Detection of SNPs in the BMP6 Gene and Their Association with Carcass and Bone Traits in Chicken

Abstract

BMP6, a member of the subfamilies of the morphogenetic proteins (BMPs), plays a crucial role in osteogenic and chondrocyte differentiation in vitro and stimulates chondrogenesis, making chondrocytes differentiate on their terminal stage. The objective of this study is to explore the relationship between polymorphism of BMP6 gene and slaughter traits in chicken respectively. We screened the exonic and intronic regions of BMP6 gene by DNA pool construction and amplified DNA fragment by PCR, and finally, we got nine SNPs. Association analysis revealed that BMP6 had no significant association among all slaughter traits in Yellow bantam chicken. However, BMP6 had a significant difference with femur weight, tibia weight, femur length (p<0.05), and was extremely significant with tibia length (p<0.01) in Avian chicken. Moreover, femur perimeter also had significant correlation with BMP6 in Avian chicken. These results provide useful information for further investigation on the function of chicken BMP6 gene.

The role of BMP6 in the proliferation and differentiation of chicken cartilage cells

“…BMP6 has high levels of expression in the developing growth plate, which is on the contrary with BMP2, BMP4 and BMP7 that are expressed at lower levels or are absent in the growth plate[30,31]. In our previous studies we found that the polymorphism in BMP6 gene in chicken had significant correlation with femur perimeter [32], but the expression of BMP6 in cartilage plate of 15 E, 1 day avian broiler and yellow bantam chickens detected by RT-PCR was high (not published). BMP6 gene plays an important role in the chondrocytes in the chicken embryonic development.…”

Detection of Snps in the Melanocortin 1-Receptor (MC1R) and Its Association with Shank Color Trait in Hs Chicken

“…The concentrations and purity of all DNA samples were assessed by a NanoVuePlus TM spectrophotometer (Thermo Scientific, Wilmington, DE, USA). Based on the concentration of DNA samples determined by the machine, the appropriate amount of Tris-EDTA (TE) buffer was added to achieve a target concentration of 100 ng/mL and all DNA samples were stored at -20 ºC until use (Cui et al, 2017).…”

“…A PCR protocol was used under the following conditions: initial denaturing at 94°C for 5 min, followed by 38 cycles of denaturing at 98°C for 40 s, annealing for 30s at 55°C, and extension at 71°C for 1min. The final extension was performed at 72°C for 5 min (Cui et al, 2017). The PRC products were sequenced by Tsingke Biological Technology (Chengdu, Sichuan).…”