Detection of subcellular nitric oxide in mitochondria using a pyrylium probe: assays in cell cultures and peripheral blood

Abstract

A pyrylium based fluorescent probe is able to detect mitochondrial nitric oxide and to discriminate phagocytic from non-phagocytic leukocytes.

Gold nanoparticle-based two-photon fluorescent nanoprobe for monitoring intracellular nitric oxide levels

“…2 Considerable endeavours have been centred on the design of intracellular fluorescent NO molecular probes. 11,[13][14][15][16][17][18][19][20][21] Amongst these, aromatic ortho-diamino (o-diamino)-fluorophore derivatives have attracted special attention and plenty of examples can be found in the literature. [22][23][24][25][26][27][28][29][30] Some of these o-diaminoderivative probes are commercially available and broadly used for the detection of NO in biological samples.…”

Development of a Facile Lysosome-Targeting Nitric Oxide Fluorescent Probe Based on a Novel Reaction Mechanism

“…Among many detection methods reported, fluorescence imaging occupies a very advantageous position in the detection of biological species due to its superiority of convenient use, noninvasive detection, real-time visualization, and low cost. , So far, a number of fluorescent probes have been developed for the detection of NO. , These probes are basically constructed by linking a fluorophore at a certain optical window to a recognition moiety, which specifically reacts with NO under physiological conditions. The reported reactions include o -phenylenediamine (OPD) cyclization, − dihydropyridine (DHP) oxidation, − metal coordination, − etc. − However, the inherent defects of these reactions with NO have greatly limited the biological applications of these probes. For example, fluorescent probes based on OPD and DHP can be oxidized by biologically active species like ascorbic acid (AA), dehydroascorbic acid (DHA), and methylglyoxal (MGO) and produce false positive signals .…”