Detection of stiff nanoparticles within cellular structures by contact resonance atomic force microscopy subsurface nanomechanical imaging

Reggente, Melania; Passeri, Daniele; Angeloni, Livia; Scaramuzzo, Francesca A.; Barteri, Mario; Angelis, Francesca De; Persiconi, Irene; Stefano, Maria Egle De; Rossi, M.

doi:10.1039/c7nr01111c

Cited by 28 publications

(19 citation statements)

References 57 publications

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“…This morphological damage in cancer cells confirms the antitumorigenic activity of the CUR-AuNCs, suggesting their potential for cancer therapy. Passeri et al, used contact resonance AFM (CR-AFM) as a nondestructive subsurface nanomechanical characterization tool for the detection of stiff (magnetic) NPs internalized in cells [ 75 ]. Magnetic NPs were internalized within microglial cells through a phagocytosis process.…”

Section: Toxicity Assessment Of Nanomaterialsmentioning

confidence: 99%

Safety Evaluation of Nanotechnology Products

et al. 2021

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Nanomaterials are now being used in a wide variety of biomedical applications. Medical and health-related issues, however, have raised major concerns, in view of the potential risks of these materials against tissue, cells, and/or organs and these are still poorly understood. These particles are able to interact with the body in countless ways, and they can cause unexpected and hazardous toxicities, especially at cellular levels. Therefore, undertaking in vitro and in vivo experiments is vital to establish their toxicity with natural tissues. In this review, we discuss the underlying mechanisms of nanotoxicity and provide an overview on in vitro characterizations and cytotoxicity assays, as well as in vivo studies that emphasize blood circulation and the in vivo fate of nanomaterials. Our focus is on understanding the role that the physicochemical properties of nanomaterials play in determining their toxicity.

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Section: Toxicity Assessment Of Nanomaterialsmentioning

confidence: 99%

Safety Evaluation of Nanotechnology Products

et al. 2021

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“…;Wang & Zhao, 2012;Liparoti, Sorrentino, Speranza, & Titomanlio, 2017), NanoSwing mode (Sikora, Woszczyna, Friedemann, Ahlers, & Kalbac, 2012;Sikora & Bednarz, 2011). These methods are potentially extremely effective in terms of spatial resolution, range of measurable elastic modulus values, possibility of characterizing both elastic and viscoelastic properties (Killgore et al, 2011b), as well as capability of to detect subsurface features (Cantrell et al, 2007;Killgore, Kelly, Stafford, Fasolka, & Hurley, 2011a;Parlak & Degertekin, 2008;Reggente et al, 2017;Shekhawat & Dravid, 2005). Overall, these methods have been successfully used in a broad range of materials, for example from hard minerals and coatings to soft polymers and biological samples, including food-related materials (Jones, 2016;Rossi et al, 2014).…”

Section: Nanomechanical Characterization Of Food Packaging Surfacesmentioning

confidence: 99%

Atomic Force microscopy techniques to investigate activated food packaging materials

Marinello

Storia

Mauriello

et al. 2019

Trends in Food Science & Technology

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Background: Since its invention, Atomic Force Microscopy has demonstrated to be one of the most interesting and useful techniques in many fields such as biology, optics and electronics to investigate nanoscale phenomena. Not only can it provide high resolution three-dimensional imaging of surfaces, but it also allows quantitative characterization of topographies, forces, mechanical and viscoelastic properties of interfaces at nanometer level. Scope and approach: Here we review current literature in the food packaging field where AFM has been proposed for the quantitative characterization of surfaces with functional layers allowing for exploitation of preservative properties as a result for example of higher permeability or antimicrobial activity. In fact, probing microscopes allow analysis of physical or mechanical properties of interfaces providing relevant information at the nanoscale with regard to different parameters such as dimensions, shapes, evolution and adhesion. Furthermore, recent developments in AFM have shown how fast imaging techniques can be implemented to allow time evolution description even at relatively high temperatures. What we aim is to establish how AFM is effectively promising in the research and development of innovative food packaging. Key findings and conclusions: AFM is largely used to characterize (bio)plastic materials for food packaging but also the comprehension of materials modification due to their activation is approached by using AFM, frequently combined to others instrumental analysis. In particular, even though AFM is basically used for topographical analysis of activated plastic materials, new and advanced AFM analysis are carried out for the characterization of different chemical and physical properties.

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“…The subsurface imaging of magnetite NPs internalized in microglia cells has been obtained by Reggente, Passeri, et al () also using HarmoniX, a technique in which force–distance curves are reconstructed by analyzing the torsional response of a T‐shaped cantilever (Sahin et al, ; Sahin & Erina, ). In this case, however, due to the shallower indentation only stiff NPs close to the surface can be visualized (Passeri, Tamburri, Terranova, & Rossi, ).…”

Section: Identification Of Nanoparticles and Nanosystems In Biological Matricesmentioning

confidence: 99%

Identification of nanoparticles and nanosystems in biological matrices with scanning probe microscopy

Angeloni

Reggente

Passeri

et al. 2018

WIREs Nanomed Nanobiotechnol

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Identification of nanoparticles and nanosystems into cells and biological matrices is a hot research topic in nanobiotechnologies. Because of their capability to map physical properties (mechanical, electric, magnetic, chemical, or optical), several scanning probe microscopy based techniques have been proposed for the subsurface detection of nanomaterials in biological systems. In particular, atomic force microscopy (AFM) can be used to reveal stiff nanoparticles in cells and other soft biomaterials by probing the sample mechanical properties through the acquisition of local indentation curves or through the combination of ultrasound-based methods, like contact resonance AFM (CR-AFM) or scanning near field ultrasound holography. Magnetic force microscopy can detect magnetic nanoparticles and other magnetic (bio)materials in nonmagnetic biological samples, while electric force microscopy, conductive AFM, and Kelvin probe force microscopy can reveal buried nanomaterials on the basis of the differences between their electric properties and those of the surrounding matrices. Finally, scanning near field optical microscopy and tip-enhanced Raman spectroscopy can visualize buried nanostructures on the basis of their optical and chemical properties. Despite at a still early stage, these methods are promising for detection of nanomaterials in biological systems as they could be truly noninvasive, would not require destructive and time-consuming specific sample preparation, could be performed in vitro, on alive samples and in water or physiological environment, and by continuously imaging the same sample could be used to dynamically monitor the diffusion paths and interaction mechanisms of nanomaterials into cells and biological systems. This article is categorized under: Diagnostic Tools > In Vivo Nanodiagnostics and Imaging Nanotechnology Approaches to Biology > Nanoscale Systems in Biology.

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Detection of stiff nanoparticles within cellular structures by contact resonance atomic force microscopy subsurface nanomechanical imaging

Cited by 28 publications

References 57 publications

Safety Evaluation of Nanotechnology Products

Safety Evaluation of Nanotechnology Products

Atomic Force microscopy techniques to investigate activated food packaging materials

Identification of nanoparticles and nanosystems in biological matrices with scanning probe microscopy

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