Detection of Listeria Spp. In Raw Milk and  Dairy Products Retailed in Ankara

Şanlibaba, Pınar; Tezel, Başar Uymaz; Çakmak, Gürcü Aybige

doi:10.15237/gida.gd17107

Cited by 14 publications

(14 citation statements)

References 39 publications

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“…Genes iap and hlyA are responsible for host cell invasion, while the actA gene is associated with cell-to-cell spread. Our finding agreed with those of previous studies conducted by Tahoun et al, and Şanlibaba et al [ 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 ], in which virulence genes were determined in L. monocytogenes isolated from raw milk, animals, and environmental samples. Our findings in virulence gene detection are also supported by the findings of [ 23 ], who reported that virulence genes hlyA , iap, and actA were the most recognized, with prevalence rates of 70.6%, 70.6%, and 52.9%, respectively, and two of the three virulence genes ( hlyA , iap or actA ) were simultaneously detected in six isolates.…”

Section: Discussionsupporting

confidence: 94%

Prevalence and Antimicrobial Resistance of Virulent Listeria monocytogenes and Cronobacter sakazakii in Dairy Cattle, the Environment, and Dried Milk with the In Vitro Application of Natural Alternative Control

et al. 2022

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This study aims to detect the prevalence and antimicrobial resistance of Listeria monocytogenes and Cronobacter sakazakii in three dairy households and dried milk from different suppliers, and evaluate the antimicrobial effect of rose water, rose, and orange essential oils. In total, 360 samples were collected from cattle, the environment, and dried milk (n = 30). Antimicrobial activity was evaluated with twofold microtube dilution and the time-kill method. L. monocytogenes was identified in all households (13.3%) with a prevalence in the range of 5.8–17.5%, while C. sakazakii was identified in one household (5.3%). The former and latter pathogens were highly isolated from the feces at 20% and 2.5% and bedding at 12.5% and 1.6%, respectively. L. monocytogenes was isolated only from milk at 7.5%, but C. sakazakii was not detected in either milk or dried milk. L. monocytogenes strains were screened for virulence genes (iap, hylA, and actA). All strains were positive for the iap gene, while for hlyA and actA, the percentages were (35.4% 16.6%, respectively). L. monocytogenes strains showed high resistance against sulfamethoxazole–trimethoprim (100%), followed by gentamicin, penicillin, and imipenem (95.8%, 95.8%, and 91.6%, respectively). All C. sakazakii strains were susceptible to all tested antibiotics. The bactericidal activity of orange oil was the strongest, appeared after 1 h for both pathogens, followed by rose oil and then rose water.

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Section: Discussionsupporting

confidence: 94%

Prevalence and Antimicrobial Resistance of Virulent Listeria monocytogenes and Cronobacter sakazakii in Dairy Cattle, the Environment, and Dried Milk with the In Vitro Application of Natural Alternative Control

et al. 2022

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“…The prevalence of L. ivanovii and L. welshimeri from milk of dairy cows were 6% and 4%, respectively in this study. In Turkey, nearly similar finding for L. ivanovii (5.4%) and L. welshimeri (4.5%) isolates from milk and milk products were detected by Sanlibaba et al [35]. On the other hand, lower prevalence of L. ivanovii (1.4%) and L. welshimeri (0.7%) in previous study were detected from cow milk samples by El-Sherbini et al [36] in Sharkia Governorate.…”

Section: Discussionsupporting

confidence: 73%

Prevalence of Listeria Species in Dairy Cows and Pregnant Women with Reference to Virulotyping of Listeria monocytogenes in Egypt

El-Naenaeey

Abdelwahab

Merwad

et al. 2019

Zagazig Veterinary Journal

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This study was conducted to determine the prevalence of Listeria spp. in milk and feces of dairy cows as well as stool of pregnant women in Sharkia and Dakahlia Governorates, Egypt. Three hundred and fifty samples including; normal raw milk (n=200), mastitis milk (n=50), feces of dairy cattle (n=50) and stool of pregnant women (n=50) were randomly collected. Listeria spp. were isolated on Oxford agar and then subjected to biochemical and molecular identification. The overall isolation rate of Listeria spp. was 16%. The prevalence rates of Listeria monocytogenes were 8%, 4%, 2% and 4% in feces of dairy cows, normal milk, mastitis milk and stool of pregnant women, respectively. The prevalence of L. ivanovii and L. welshimeri in milk of dairy cows were 6% and 4%, respectively. L. monocytogenes isolates were molecularly confirmed to the species level by amplification of 16S rRNA gene. The distribution of internalin A (inlA) and internalin B (inlB) was determined in 15 identified L. monocytogenes isolates. The overall prevalence of inlA gene and inlB gene in L. monocytogenes isolates from different sources were 80% and 40%, respectively. Two L. monocytogenes isolates from normal raw milk and feces of dairy cows didn't harbor both inlA and inlB genes. Listeria spp. isolated from normal milk, feces, mastitis milk and stool of pregnant women, potentially can cause human illness and abortion in pregnant women. Internalin (A&B) genes are considered the best indicator for virulence determination of L. monocytogenes isolated from different sources.

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“…Aksoy et al [16] found L. monocytogenes in 16 (5.3%) dairy products in 300 food samples . Şanlıbaba et al [17] raported that Listeria spp. the prevalence were found in homemade cheese (9.09%), raw milk (8.19%) and white cheese (3.64%), respectively.…”

Section: Discussionmentioning

confidence: 99%

Taze Kaşar Peyniri ve Süt Kaymağından İzole Edilen Listeria monocytogenes ve Esherichia coli O157:H7’nin Moleküler Tanımlaması

Ekici¹,

Dümen²,

Bayrakal³

et al. 2018

Kafkas Univ Vet Fak Derg

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In this study, it is aimed to analyze the risk factors related to Listeria monocytogenes and Esherichia coli O157:H7 monitoring in fresh kashar cheese and milk creme in the province of Istanbul. It has 100 pieces of fresh kashar cheese and 100 pieces of milk creme material. Samples were confirmed by PCR methods and genetic basis with conventional microbiological sowing methods and analysis methods. Another case for the study is to obtain significant epidemiological environment for the field strains in the country in terms of L. monocytogenes and E. coli OH157: H7, where high polymorphic protein structures and rapid genetic variation exist. In the study, 4 (2%) of E. coli OH157: H7 and 6 (3%) of L. monocytogenes were found among 200 milk slices and fresh kashar cheese samples. Those microorganisms can easily reproduce in natural environment such as soil and silage and with primer/secondary contamination, they appear to be health threatening food related pathogens. It is suggested to have studies for optimal food safety and this can be provided in accordance with appropriate legislation.

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Detection of Listeria Spp. In Raw Milk and Dairy Products Retailed in Ankara

Cited by 14 publications

References 39 publications

Prevalence and Antimicrobial Resistance of Virulent Listeria monocytogenes and Cronobacter sakazakii in Dairy Cattle, the Environment, and Dried Milk with the In Vitro Application of Natural Alternative Control

Prevalence and Antimicrobial Resistance of Virulent Listeria monocytogenes and Cronobacter sakazakii in Dairy Cattle, the Environment, and Dried Milk with the In Vitro Application of Natural Alternative Control

Prevalence of Listeria Species in Dairy Cows and Pregnant Women with Reference to Virulotyping of Listeria monocytogenes in Egypt

Taze Kaşar Peyniri ve Süt Kaymağından İzole Edilen Listeria monocytogenes ve Esherichia coli O157:H7’nin Moleküler Tanımlaması

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