehtap SÖĞÜT; Deveci, Aydın

doi:10.5578/mb.8839

Cited by 7 publications

(3 citation statements)

References 22 publications

(32 reference statements)

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“…Today, while most mesophilic bacteria are registered in the databases of these identification systems and are regularly updated, there is still no comprehensive database available for fish pathogens (www.biomerieux-diagnostics.com; www.bd.com). Among the identification test kits most commonly used for aquatic pathogens, the API 20E test system, although initially having been designed for enteric pathogens, can be also used for the identification of non-enteric Aeromonas species (Onuk et al 2015;Stratev and Odeyemi 2016;El-Son et al 2019). There are many reports of low success rates for rapid diagnostic systems in determining aquatic pathogens, thus indicating the unfeasibility of these systems for such use (Santos et al 1993;Jin et al 2011;Janda 2014;Popović et al 2017;Jung-Schroers et al 2018).…”

Section: Colorimetric Methods By Automated Instrumentsmentioning

confidence: 99%

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General Assessment of Approaches to the Identification of Aquatic Bacterial Pathogens: A Methodological Review

2022

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In the evolving biosphere, pathogenic microorganisms that cause disease may undergo phenotypic changes. While some of these changes result in new variants or mutants, others lead to the emergence of novel pathogens. Such phenotypic changes as well as advances in technology and analytical methods and the identification of genomic sequences of microbial DNA have brought about new methodological approaches in the diagnosis of bacterial diseases. Although bacterial identification was originally based on phenotypic characteristics, later researchers claimed that bacteria could be accurately identified by only gene sequencing and generally by the sequencing of the 16S ribosomal RNA gene region. Currently, there is still disagreement between classical microbiologists and those using new genomic sequence technology over the best method for identification. Fish are cold‐blooded animals, and fish pathogens generally exhibit psychrophilic characteristics. Many bacterial identification systems that are used to identify mesophilic bacteria remain useless for identifying fish pathogens because the optimum incubation temperatures for mesophilic bacteria are 35–37°C. Bacteria that are pathogenic to piscine species require specific media for their cultivation at lower incubation temperatures (15–28°C), and this limits both their growth in culture and subsequent identification by phenotype‐based methods. This review presents a comprehensive overview of the isolation and identification of bacterial fish pathogens by optimal culture conditions, biochemical tests, colorimetric methods for rapid identification systems, matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry, and immunological and molecular methods, as well as an overview of the detection of uncultivable bacteria and the use of anamnesis. We conclude that the accurate identification of fish pathogens requires the use of different methods, including phenotype‐ and genotype‐based tests, and the evaluation of anamnesis.

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Section: Colorimetric Methods By Automated Instrumentsmentioning

confidence: 99%

“…Among the identification test kits most commonly used for aquatic pathogens, the API 20E test system, although initially having been designed for enteric pathogens, can be also used for the identification of non‐enteric Aeromonas species (Onuk et al. 2015; Stratev and Odeyemi 2016; El‐Son et al. 2019).…”

Section: Phenotypic Approachmentioning

confidence: 99%

General Assessment of Approaches to the Identification of Aquatic Bacterial Pathogens: A Methodological Review

2022

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“…Some scientific studies indicate that several diseases were affecting wild fish populations before the aquaculture industry existed (Olivier, 2012). The studies related to fish diseases such as pseudomoniasis,pasteurellosis, motile Aeromonas septicemia and lactococcosis generally focused on the reared fish species in Turkey (Korun & Timur, 2005;Onuk et al 2015;Ture & Alp, 2016).…”

Section: Introductionmentioning

confidence: 99%

Untitled

Türe¹,

Misir²,

Altuntas³

et al. 2018

Turk. J. Fish. Aquat. Sci.

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In the present study, a total of 180 whiting(Merlangius merlanguseuxinus) were caught in the Eastern Black Sea coast of Turkey and investigated through for six month period for their bacteria. The bacterial agents isolated from fish were identified by Analytical Profile Index (API 20NE, 20E and rapid ID 32 Strep). Fifty-two bacteria were further confirmed by 16S rRNA gene sequencing. Nine bacterial species, Pseudomonas luteola, Staphylococcus equorum, Vibrio anguillarum, Pseudomonas putida, Acinetobacter johnsonii, Pseudomonas protogens, Oceanisphaera profunda, Pseudomonas fluorescens,and Serratia fonticolawere identified. This study is the first report on the bacteria of whitingin Turkey and provides significant information in terms of bacterial fish pathogens of this area.

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Plasmid-mediated quinolone resistance in Enterobacteriaceae: a systematic review with a focus on Mediterranean countries

Yanat

Rodríguez-Martínez

Touati

2016

Eur J Clin Microbiol Infect Dis

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Quinolones are a family of synthetic broad-spectrum antimicrobial drugs. These molecules have been widely prescribed to treat various infectious diseases and have been classified into several generations based on their spectrum of activity. Quinolones inhibit bacterial DNA synthesis by interfering with the action of DNA gyrase and topoisomerase IV. Mutations in the genes encoding these targets are the most common mechanisms of high-level fluoroquinolone resistance. Moreover, three mechanisms for plasmid-mediated quinolone resistance (PMQR) have been discovered since 1998 and include Qnr proteins, the aminoglycoside acetyltransferase AAC(6')-Ib-cr, and plasmid-mediated efflux pumps QepA and OqxAB. Plasmids with these mechanisms often encode additional antimicrobial resistance (extended spectrum beta-lactamases [ESBLs] and plasmidic AmpC [pAmpC] ß-lactamases) and can transfer multidrug resistance. The PMQR determinants are disseminated in Mediterranean countries with prevalence relatively high depending on the sources and the regions, highlighting the necessity of long-term surveillance for the future monitoring of trends in the occurrence of PMQR genes.

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Detection of the First QnrS Gene Positivity in Aquatic Aeromonas spp. Isolates in Turkey

Cited by 7 publications

References 22 publications

General Assessment of Approaches to the Identification of Aquatic Bacterial Pathogens: A Methodological Review

General Assessment of Approaches to the Identification of Aquatic Bacterial Pathogens: A Methodological Review

Untitled

Plasmid-mediated quinolone resistance in Enterobacteriaceae: a systematic review with a focus on Mediterranean countries

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