Detection of Simple, Complex, and Clonal Chromosome Translocations Induced by Internal Radioiodine Exposure: A Cytogenetic Follow-Up Case Study after 25 Years

Livingston, Gordon K.; Ryan, Terri L.; Smith, Tammy L.; Escalona, Maria; Foster, Alvis E.; Balajee, Adayabalam S.

doi:10.1159/000504689

Cited by 9 publications

(23 citation statements)

References 42 publications

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“…In the first examined sample (day 1959), among 11.8 CA/100 cells, 61.6% were translocations ( Table 1 ), attributable to the previous exposures of PBL and bone marrow. The observed pattern was close to that described in [ 22 ], a 25-year follow-up study of a DTC patient, which reported 66 translocations in 669 cells, whereas we observed 54 translocations in 526 cells. No clonal aberrations, which reflect early damage to bone marrow stem cells and are defined as at least three cells with identical translocations, were found [ 23 ].…”

Section: Discussionsupporting

confidence: 92%

Cytogenetic Damage Induced by Radioiodine Therapy: A Follow-Up Case Study

Nasonova

Krylov

et al. 2023

IJMS

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The risk of toxicity attributable to radioiodine therapy (RIT) remains a subject of ongoing research, with a whole-body dose of 2 Gy proposed as a safe limit. This article evaluates the RIT-induced cytogenetic damage in two rare differentiated thyroid cancer (DTC) cases, including the first follow-up study of a pediatric DTC patient. Chromosome damage in the patient’s peripheral blood lymphocytes (PBL) was examined using conventional metaphase assay, painting of chromosomes 2, 4, and 12 (FISH), and multiplex fluorescence in situ hybridization (mFISH). Patient 1 (female, 1.6 y.o.) received four RIT courses over 1.1 years. Patient 2 (female, 49 y.o.) received 12 courses over 6.4 years, the last two of which were examined. Blood samples were collected before and 3–4 days after the treatment. Chromosome aberrations (CA) analyzed by conventional and FISH methods were converted to a whole-body dose accounting for the dose rate effect. The mFISH method showed an increase in total aberrant cell frequency following each RIT course, while cells carrying unstable aberrations predominated in the yield. The proportion of cells containing stable CA associated with long-term cytogenetic risk remained mostly unchanged during follow-up for both patients. A one-time administration of RIT was safe, as the threshold of 2 Gy for the whole-body dose was not exceeded. The risk of side effects projected from RIT-attributable cytogenetic damage was low, suggesting a good long-term prognosis. In rare cases, such as the ones reviewed in this study, individual planning based on cytogenetic biodosimetry is strongly recommended.

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Section: Discussionsupporting

confidence: 92%

Cytogenetic Damage Induced by Radioiodine Therapy: A Follow-Up Case Study

Nasonova

Krylov

et al. 2023

IJMS

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“…The baseline frequency of dicentric chromosomes in the general population is estimated to be 0.001/cell (one dicentric chromosome in 1000 metaphase cells [57]). The frequency of dicentric chromosomes detected in the radioiodine patient was 0.02/cell and 0.006/cell, respectively, after 21 and 25 years of the second RIT [53,56]. Although the frequency of dicentric chromosomes declined over the years, it was still sixfold higher than the baseline frequency reported for the normal population.…”

Section: Internal Radioiodine Exposurementioning

confidence: 66%

“…Cancer patients undergoing therapy with radioiodine ( 131 I) provide unique opportunities to investigate the acute and chronic effects of radiation administered under carefully controlled conditions, allowing prospective follow-up studies for extended periods. In our earlier studies, the cytogenetic effects were demonstrated in the peripheral blood lymphocytes of a 34 year old patient [53][54][55][56] who received two rounds of ablative radioactive 131 I therapy (RIT) in 1992 (1.78 GBq; 48 mCi) and 1994 (14.5 GBq; 392 mCi), respectively, after a total thyroidectomy for his papillary thyroid adenocarcinoma. Prior to radioiodine therapy, the patient did not have any occupational or medical exposure to radiation.…”

Section: Internal Radioiodine Exposurementioning

confidence: 99%

Cytogenetic follow-up studies on humans with internal and external exposure to ionizing radiation

Balajee¹,

Livingston²,

Escalona³

et al. 2021

J. Radiol. Prot.

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Cells exposed to ionizing radiation have a wide spectrum of DNA lesions that include DNA single-strand breaks, DNA double-strand breaks (DSBs), oxidative base damage and DNA-protein crosslinks. Among them, DSB is the most critical lesion, which when mis-repaired leads to unstable and stable chromosome aberrations. Currently, chromosome aberration analysis is the preferred method for biological monitoring of radiation-exposed humans. Stable chromosome aberrations, such as inversions and balanced translocations, persist in the peripheral blood lymphocytes of radiation-exposed humans for several years and, therefore, are potentially useful tools to prognosticate the health risks of radiation exposure, particularly in the hematopoietic system. In this review, we summarize the cytogenetic follow-up studies performed by REAC/TS (Radiation Emergency Assistance Center/Training site, Oak Ridge, USA) on humans exposed to internal and external radiation. In the light of our observations as well as the data existing in the literature, this review attempts to highlight the importance of follow-up studies for predicting the extent of genomic instability and its impact on delayed health risks in radiation-exposed victims.

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“…Fluorescence in situ hybridization (FISH)-based translocation assay is a representative methodology to assess stable chromosomal aberrations [32], which could help to study further the impact of their occupational radiation exposure on stable chromosomal damages. Specifically, the 24-multicolor FISH technique would allow evaluating complex chromosome exchanges induced by high LET radiation and thereby find the contribution of neutron exposure to the chromosomal damages of our subjects [33][34][35] Another limitations to this study includes the low number of multiaberrant cells, the inexact criteria for multi-aberrant cells, and the fact that insignificant amounts of neutron exposure contribute to all external effective doses.…”

Section: Discussionmentioning

confidence: 99%

Chromosome Damage in Relation to Recent Radiation Exposure and Radiation Quality in Nuclear Power Plant Workers

Kim

Cho

Choi

et al. 2022

Toxics

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Ionizing radiation is a well-known carcinogen that causes genomic instability. However, the biological and carcinogenetic effects of occupational radiation exposure at low doses have not been extensively studied. The aim of this study was to assess chromosomal instability in power plant workers exposed to occupational radiation at low doses in South Korea. Chromosomal aberrations in the lymphocytes of 201 nuclear power plant workers and 59 sex-matched controls were measured. Chromosomal aberrations in the lymphocytes of 201 nuclear power plant workers (mean age: 41.4 ± 10.0 years) and 59 sex-matched controls (mean age: 47.2 ± 6.0 years) were measured. A total of 500 metaphases for each subject were scored randomly. The means of recent 1.5-year, recent 5.5-year, and cumulative exposed radiation doses among workers were 8.22 ± 7.0 mSv, 30.7 ± 22.0 mSv, and 158.8 ± 86.1 mSv, respectively. The frequency of chromosome-type and chromatid-type aberrations was significantly higher in workers than that in the control group (p < 0.001), and the frequency of chromosome-type aberrations among workers increased in a radiation dose-dependent manner (τ = 0.16, p = 0.005). Poisson regression analyses revealed that chromosome-type aberrations were significantly associated with recent 1.5-year dose after adjusting for confounding variables such as age, smoking, and alcohol intake, even when only the exposed worker was considered. Frequency of multi-aberrant cells (two or more chromosome aberrations within a cell) increased according to cumulative neutron exposure. Our study demonstrates that chromosome damage can be induced in nuclear power plant workers occupationally exposed to ionizing radiation at low doses below the occupational permissible dose limit. Furthermore, an increase in multi-aberrant cells may provide evidence for chronic neutron exposure in nuclear power plant workers. This study was performed to obtain baseline data for a surveillance program of workers occupationally exposed to ionizing radiation long-term.

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Detection of Simple, Complex, and Clonal Chromosome Translocations Induced by Internal Radioiodine Exposure: A Cytogenetic Follow-Up Case Study after 25 Years

Cited by 9 publications

References 42 publications

Cytogenetic Damage Induced by Radioiodine Therapy: A Follow-Up Case Study

Cytogenetic Damage Induced by Radioiodine Therapy: A Follow-Up Case Study

Cytogenetic follow-up studies on humans with internal and external exposure to ionizing radiation

Chromosome Damage in Relation to Recent Radiation Exposure and Radiation Quality in Nuclear Power Plant Workers

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