Detection of siderophore production from several fungi and bacteria by a modification of chrome azurol S (CAS) agar plate assay

Milagres, Adriane M. F.; Machuca, Ángela; Napoleão, Diovana Aparecida dos Santos

doi:10.1016/s0167-7012(99)00028-7

Cited by 282 publications

(168 citation statements)

References 13 publications

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“…Change in color of CAS agar and CAS reagent from blue to orange red indicated the ability of A. feacalis BCCM ID 2374 to produce and excrete siderophores [17].…”

Section: Resultsmentioning

confidence: 99%

Growth and siderophores production in Alcaligenes faecalis is regulated by metal ions

Sayyed¹,

Chincholkar

2010

Indian J Microbiol

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During stationary phase of growth under low stress of iron in succinic acid medium, Alcaligenes feacalis BCCM ID 2374 produced microbial iron chelators. Increase in iron concentration supported bacterial growth but suppressed siderophores production, 1 μM and 2 μM of iron was optimum for maximum siderophore yield, i.e. 354 and 360 μg/ml in untreated and deferrated medium, respectively. Threshold level of iron, which suppressed siderophores production in A. feacalis BCCM ID 2374, was 20 μM. Ten micromoles and above concentration of CuCl 2 and CoCl 2 , and 20 μM of MgCl 2 , MgSO 4 , ZnCl 2 and ZnSO 4 severely affected siderophores production.

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“…Change in color of CAS agar and CAS reagent from blue to orange red indicated the ability of A. feacalis BCCM ID 2374 to produce and excrete siderophores [17].…”

Section: Resultsmentioning

confidence: 99%

Growth and siderophores production in Alcaligenes faecalis is regulated by metal ions

Sayyed¹,

Chincholkar

2010

Indian J Microbiol

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“…The solubilization halo formed after one week of incubation at 30°C was evaluated. Siderophore production was detected according to the protocol described by Milagres et al (1999) using chrome azurol sulphonate (CAS) agar plates. The formation of orange halos on CAS agar plates after incubation for one week at 30°C was evaluated.…”

Section: Vineyards Plant Materials and Experimental Designmentioning

confidence: 99%

Root-associated bacteria promote grapevine growth: from the laboratory to the field

et al. 2016

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Background and Aims Laboratory and greenhouse experiments have shown that root-associated bacteria have beneficial effects on grapevine growth; however, these effects have not been tested in the field. Here, we aimed to demonstrate whether bacteria of different geographical origins derived from different crop plants can colonize grapevine to gain a beneficial outcome for the plant leading to promote growth at the field scale. Methods To link the ecological functions of bacteria to the promotion of plant growth, we sorted fifteen bacterial strains from a larger isolate collection to study in vitro Plant Growth Promoting (PGP) traits. We analysed the ability of these strains to colonise the root tissues of grapevine and Arabidopsis using greenfluorescent-protein-labelled strain derivatives and a cultivation independent approach. We assessed the ability of two subsets randomly chosen from the 15 selected strains to promote grapevine growth in two field-scale experiments in north and central Italy over two years. Parameters of plant vigour were measured during the vegetative season in de novo grafted vine cuttings and adult productive plants inoculated with the bacterial strains. Results Beneficial bacteria rapidly and intimately colonized the rhizoplane and the root system of grapevine. In the field, plants inoculated with bacteria isolated from grapevine roots out-performed untreated plants. In both the tested vineyards, bacteria-promotion effects largely rely in the formation of an extended epigeal system endowed of longer shoots with larger diameters and more nodes than non-inoculated plants.Conclusions PGP bacteria isolated in the laboratory can be successfully used to promote growth of grapevines in the field. The resulting larger canopy potentially increased the photosynthetic surface of the grapevine, promoting growth.

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“…When a strong chelator removes the iron from the dye, its color turns from blue to orange. Milagres et al, (1999) developed a modified method of CAS agar plate assay which was made by incorporating the CAS blue dye in a medium with no contact with the micro-organisms tested. Half of each plate used in our experiments was filled with the most appropriate culture medium for each type of microorganism and the other half with CAS-blue agar.…”

Section: Introductionmentioning

confidence: 99%

Siderophore Producing Aspergillus spp as Bioinoculant for Enhanced Growth of Mung Bean

Patel

Thakar

et al. 2017

IJAAST

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Two fungal species A. niger and A. parasiticus isolated from agriculture field were used in the present study. These two fungal isolates were studied for the siderophore production on the modified CAS agar medium. On daily observation it was observed that the growth was increased along with the production indicating the change in colour of CAS dye from blue to purplish red by A. niger and Yellow by A. parasiticus. Quantitative determination of siderophore production was further studied using liquid medium. After 10 days of incubation A. niger and A. parasiticus produce 80% and 73% units of siderophore. Chemical determination showed that both the fungal isolates showed hydroxamate type of siderophore. Optimization of type of inoculum showed that block inoculum produced maximum siderophore compared to loop and monosporic inoculation in both the fungal isolates. Neutral pH 7.0 showed maximum siderophore production in both the isolates. Decrease in iron concentration increases the siderophore production by the fungal isolates. Maximum siderophore production 80% and 74% units was observed in A. niger and A. parasiticus respectively when there was no addition of iron in the medium. Pot studies were carried out to observe the effect of fungal inoculum on mungbean plant. Inoculation with fungal isolates showed increase in number of leaves, number of branches, number of lateral roots, plant height, fresh weight and dry weight of plant compared to control. Thus both the fungal isolates proved to be potential bioinoculant for improving the growth of mungbean plant.

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Detection of siderophore production from several fungi and bacteria by a modification of chrome azurol S (CAS) agar plate assay

Cited by 282 publications

References 13 publications

Growth and siderophores production in Alcaligenes faecalis is regulated by metal ions

Growth and siderophores production in Alcaligenes faecalis is regulated by metal ions

Root-associated bacteria promote grapevine growth: from the laboratory to the field

Siderophore Producing Aspergillus spp as Bioinoculant for Enhanced Growth of Mung Bean

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