Detection of Shiga-Like (SL) Toxins of Enteropathogenic Escherichia coli (EPEC) of Human, Porcine, Calf, and Lamb Origin on Vero and HeLa S3 Cells: A comparative Study

Baloda, Suraj B.; Yuk, Yu R.; Krovacek, Karel; Sethi, Saurabh; Wadström, Torkel

doi:10.1016/s0176-6724(87)80122-0

Cited by 5 publications

(3 citation statements)

References 23 publications

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“…SLT production has most often been associated with serotypes 0157:H7 and 026:H11 (14,26,30). However, a great amount of serological diversity has been observed among SLT-producing E. coli (1,2,3,11,19,31). Most of the serotypes isolated in this study have not been implicated in enterohemorrhagic colitis or hemolytic uremic syndrome.…”

Section: Discussionmentioning

confidence: 67%

“…was isolated from calves in Sri Lanka (19), and E. coli 0149:H? has been reported from calves with diarrhea in England (1,31). SLT-producing E. coli 02:H7 has also been isolated from animals in Thailand (31).…”

Section: Discussionmentioning

confidence: 99%

“…EHEC is usually found in outbreaks of hemorrhagic colitis and hemolytic uremic syndrome. Although SLT-producing E. coli have been isolated from animals (1,3,11,20,29,31), these E. coli occur infrequently as a cause of endemic diarrhea in both North America and Thailand. In areas with relatively high incidences of childhood diarrhea and dysentery, such as Thailand, outbreaks of EHEC diarrhea may be difficult to recognize.…”

Section: Discussionmentioning

confidence: 99%

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Shiga-like-toxin-producing Escherichia coli in retail meats and cattle in Thailand

Suthienkul

Brown

Seriwatana

et al. 1990

Appl Environ Microbiol

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Specific DNA probes were used to identify Shiga-like toxin I (SLT I)and SLT II-producing Escherichia coli in vegetables, meats, cattle, and farm animals in Thailand. SLT-producing E. coli was isolated from 9% of market beef specimens, from 8 to 28% of fresh beef specimens at slaughterhouses, and from 11 to 84% of fecal specimens from cattle. Animals were frequently infected with several different SLT-producing E. coli types that hybridized with either the SLT I, SLT II, or both SLT probes. Of 119 SLT-producing E. coli isolates, 24% hybridized with the SLT I probe, 31% hybridized with the SLT II probe, and 44% hybridized with both SLT probes. The enterohemorrhagic E. coli plasmid probe hybridized with 64% (68 of 106) of SLT-producing E. coli isolates from food and cattle and with 8% (17 of 201) of E. coli isolates from pigs. No SLT-producing E. coli was detected in pigs. Seventy-six percent (26 of 34) of E. coli isolates that hybridized with the SLT II probe were cytotoxic to Vero but not to HeLa cells, suggesting that they produced the variant of SLT II. The high prevalence of SLT-producing E. coil in beef-producing animals suggests that exposure to animals and eating beef may pose a health risk for acquiring enterohemorrhagic E. coli infections in Thailand.

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Section: Discussionmentioning

confidence: 67%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Shiga-like-toxin-producing Escherichia coli in retail meats and cattle in Thailand

Suthienkul

Brown

Seriwatana

et al. 1990

Appl Environ Microbiol

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Production of cytotoxic necrotizing factor, verocytotoxin and haemolysin by pyelonephritogenicEscherichia coli

Brauner

Katouli

Tullus

et al. 1990

Eur. J. Clin. Microbiol. Infect. Dis.

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Two hundred and thirty-two strains of Escherichia coli isolated from children with non-obstructive acute pyelonephritis (n = 65), women with non-obstructive acute pyelonephritis (n = 63) and the faecal flora of healthy children (n = 33) and adults (n = 71) were examined for cytotoxic necrotizing factor production, haemolysin synthesis, verocytotoxin production and expression of mannose-resistant haemaglutination of human erythrocytes. Forty-eight per cent of the pyelonephritogenic Escherichia coli strains produced cytotoxic necrotizing factor and 61% produced haemolysin compared to 25% and 27% of faecal control strains (p less than 0.001 and p less than 0.001 respectively). Cytotoxic necrotizing factor production did not occur among the non-haemolytic Escherichia coli strains which confirms the close association between these two toxic factors. The bacterial phenotypes producing both haemolysin and cytotoxic necrotizing factor, and the phenotype expressing both these toxic factors and mannose-resistant haemagglutination occurred significantly more often in pyelonephritogenic strains than in faecal isolates (p less than 0.001). Haemolytic strains without the ability to produce cytotoxic necrotizing factor were more common in faecal isolates than in uropathogenic strains (p = 0.05). Strains lacking the ability to synthesize both these toxins were also over-represented in faecal isolates (p less than 0.01).

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Purification and Characterization of a Phage-encoded Cytotoxin from an Escherichia coli O111 Strain Associated with Hemolytic-uremic Syndrome

Karch

Bitzan

1988

Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Seri

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Detection of Shiga-Like (SL) Toxins of Enteropathogenic Escherichia coli (EPEC) of Human, Porcine, Calf, and Lamb Origin on Vero and HeLa S3 Cells: A comparative Study

Cited by 5 publications

References 23 publications

Shiga-like-toxin-producing Escherichia coli in retail meats and cattle in Thailand

Shiga-like-toxin-producing Escherichia coli in retail meats and cattle in Thailand

Production of cytotoxic necrotizing factor, verocytotoxin and haemolysin by pyelonephritogenicEscherichia coli

Purification and Characterization of a Phage-encoded Cytotoxin from an Escherichia coli O111 Strain Associated with Hemolytic-uremic Syndrome

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