Impact of flooding on urban soils: Changes in antibiotic resistance and bacterial community after Hurricane Harvey

Pérez-Valdespino, Abigail; Pircher, Ryan; Pérez-Domínguez, Citlali Y.; Mendoza‐Sanchez, Itza

doi:10.1016/j.scitotenv.2020.142643

Cited by 15 publications

(6 citation statements)

References 55 publications

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“…Nevertheless, prediction of ARG and virulence factors with PICRUSt2 in samples collected immediately following HH suggests that these floodwaters could pose a public health risk. The abundance of these virulence factors agrees with previously published qPCR measurements of ARG in samples collected from soils flooded during HH ( Pérez-Valdespino et al, 2021 ), in samples collected within Galveston Bay 2 weeks after HH ( Yang et al, 2021 ), and ARG and pathogens in floodwaters and bayous following HH ( Yu et al, 2018 ).…”

Section: Discussionsupporting

confidence: 88%

Hurricane Harvey Impacts on Water Quality and Microbial Communities in Houston, TX Waterbodies

et al. 2022

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Extreme weather events can temporarily alter the structure of coastal systems and generate floodwaters that are contaminated with fecal indicator bacteria (FIB); however, every coastal system is unique, so identification of trends and commonalities in these episodic events is challenging. To improve our understanding of the resilience of coastal systems to the disturbance of extreme weather events, we monitored water quality, FIB at three stations within Clear Lake, an estuary between Houston and Galveston, and three stations in bayous that feed into the estuary. Water samples were collected immediately before and after Hurricane Harvey (HH) and then throughout the fall of 2017. FIB levels were monitored by culturing E. coli and Enterococci. Microbial community structure was profiled by high throughput sequencing of PCR-amplified 16S rRNA gene fragments. Water quality and FIB data were also compared to historical data for these water body segments. Before HH, salinity within Clear Lake ranged from 9 to 11 practical salinity units (PSU). Immediately after the storm, salinity dropped to < 1 PSU and then gradually increased to historical levels over 2 months. Dissolved inorganic nutrient levels were also relatively low immediately after HH and returned, within a couple of months, to historical levels. FIB levels were elevated immediately after the storm; however, after 1 week, E. coli levels had decreased to what would be acceptable levels for freshwater. Enterococci levels collected several weeks after the storm were within the range of historical levels. Microbial community structure shifted from a system dominated by Cyanobacteria sp. before HH to a system dominated by Proteobacteria and Bacteroidetes immediately after. Several sequences observed only in floodwater showed similarity to sequences previously reported for samples collected following Hurricane Irene. These changes in beta diversity corresponded to salinity and nitrate/nitrite concentrations. Differential abundance analysis of metabolic pathways, predicted from 16S sequences, suggested that pathways associated with virulence and antibiotic resistance were elevated in floodwater. Overall, these results suggest that floodwater generated from these extreme events may have high levels of fecal contamination, antibiotic resistant bacteria and bacteria rarely observed in other systems.

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Section: Discussionsupporting

confidence: 88%

Hurricane Harvey Impacts on Water Quality and Microbial Communities in Houston, TX Waterbodies

et al. 2022

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“…Nevertheless, prediction of ARG and virulence factors with PICRUSt2 did agree with previously published qPCR measurements of ARG in samples collected from soils flooded during HH [41], in samples collected within Galveston Bay two weeks after HH [66], and ARG and pathogens in floodwaters and bayous following HH [67]. Prediction of increase in carbon cycling bacteria similarly agrees with reports that loading of dissolved organic carbon (DOC) during extreme weather events can enhance carbon cycling by bacterial communities in receiving waters [3] and high DOC levels in Galveston Bay following HH [53,65].…”

Section: Discussionsupporting

confidence: 88%

Hurricane Harvey Impacts on Water Quality and Microbial Communities in Houston, TX Waterbodies

LaMontagne

Zhang

Guillen

et al. 2022

Preprint

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Hurricanes, and other extreme weather events, can temporarily alter the structure of coastal systems and generate floodwaters that are contaminated with fecal-associated microbes; however, every coastal system and extreme weather event is unique, so identification of trends and commonalities in these episodic events is challenging. To improve our understanding of the resilience of coastal systems to the disturbance of extreme weather events and the risk exposure to floodwaters poses to the public, we monitored water quality, fecal indicator bacteria (FIB) at three stations within Clear Lake, an estuary between Houston and Galveston, and three stations in bayous that feed into the estuary. Water samples were collected immediately before Hurricane Harvey (pre-HH, August 25th, 2017), immediately after (HH, August 30th) and then throughout the fall of 2017 (post-HH). FIB levels were monitored by culturing E. coli and Enterococci. Microbial community structure was profiled by high throughput sequencing of PCR-amplified 16S rRNA gene fragments. Water quality and FIB data was also compared to historical data for these water body segments. Before Harvey, salinity within Clear Lake ranged from 9 to 11 practical salinity units (PSU). Immediately after the storm, salinity dropped to < 1 PSU and then gradually increased to pre-storm and historical levels over two months. Dissolved inorganic nutrient levels were also relatively low immediately after Harvey and returned, within a couple of months, to pre-HH and historical levels. FIB levels were elevated immediately after the storm; however, after one week, E. coli levels had decreased to acceptable levels for freshwater. Enterococci levels collected several weeks after the storm were within the range of historical levels for these water bodies. Microbial community structure shifted from a system dominated by Cyanobacteria sp. before HH to a system dominated by Proteobacteria and Bacteroidetes immediately after. Further, several sequences observed only in floodwater showed similarity to sequences previously reported for samples collected following Hurricane Irene. These changes in beta diversity corresponded to salinity and nitrate/nitrite concentrations. Differential abundance analysis of metabolic pathways, predicted from 16S sequences, suggested that pathways associated with virulence and antibiotic resistance were elevated in floodwater. Overall, these results suggest that recovery of the Clear Lake system following Hurricane Harvey took at least a month and floodwater generated from these extreme events may have high levels of fecal contamination, antibiotic resistant bacteria and bacteria rarely observed in other systems.

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“…A range of sensitive and specific strategies are developed for the quantification of those compounds in numerous livestock food materials. However, it is pertinent to mention here that the determination of trace residues in foods like milk typically need intensive sample extraction and preparation before conducting instrumental analysis (Pérez-Valdespino et al, 2020). Twenty three (23) out of 90 samples were positive for antibiotic (Penicillin and Oxytetracycline) residues according to techniques applied as mentioned in Table 1 and same technique was used by (Abebew et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Antibiotic Residues of Penicillin and Oxytetracycline in Yoghurt: One Health Needs Paradigm Shift, Legislation and Policy Formulation in Pakistan

Raza,

Durrani,

Bano

et al. 2024

PUJZ

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Yoghurt is considered a nutritional food rich in proteins and other essential minerals. Poor quality milk having Antibiotic Resistance resides threat to one health. Antibiotic Resistance is the main issue that affects the livestock, humans and environment. Antibiotic Resistance occurs due to the use of excessive amount of antibiotics for the growth and development of livestock. 90 samples of each weigh about 50 grams have been collected from Lahore and brought to University of Veterinary and Animal Sciences, Lahore in a Laboratory and applied HPLC technique on samples and found residues of Penicillin and oxytetracycline in different percentages (11% and 9%, respectively) and percentage of both combine Penicillin and oxytetracycline is 3%. In a yoghurt sample, the highest penicillin was found as 13.95 µg/Kg, the lowest penicillin was detected as 7.07 µg/Kg, the highest oxytetracycline was observed as 306.73 µg/Kg, the lowest oxytetracycline was figured out as 197.77 µg/Kg. The result is statistically significant and against the Null Hypothesis. It shows that significant difference has been estimated among all of the positive samples of yogurt. We found the commonly used antibiotics (Penicillin and oxytetracycline) in routine food i.e., in yoghurt samples collected from open market of Lahore, District of Punjab Province, Pakistan.Novelty Statement | The detection of antibiotics (ABs) residues of penicillin and oxytetracycline in yoghurt through HPLC have been detected for the first time in Lahore, Punjab, Pakistan as a research study.

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Impact of flooding on urban soils: Changes in antibiotic resistance and bacterial community after Hurricane Harvey

Cited by 15 publications

References 55 publications

Hurricane Harvey Impacts on Water Quality and Microbial Communities in Houston, TX Waterbodies

Hurricane Harvey Impacts on Water Quality and Microbial Communities in Houston, TX Waterbodies

Hurricane Harvey Impacts on Water Quality and Microbial Communities in Houston, TX Waterbodies

Antibiotic Residues of Penicillin and Oxytetracycline in Yoghurt: One Health Needs Paradigm Shift, Legislation and Policy Formulation in Pakistan

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