Detection of Sulfonamide Resistance Genes via in situ PCR-FISH

Gnida, Anna; Kunda, Katarzyna; Ziembińska, A.; Łuczkiewicz, Aneta; Felis, Ewa; Surmacz-Górska, Joanna

doi:10.33073/pjm-2014-022

Cited by 4 publications

(2 citation statements)

References 28 publications

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“…The activated sludge has been already reported as a niche rich in sul 1–3 and integrons (e.g., Tennstedt et al 2003 ). Those resistance determinants were also detected in the activated sludge samples obtained from the same WWTP and used in the previous study (Luczkiewicz et al 2013 ; Gnida et al 2014 ). In wastewater processes, class 1 integrons are suggested to serve as an important reservoir of resistance genes, mainly those encoding resistance to disinfectants ( qac EΔ1) and sulfonamides ( sul 1) (Enne et al 2001 ; Huovinen et al 1995 ; Huovinen 2001 ; Sköld 2001 ).…”

Section: Resultssupporting

confidence: 54%

The treatment of wastewater containing pharmaceuticals in microcosm constructed wetlands: the occurrence of integrons (int1–2) and associated resistance genes (sul1–3, qacEΔ1)

Nowrotek

Kotlarska

Łuczkiewicz

et al. 2017

Environ Sci Pollut Res

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The aim of this study was to analyze the occurrence of sulfonamide resistance genes (sul1–3) and other genetic elements as antiseptic resistance gene (qacEΔ1) and class 1 and class 2 integrons (int1–2) in the upper layer of substrate and in the effluent of microcosm constructed wetlands (CWs) treating artificial wastewater containing diclofenac and sulfamethoxazole (SMX), which is a sulfonamide antibiotic. The bacteria in the substrate and in the effluents were equipped with the sul1–2, int1, and qacEΔ1 resistance determinants, which were introduced into the CW system during inoculation with activated sludge and with the soil attached to the rhizosphere of potted seedlings of Phalaris arundinacea ‘Picta’ roots (int1). By comparing the occurrence of the resistance determinants in the upper substrate layer and the effluent, it can be stated that they neither were lost nor emerged along the flow path. The implications of the presence of antibiotic resistance genes in the effluent may entail a risk of antibiotic resistance being spread in the receiving environment. Additionally, transformation products of SMX were determined. According to the obtained results, four (potential) SMX transformation products were identified. Two major metabolites of SMX, 2,3,5-trihydroxy-SMX and 3,5-dihydroxy-SMX, indicated that SMX may be partly oxidized during the treatment. The remaining two SMX transformation products (hydroxy-glutathionyl-SMX and glutathionyl-SMX) are conjugates with glutathione, which suggests the ability of CW bacterial community to degrade SMX and resist antimicrobial stress.Electronic supplementary materialThe online version of this article (doi:10.1007/s11356-017-9079-1) contains supplementary material, which is available to authorized users.

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Section: Resultssupporting

confidence: 54%

The treatment of wastewater containing pharmaceuticals in microcosm constructed wetlands: the occurrence of integrons (int1–2) and associated resistance genes (sul1–3, qacEΔ1)

Nowrotek

Kotlarska

Łuczkiewicz

et al. 2017

Environ Sci Pollut Res

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“…Numerous studies have shown that ARB and ARGs related to quinolones (Lindberg et al, 2006; Kaplan et al, 2013), tetracyclines (Huang et al, 2014; Li et al, 2013), and sulfonamides (Collado et al, 2013; Gnida et al, 2014) could be found within various types of biosolids (nutrient rich organic matter that is a by‐product of wastewater and sewage sludge treatment). Additionally, several antibiotic resistance plasmids including MDR pAS1 and RP4 carrying resistance to tetracycline, chloramphenicol, ampicillin, kanamycin, and many other antibiotics have been found in the diverse bacterial communities of activated sludge (Dröge et al, 2000; Parsley et al, 2010; Soda et al, 2008; Zhang et al, 2011).…”

Section: Challenges In Measuring Background Levelsmentioning

confidence: 99%

How Should We Be Determining Background and Baseline Antibiotic Resistance Levels in Agroecosystem Research?

et al. 2016

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Although historically, antibiotic resistance has occurred naturally in environmental bacteria, many questions remain regarding the specifics of how humans and animals contribute to the development and spread of antibiotic resistance in agroecosystems. Additional research is necessary to completely understand the potential risks to human, animal, and ecological health in systems altered by antibiotic-resistance-related contamination. At present, analyzing and interpreting the effects of human and animal inputs on antibiotic resistance in agroecosystems is difficult, since standard research terminology and protocols do not exist for studying background and baseline levels of resistance in the environment. To improve the state of science in antibiotic-resistance-related research in agroecosystems, researchers are encouraged to incorporate baseline data within the study system and background data from outside the study system to normalize the study data and determine the potential impact of antibiotic-resistance-related determinants on a specific agroecosystem. Therefore, the aims of this review were to (i) present standard definitions for commonly used terms in environmental antibiotic resistance research and (ii) illustrate the need for research standards (normalization) within and between studies of antibiotic resistance in agroecosystems. To foster synergy among antibiotic resistance researchers, a new surveillance and decision-making tool is proposed to assist researchers in determining the most relevant and important antibiotic-resistance-related targets to focus on in their given agroecosystems. Incorporation of these components within antibiotic-resistance-related studies should allow for a more comprehensive and accurate picture of the current and future states of antibiotic resistance in the environment.

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Impact of flooding on urban soils: Changes in antibiotic resistance and bacterial community after Hurricane Harvey

Pérez-Valdespino

Pircher

Pérez-Domínguez

et al. 2021

Science of The Total Environment

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Detection of Sulfonamide Resistance Genes via in situ PCR-FISH

Cited by 4 publications

References 28 publications

The treatment of wastewater containing pharmaceuticals in microcosm constructed wetlands: the occurrence of integrons (int1–2) and associated resistance genes (sul1–3, qacEΔ1)

The treatment of wastewater containing pharmaceuticals in microcosm constructed wetlands: the occurrence of integrons (int1–2) and associated resistance genes (sul1–3, qacEΔ1)

How Should We Be Determining Background and Baseline Antibiotic Resistance Levels in Agroecosystem Research?

Impact of flooding on urban soils: Changes in antibiotic resistance and bacterial community after Hurricane Harvey

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