Detection of the contamination sources of Listeria monocytogenes in pickled white cheese production process line and genotyping with the pulsed-field gel electrophoresis method

Telli, Nihat; Güner, Ahmet; Dönmez, Ferda Soyer; Özdemir, Özgün Öykü

doi:10.3906/vet-1511-59

Cited by 2 publications

(3 citation statements)

References 23 publications

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“…L. monocytogenes was not isolated from white cheese. In other reports conducted with white cheese sold in Turkey, isolation rates of L. monocytogenes was 6% in Afyonkarahisar (Akkaya and Alişarlı, 2006), 9.5% in Kayseri (Kum et al, 2011), 3% in Erzincan (Azak et al, 2012), 2% in Balıkesir (Gökmen et al, 2016), 15.77% in Tekirdağ (Kaptan, 2016, and 3.53% in Konya (Telli et al, 2016). These results mentioned above were higher than our findings.…”

Section: Resultsmentioning

confidence: 89%

“…Contamination of raw milk may occur in a dairy farm. In addition, the source of L. monocytogenes contaminations could be: i) poor quality of silage, ii) inadequate cleanliness of animals, iii) poor hygienic conditions which could occur during milking, storage and transport, iv) infected cows in dairy farms (Marnissi et al, 2013;Telli et al, 2016).…”

Section: Resultsmentioning

confidence: 99%

“…However, cross contamination is a major problem during the cheese production. Contamination sources may be: i) the contaminated raw milk, ii) environment conditions and unclean equipments, and iii) insufficient heat treatment of milk to kill the organisms (Kasalica et al, 2011;Telli et al, 2016). In addition, there are several factors of reproducing of Listeria spp.…”

Section: Resultsmentioning

confidence: 99%

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Detection of Listeria Spp. In Raw Milk and Dairy Products Retailed in Ankara

Şanlibaba

Tezel

Çakmak³

2018

Gıda

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The objective of this study was to determine the prevalence of Listeria species in raw milk, and dairy products. A total of 110 samples were obtained from randomly selected retail stores and local bazaars located in Ankara. Using TS EN ISO 11290-1 method, 23 samples were found positive for Listeria spp. The overall prevalence of Listeria spp. was 20.91%, in which L. innocua was the most commonly recovered species (6.36%). The remaining isolates were identified as L ivanovii (5.45%), L. monocytogenes (4.55%), and L welshimeri (4.55%). The L monocytogenes isolates were positive for the presence of hlyA gene. The highest prevalence of Listeria spp. was found in homemade cheese (9.09%), followed by raw milk (8.19%), and white cheese (3.64%). L monocytogenes was isolated from raw milk and homemade cheese in this study. In conclusion, the low hygienic quality dairy products may lead to listeriosis surveillance in Ankara.

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Section: Resultsmentioning

confidence: 89%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Detection of Listeria Spp. In Raw Milk and Dairy Products Retailed in Ankara

Şanlibaba

Tezel

Çakmak³

2018

Gıda

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Prevalence and antimicrobial resistance of Listeria species and molecular characterization of Listeria monocytogenes isolated from retail ready-to-eat foods

Arslan

Özdemir

2020

FEMS Microbiology Letters

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A wide variety of foods can be contaminated with Listeria species, especially L. monocytogenes. Ready-to-eat (RTE) foods are predominantly associated with human listeriosis caused by L. monocytogenes. Therefore, this study aimed to assess the presence of Listeria species in RTE foods and to characterize L. monocytogenes isolates by means of detection of virulence markers, serotypes and genetic relatedness. Of the 300 RTE food samples, 59 (19.7%) were positive for Listeria species: L. innocua (13.3%), L. monocytogenes (5%), L. welshimerii (2.3%), L. grayi subsp. murrayi (1.3%), L. grayi (1%), L. ivanovii (1%) and L. ivanovi subsp. londoniensis (0.3%). All L. monocytogenes isolates identified were positive for the actA, iap, inlA, inlB, inlC, inlJ, plcA and prfA virulence genes and biofilm. The isolates were serotyped as 1/2c (33.3%), 4b (26.7%), 1/2a (26.7%), 1/2b (6.7%) and 3c (6.7%) by the multiplex-PCR and agglutination methods. PCR-restriction fragment length polymorphism with AluI and MluCI resulted in three and two profiles, respectively. Pulsed-field gel electrophoresis differentiated the L. monocytogenes isolates into 15 ApaI and 12 AscI patterns. Antimicrobial resistance of all Listeria isolates was determined by the disk diffusion method. Most L. monocytogenes isolates were sensitive to antimicrobials used in the treatment of listeriosis. This study shows the presence of potential pathogenic and antimicrobial-resistant L. monocytogenes in RTE foods that may lead to consumer health risks.

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Detection of the contamination sources of Listeria monocytogenes in pickled white cheese production process line and genotyping with the pulsed-field gel electrophoresis method

Cited by 2 publications

References 23 publications

Detection of Listeria Spp. In Raw Milk and Dairy Products Retailed in Ankara

Detection of Listeria Spp. In Raw Milk and Dairy Products Retailed in Ankara

Prevalence and antimicrobial resistance of Listeria species and molecular characterization of Listeria monocytogenes isolated from retail ready-to-eat foods

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