Detection of specific antibody producing cells in porcine colostrum by in ovo translation of their mRNA

Kortbeek-Jacobs, Netty; Donk, Hans van der

doi:10.1016/0022-1759(78)90101-1

Cited by 9 publications

(1 citation statement)

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“…These IgA* cells are presumed to be committed lymphocytes, that differentiate further into IgAproducing plasma cells once they have reached the mammary gland. A very good tool in the measurement of the productive capacity of cells is in ovo translation of mRNA (Kortbeek-Jacobs and van der Donk, 1978). By applying this method to the same isolated cell populations used for LST~ we obtained data on Ig production after the different immunization schedules (Table V, Fig.…”

Section: Productive Capacitymentioning

confidence: 99%

The effect of oral immunization on the population of lymphocytes migrating to the mammary gland of the sow

Kortbeek-Jacobs

Kooten

Donk

et al. 1984

Veterinary Microbiology

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Kortbeek-Jacobs, J.M.C., van Kooten, P.J.S., van der Donk, J.A., van Dijk, J.E. and Rutten, V.P., 1984. The effect of oral immunization on the population of lymphocytes migrating to the mammary gland of the sow. Vet. Microbiol., 9: 287--299.Sows were immunized orally with live Escherichia coli according to various immunization schedules. Six pregnant gilts were used; 4 immunized at various intervals during the last month of gestation, 1 control immunized after parturition following suppression of lactation by weaning and 1 non-immunized control. The effect of oral vaccination on cell populations from lymphoid organs was studied. The in vitro proliferative responses of the cell populations to K88 antigen, anti-Ig sera and mitogens were used to demonstrate the distribution of sensitized lymphocytes over different lymphoid organs. The capacity of these cells to produce antigen-specific Ig was determined by in ovo translation of their mRNA. Oral administration of antigen resulted in the appearance of K88-positive cells in lymphoid organs. In lactating sows, sensitized cells preferentially occurred in the mammary lymph nodes, whereas after suppression of lactation such a distribution was not seen. A possible route of migration of sensitized lymphocytes is discussed in relation to the local immune response. The antibody isotype produced by sensitized lymphocytes seemed to depend on the immunization schedule. The most effective schedule was one starting early in gestation and comprising frequent administration of antigen. This caused an optimal distribution of sensitized lymphocytes capable of IgA production.

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Section: Productive Capacitymentioning

confidence: 99%